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  • Wheat  (6)
  • [abr] RI; Ribonuclease inhibitor  (2)
  • [abr] RNase; Ribonuclease  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of monoclonal antibodies against ribonuclease inhibitor
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 157 (1988), S. 286-294 
    Details
    ISSN: 0006-291X
    Keywords: [abr] BSA; bovine serum albumin ; [abr] IgG; immunoglobulin G ; [abr] RI; Ribonuclease inhibitor ; [abr] RNase; Ribonuclease
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(88)80045-7
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of monoclonal antibodies against ribonuclease inhibitor
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 157 (1988), S. 286-294 
    Details
    ISSN: 0006-291X
    Keywords: [abr] BSA; bovine serum albumin ; [abr] IgG; immunoglobulin G ; [abr] RI; Ribonuclease inhibitor ; [abr] RNase; Ribonuclease
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(88)80045-7
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of additions ofAegilops longissima chromosomes on grain protein in common wheat (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 429-435 
    Details
    ISSN: 1432-2242
    Keywords: Aegilops longissima chromosomes ; Nitrogen fertilization ; Protein content ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of various chromosomes ofAegilops longissima when added to the common wheat cultivar ‘Chinese Spring’ was evaluated at two levels of nitrogen fertilization for absolute and relative amount of protein in the grain. All the added chromosomes ofAe. longissima increased protein percentage: protein increase by chromosomes D, C and A averaged 3.8% while that by chromosomes F, E, G and B averaged 1.7%. Addition lines F, D and C had a significantly higher protein weight per grain. On the other hand, lines A, E and G had reduced grain protein weight per grain as compared with that of ‘Chinese Spring’. Line C carries the HMW glutenin and some of the gliadin subunits ofAe. longissima. The effect of this line, however, and obviously that of the other lines on protein content was through genes controlling the level of storage protein rather than through genes that code directly for these proteins. Nitrogen fertilization affected protein content and the relative amount of the various protein fractions in a similar manner in every addition line. When high levels of nitrogen fertilization were compared to low ones, the relative amount of the HMW glutenins remained constant while that of HMW gliadins increased and that of the LMW subunits decreased. In contrast to the nitrogen effect, increase in protein content by the addition oflongissima chromosomes did not change the relative amounts of the various protein fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00570913
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  • 4
    Electronic Resource
    Electronic Resource
    Evidence for maternal effect in the inheritance of grain protein in crosses between cultivated and wild tetraploid wheats (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 521-524 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Triticum ; Grain protein ; Grain weight ; Maternal effect ; Cytoplasmic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reciprocal crosses were made between cultivated wheat (Triticum turgidum var. ‘durum’) and a high-protein line of wild tetraploid wheat (T. turgidum var. ‘dicoccoides’). F1 grains (on maternal spikes) were very similar to the selfed grains on the maternal parent in protein percentage, weight and protein content. These traits were also analyzed in F3 grains developed on F2 spikes of segregating populations derived from reciprocal crosses between the same cultivated parent and another high-protein line of var. ‘dicoccoides’. No significant differences in the mean values of these traits were found between the reciprocal crosses, indicating no cytoplasmic effect. It has been concluded that these grain characteristics are largely determined by the maternal plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264897
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  • 5
    Electronic Resource
    Electronic Resource
    Identification and chromosomal location of four subfamilies of the rubisco small subunit genes in common wheat (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 385-391 
    Details
    ISSN: 1432-2242
    Keywords: Rubiso small subunit ; Multigene family ; Chromosomal location ; Wheat ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three different 3′ noncoding sequences of wheat rubisco small subunit (SSU) genes (RbcS) were used as probes to identify the gene members of different RbcS subfamilies in the common wheat cultivar Chinese Spring (CS). All genes of the wheat RbcS multigene family were previously assigned to the long arm of homoeologous group 5 and to the short arm of homoeologous group 2 chromosomes of cv CS. Extracted DNA from various aneuploids of these homoeologous groups was digested with four restriction enzymes and hybridized with three different 3′ noncoding sequences of wheat SSU clones. All RbcS genes located on the long arm of homoeologous group 5 chromosomes were found to comprise a single subfamily, while those located on the short arm of group 2 comprised three subfamilies. Each of the ancestral diploid genomes A, B, and D has at least one representative gene in each subfamily, suggesting that the divergence into subfamilies preceded the differentiation into species. This divergence of the RbcS genes, which is presumably accompanied by a similar divergence in the 5′ region, may lead to differential expression of various subfamilies in different tissues and in different developmental stages, in response to different environmental conditions. Moreover, members of one subfamily that belong to different genomes may have diverged also in the coding sequence and, consequently, code for distinguishable SSU. It is assumed that such utilization of the RbcS multigene family increases the adaptability and phenotypic plasticity of common wheat over its diploid progenitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224287
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of a chromosome-specific probe that maps within the Ph1 deletions in common and durum wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 968-970 
    Details
    ISSN: 1432-2242
    Keywords: Key words Chromosome-specific DNA sequence ; Ph1 gene ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Ph1 (pairing homoeologous) gene is the major factor that determines the diploid-like chromosome behavior of polyploid wheat. This gene, which is located on the long arm of chromosome 5B (5BL), suppresses homoeologous pairing at meiosis while allowing exclusive homologous pairing. In an effort to tag the specific chromosomal region where this gene is located, we have previously microdissected chromosome arm 5BL from bread wheat and produced a plasmid library by random PCR amplification and cloning. In this work we isolated from this library a 5BL-specific probe, WPG90, and mapped it within the interstitial deleted chromosome fragments carrying Ph1 in common and durum wheat. A PCR assay of Ph1 based on WPG90 was developed that allows an easy identification of homozygous genotypes deficient for this gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050502
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  • 7
    Electronic Resource
    Electronic Resource
    Chromosomal location of genes for Rubisco small subunit and Rubisco-binding protein in common wheat (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 98-104 
    Details
    ISSN: 1432-2242
    Keywords: Rubisco ; Wheat ; Restriction fragment length polymorphism (RFLP) ; Binding protein ; Gene allocation to chromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes coding for the Rubisco small subunit (SSU) and for the α-subunit of the Rubisco-binding protein were located to chromosome arms of common wheat. HindIII-digested total DNA from the hexaploid cultivar Chinese Spring and from ditelosomic and nullisomic-tetrasomic lines was probed with these two genes, whose chromosomal location was deduced from the disappearance of or from changes in the relative intensity of the relevant band(s). The Rubisco SSU pattern consisted of 14 bands, containing at least 21 different types of DNA fragments, which were allocated to two homoeologous groups: 15 to the short arm of group 2 chromosomes (4 to 2AS, 7 to 2BS, and 4 to 2DS) and 6 to the long arm of group 5 chromosomes (2 on each of arms 5AL, 5BL, and 5DL). The pattern of the Rubisco-binding protein consisted of three bands, each containing one type of fragment. These fragments were located to be on the short arm of group 2 chromosomes. The restriction fragment length polymorphism (RFLP) patterns of several hexaploid and tetraploid lines were highly conserved, whereas the patterns of several of their diploid progenitors were more variable. The variations found in the polyploid species were mainly confined to the B genome. The patterns of the diploids T. monococcum var. urartu and Ae. squarrosa were similar to those of the A and D genome, respectively, in polyploid wheats. The pattern of T. monococcum var. boeoticum was different from the patterns of the A genome, and the patterns of the diploids Ae. speltoides, Ae. longissima, and Ae. Searsii differed from that of the B genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226118
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  • 8
    Electronic Resource
    Electronic Resource
    Yield response of a common spring wheat cultivar to inoculation withAzospirillum brasilense at various levels of nitrogen fertilization (1984)
    Springer
    Plant and soil 80 (1984), S. 255-259 
    Details
    ISSN: 1573-5036
    Keywords: Azospirillum brasilense ; Wheat ; Triticum aestivum ; N-fertilizer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The yield response of a common spring wheat cultivar,Triticum aestivum, to inoculation withAzospirillum brasilense was studied at four levels of N fertilization. Plant yield increased due to the inoculation treatment only at medium and high levels of N fertilization, with a maximum yield increase of about 8.0 per cent at the highest level (approximately 1.0 g of pure N per plant). Yield increase was mostly due to an increase in the number of grains per spike, and at the highest level of fertilization, also due to a higher number of spikes per plant. At all N levels, the inoculation caused an increase of 0.5–1.4 per cent in the number of fertile spikelets per main spike. Grain protein percentage was unaffected by the inoculation, though significantly increase due to the fertilization treatments. The occurrence of maximum yield response at the highest N level, the response by early-determined yield components, i.e. spikelet number, and the unaffected grain protein content are in accord with the suggestion that the contribution ofAzospirillum brasilense to wheat yield is not through N2-fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02161181
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