ISSN:
1432-1890
Keywords:
Arbuscule-like structures (ALS)
;
Branched absorbing structures (BAS)
;
Dual culture
;
Glomus caledonium
;
Sporulation
;
Transformed carrot roots
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Glomus caledonium was established in a dual culture with Ri T-DNA-transformed carrot roots. A modification of the minimal M medium buffered at pH 6.50 with 10 mM MES and solidified with 0.4% unpurified gellan gum allowed spore germination and formation of the symbiosis, together with the development of an extensive extramatrical mycelium and sporulation. Spore production increased with culture generation and most spores were viable. These spores colonized carrot roots and completed the fungal life cycle. In many cultures, sporulation was accompanied by the formation of arbuscule-like structures on short and thickened lateral branches of main hyphae. Root colonization was of the Paris-type with hyphae spreading intracellularly. Most colonized root cells contained coils of thickened hyphae, sometimes surrounded by fine hyphae, but no typical arbuscules were observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s005720050283
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