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  • Rutilus frisii kutum  (8)
  • 1
    Publication Date: 2021-05-19
    Description: In this study lusiferse gene extracted from Vibrio fischeri. V. fischeri strain was kindly provided by Iranian Research Organization for Science and Technology (IROST). Genomic DNA extraction was carried out by Phenol-chloroform. A DNA fragment encoding the luxA and LuxB was amplified by PCR using sense and antisense primers, specific restriction sites for BamH1 and Kpn1 were introduced into 5´ end of forward and reverse primer, respectively. The PCR product was purified from agarose gel and ligated into cleaved PT257R cloning vector. Following the confirmation of the cloned Luciferase transformed into E. coli. Recombinant clones were confirmed by specific PCR and restriction enzyme digestion analysis. The luxA and LuxB fragment was released subcloned in to the PcDNA3.1\hug and PcDNA3.1\neo expression vectors, respectively. recombinant plasmid was confirmed through restriction digestion using BamH1 and Kpn1 enzymes and subsequently, transformation procedure continued into NIH3T3 eukaryote cells by specific kit. Luminescence ability of recombinant clones was tested by NIH3T3 cells and dechanal (substrate) and Neomysin and hygromysin. The results showed that luminescence start after 2 hours and then increase after 6 hours. Inaddition, the protein identity was verified by western blot analysis, the protein bands 76 kD were detected. , which indicates protein expression of luxB, luxA.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Lusifrase ; Vibrio fischeri ; Transfer gene ; DNA ; Lusiferase gene ; Investihation ; Genetic ; Cyprins carpio ; Rutilus frisii kutum
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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  • 2
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25639 | 18721 | 2018-10-08 05:54:55 | 25639 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study lusiferse gene extracted from Vibrio fischeri. V. fischeri strain was kindly provided by Iranian Research Organization for Science and Technology (IROST). Genomic DNA extraction was carried out by Phenol-chloroform. A DNA fragment encoding the luxA and LuxB was amplified by PCR using sense and antisense primers, specific restriction sites for BamH1 and Kpn1 were introduced into 5´ end of forward and reverse primer, respectively. The PCR product was purified from agarose gel and ligated into cleaved PT257R cloning vector. Following the confirmation of the cloned Luciferase transformed into E. coli. Recombinant clones were confirmed by specific PCR and restriction enzyme digestion analysis. The luxA and LuxB fragment was released subcloned in to the PcDNA3.1\hug and PcDNA3.1\neo expression vectors, respectively. recombinant plasmid was confirmed through restriction digestion using BamH1 and Kpn1 enzymes and subsequently, transformation procedure continued into NIH3T3 eukaryote cells by specific kit. Luminescence ability of recombinant clones was tested by NIH3T3 cells and dechanal (substrate) and Neomysin and hygromysin. The results showed that luminescence start after 2 hours and then increase after 6 hours. Inaddition, the protein identity was verified by western blot analysis, the protein bands 76 kD were detected which indicates protein expression of luxB, luxA.
    Keywords: Biology ; Iran ; Lusifrase ; Vibrio fischeri ; Transfer gene ; DNA ; Lusiferase gene ; Investihation ; Genetic ; Cyprins carpio ; Rutilus frisii kutum
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 58
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  • 3
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25640 | 18721 | 2018-10-14 02:39:07 | 25640 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study, 11 male of Caspian trout (Salmo truta caspius) (with mean length and weight 37/8 ± 5/3 cm and 523/3 ± 24/7 respectively) and 23 male of Caspian kutum (Rutilus frisii kutum) (with mean length and weight 36/1 ± 7/1 cm and 631/3 ± 21/6 g respectively) were evaluated. All the fish were good at the initial examination of sexual maturity. After sperm sampling, their quality were tested. In this step, the parameters such as motility, duration of mobility, density, pH and osmolality were measured. After this stage, the sperm samples of Caspian trout in the ratio 1: 3 were diluted with the aqueous solution containing compounds (0.3M Glucose, 10% Methanol, 10% egg yolk) and the freezing process was done manually and the sperm was frozen in liquid nitrogen. The sperm samples of Caspian kutums were diluted (ratio of 1: 3) with two soluble diluent containing compounds (350 mM glucose, 30 mM Tris and 4% Polyethylene glycol) and (350 mM glucose, 30 mM Tris and 2% Glycerol) and were frizzed automatically by Planner Kryo instrument and placed in liquid nitrogen. The sperm samples were thawed 1 to 3 months after the date of first freezing and their quality were assessed by measuring percent and timing motility. The results showed that the obtained semen volume of Caspian trout was more than Caspian kutum. Moreover, percentage of motile sperm, timing motility and sperm density of Caspian trout were higher than those of Caspian kutum but osmolality and pH of Caspian trout were lower than those of Caspian kutum. Over time, the percentage of sperm motility and mobility for both species declined compared with fresh samples. After thawing, percentage of motile sperm and timing motility of Caspian kutum were lower than those factors Caspian trout. The results showed that the sample of Caspian kutum sperm that were diluted by ethylene glycol after thawing and were immotile ll of them. However, the samples were diluted by glycerol, after thawing, were alive and motile. According to the results, it seems very important species differences that must be fully considered in the process of freezing sperm. The use of a single protocol would not be successful in cryopreservation because the reaction of sperm against to chemical agents is variable. Therefore, it is essential to get the right information to protect valuable Caspian fish by using cryopreservation. Further studies on the characteristics of each species, as well as the freezing process take appropriate diluent.
    Keywords: Aquaculture ; Biology ; Iran ; Salmo truta caspius ; Caspian trout ; Rutilus frisii kutum ; Caspian kutum ; Sperm ; Bony fish ; Cryopreservation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 38
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  • 4
    facet.materialart.
    Unknown
    In:  http://aquaticcommons.org/id/eprint/21895 | 18721 | 2018-01-15 14:06:21 | 21895 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-04
    Description: The main objective of the present study was qualitative assessment of Caspian kutum (Rutilus frisii kutum Kamensky 1901) stocks by using indicators of overfishing, condition factor and relative weight in Iranian waters of the Caspian Sea during a long period (1991- 2011). Three fisheries indicators in catch were: 1) percentage of mature fish, with 100% as target; 2) percent of specimens with optimum length, with 100% as target; and 3) percentage of mega-spawners, with 0% as target, and if no upper size limit exists, 30-40% as representative of reasonable stock structure. Over this period, the condition factor and relative weight had a decreasing trend. The range of length with optimum yield was between 40 and 48 cm. The mega-spawner measured as fish a size larger than 48 cm. According to these three indicators, the percentage of mature (fork length〉40cm), optimum size and mega-spawners were 62.0, 40.9 and 12.5% in 1991-92 which decreased less than 50, 38.2 and 8%, in the years 1992-2011, respectively. The length structure is a matter of concern. Therefore, for reservation and rebuilt of kutum in the Caspian Sea, the target was to let all (100%) fish spawn at least once, using bigger mesh size in cod end (e.g. 35mm, during whole fishing season). The aim was also to implement a fishing strategy that result no (0%) mega-spawners being caught, and the main catch focus on optimum length.
    Keywords: Biology ; Fisheries ; Stock assessment ; Fisheries indicators ; Spawner fish ; Caspian Sea ; Iran ; Rutilus frisii kutum
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 53-64
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  • 5
    Publication Date: 2021-07-16
    Description: Present study analyzed the stomach contents of individuals belonging to five fish’s species in order toInvestigate seasonal changes in feeding strategy and diet composition in the South coast of the Caspian Sea (Gilan and Mazandaran provinces) from late March until late May 2010 using the research ship Gillan and fishing with batoom trawl nets and fishing blades insoles bony fishes in the depths of less than 60 meters. The average length and the total weight of Rutilus frisii kutum, Rutilus rutilus caspicus, Cyprinus carpio, Liza auratus and Liza saliens respectively were 36/9 ± 8/5 cm , 562/9 ± 353/2 g, 23/2 ± 3/2 cm , 202 ± 101/6 g, 24/6 ± 8/1 cm , 249/8 ± 242 g, 32/2 ± 8/9 cm , 320/9 ± 242/8 g, 22/7 ± 2/6 cm and 82/9 ± 52/2 g repectively. Using the method of Shorygin and Costello highly nourishing food strategy and different species of fishes were studied. The results of this study showed that Rutilus frisii kutum fed generally of Balanus and Cerastorderma In order of importance after Bivalvia, Gastropoda, Crabs, Fish eggs, Filamentous alge in GI Rutilus frisii kutum presence, but feeding it of Bivalvia (in particular Cerastorderma) was much more than the other food. The results of present study showed that under the influence of compatibility and behavioral mechanisms, ecological, physiological and nutritional importance of Rutilus frisii kutum Bivalvia. The results of studies were showed that Bivalvia was feeding the dominant Rutilus frisii kutum , while the results of this study showed that despite Bivalvia present importance in feeding the of this species, it seems that its presence in such digestion decreased. As well as the dominant food of Rutilus rutilus caspicus was fish, but generally from Detrite fed. This study showed that feeding the dominant species of Cyprinus carpio, Liza auratus and Liza saliens of Detrite was. The most important food items after ditrit for the Cyprinus carpio in order of importance include the Bivalvia, Gastropoda, Oligochaeta, Abra ovata, Nereis, Fish eggs, Filamentous alge, was. The Liza auratus dietary habits also showed that after ditrit the most importance in nutrition related to Foraminifera, Porifera and Balanus, and for Liza saliens to Gastropoda, Bivalvia and Porifera. The results showed that the mullet the greater the intensity of feeding fishes than other species were, Rutilus frisii kutum in between of Cyprinidae the greater the intensity of feeding fishes than other species were (0/05 〉 P). The values of this indicator in the lower longitudinal in all fish species investigated were more than high longitudinal groups. The feeding intensity at different times of sampling for all species investigated showed that in all species at times extremely reduced the reproductive index (0/05 〉 P). As well as the diversity foods in the lower longitudinal in all fish species investigated were more than high longitudinal groups. In conclusion, our results demonstrated Food strategy have changed based on physiological changes, metabolism activity, behaviour, and the amount of food available.
    Keywords: Aquaculture ; Iran ; Caspian Sea ; Dietary habits ; Rutilus frisii kutum ; Cyprinus carpio ; Rutilus rutilus caspicus ; Mullets ; Survey ; Feeding ; Benthic ; Liza auratus ; Liza saliens
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 46
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  • 6
    Publication Date: 2021-05-19
    Description: Present study analyzed the stomach contents of individuals belonging to five fish’s species in order toInvestigate seasonal changes in feeding strategy and diet composition in the South coast of the Caspian Sea (Gilan and Mazandaran provinces) from late March until late May 2010 using the research ship Gillan and fishing with batoom trawl nets and fishing blades insoles bony fishes in the depths of less than 60 meters. The average length and the total weight of Rutilus frisii kutum, Rutilus rutilus caspicus, Cyprinus carpio, Liza auratus and Liza saliens respectively were 36/9 ± 8/5 cm , 562/9 ± 353/2 g, 23/2 ± 3/2 cm , 202 ± 101/6 g, 24/6 ± 8/1 cm , 249/8 ± 242 g, 32/2 ± 8/9 cm , 320/9 ± 242/8 g, 22/7 ± 2/6 cm and 82/9 ± 52/2 g repectively. Using the method of Shorygin and Costello highly nourishing food strategy and different species of fishes were studied. The results of this study showed that Rutilus frisii kutum fed generally of Balanus and Cerastorderma In order of importance after Bivalvia, Gastropoda, Crabs, Fish eggs, Filamentous alge in GI Rutilus frisii kutum presence, but feeding it of Bivalvia (in particular Cerastorderma) was much more than the other food. The results of present study showed that under the influence of compatibility and behavioral mechanisms, ecological, physiological and nutritional importance of Rutilus frisii kutum Bivalvia. The results of studies were showed that Bivalvia was feeding the dominant Rutilus frisii kutum , while the results of this study showed that despite Bivalvia present importance in feeding the of this species, it seems that its presence in such digestion decreased. As well as the dominant food of Rutilus rutilus caspicus was fish, but generally from Detrite fed. This study showed that feeding the dominant species of Cyprinus carpio, Liza auratus and Liza saliens of Detrite was. The most important food items after ditrit for the Cyprinus carpio in order of importance include the Bivalvia, Gastropoda, Oligochaeta, Abra ovata, Nereis, Fish eggs, Filamentous alge, was. The Liza auratus dietary habits also showed that after ditrit the most importance in nutrition related to Foraminifera, Porifera and Balanus, and for Liza saliens to Gastropoda, Bivalvia and Porifera. The results showed that the mullet the greater the intensity of feeding fishes than other species were, Rutilus frisii kutum in between of Cyprinidae the greater the intensity of feeding fishes than other species were (0/05 〉 P). The values of this indicator in the lower longitudinal in all fish species investigated were more than high longitudinal groups. The feeding intensity at different times of sampling for all species investigated showed that in all species at times extremely reduced the reproductive index (0/05 〉 P). As well as the diversity foods in the lower longitudinal in all fish species investigated were more than high longitudinal groups. In conclution, our results demonstrated Food strategy have changed based on physiological changes, metabolismi activity, behaviour, and the amount of food available.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Dietary habits ; Rutilus frisii kutum ; Cyprinus carpio ; Rutilus rutilus caspicus ; Mullets ; Survey ; Feeding ; Benthic ; Liza auratus ; Liza saliens
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 46pp.
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  • 7
    Publication Date: 2021-05-19
    Description: The main objective of the present study was qualitative assessment of Caspian kutum (Rutilus frisii kutum Kamensky 1901) stocks by using indicators of overfishing, condition factor and relative weight in Iranian waters of the Caspian Sea during a long period (1991- 2011). Three fisheries indicators in catch were: 1) percentage of mature fish, with 100% as target; 2) percent of specimens with optimum length, with 100% as target; and 3) percentage of mega-spawners, with 0% as target, and if no upper size limit exists, 30-40% as representative of reasonable stock structure. Over this period, the condition factor and relative weight had a decreasing trend. The range of length with optimum yield was between 40 and 48 cm. The mega-spawner measured as fish a size larger than 48 cm. According to these three indicators, the percentage of mature (fork length〉40cm), optimum size and mega-spawners were 62.0, 40.9 and 12.5% in 1991-92 which decreased less than 50, 38.2 and 8%, in the years 1992-2011, respectively. The length structure is a matter of concern. Therefore, for reservation and rebuilt of kutum in the Caspian Sea, the target was to let all (100%) fish spawn at least once, using bigger mesh size in cod end (e.g. 35mm, during whole fishing season). The aim was also to implement a fishing strategy that result no (0%) mega-spawners being caught, and the main catch focus on optimum length.
    Description: Published
    Keywords: Rutilus frisii kutum ; Caspian kutum ; Stock assessment ; Fisheries indicators ; Spawner fish ; Fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.53-64
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  • 8
    Publication Date: 2021-12-05
    Description: In this study, 11 male of Caspian trout (Salmo truta caspius) (with mean length and weight 37/8 ± 5/3 cm and 523/3 ± 24/7 respectively) and 23 male of Caspian kutum (Rutilus frisii kutum) (with mean length and weight 36/1 ± 7/1 cm and 631/3 ± 21/6 g respectively) were evaluated. All the fish were good at the initial examination of sexual maturity. After sperm sampling, their quality were tested. In this step, the parameters such as motility, duration of mobility, density, pH and osmolality were measured. After this stage, the sperm samples of Caspian trout in the ratio 1: 3 were diluted with the aqueous solution containing compounds (0.3M Glucose, 10% Methanol, 10% egg yolk) and the freezing process was done manually and the sperm was frozen in liquid nitrogen. The sperm samples of Caspian kutums were diluted (ratio of 1: 3) with two soluble diluent containing compounds (350 mM glucose, 30 mM Tris and 4% Polyethylene glycol) and (350 mM glucose, 30 mM Tris and 2% Glycerol) and were frizzed automatically by Planner Kryo instrument and placed in liquid nitrogen. The sperm samples were thawed 1 to 3 months after the date of first freezing and their quality were assessed by measuring percent and timing motility. The results showed that the obtained semen volume of Caspian trout was more than Caspian kutum. Moreover, percentage of motile sperm, timing motility and sperm density of Caspian trout were higher than those of Caspian kutum but osmolality and pH of Caspian trout were lower than those of Caspian kutum. Over time, the percentage of sperm motility and mobility for both species declined compared with fresh samples. After thawing, percentage of motile sperm and timing motility of Caspian kutum were lower than those factors Caspian trout. The results showed that the sample of Caspian kutum sperm that were diluted by ethylene glycol after thawing and were immotile ll of them. However, the samples were diluted by glycerol, after thawing, were alive and motile. According to the results, it seems very important species differences that must be fully considered in the process of freezing sperm. The use of a single protocol would not be successful in cryopreservation because the reaction of sperm against to chemical agents is variable. Therefore, it is essential to get the right information to protect valuable Caspian fish by using cryopreservation. Further studies on the characteristics of each species, as well as the freezing process take appropriate diluent.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Salmo truta caspius ; Caspian trout ; Rutilus frisii kutum ; Caspian kutum ; Sperm ; Bony fish ; Cryopreservation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 38pp.
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