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  • Rhizobium leguminosarum  (1)
  • birdsfoot trefoil  (1)
  • 1
    ISSN: 1432-0789
    Keywords: Key words Root nodule ; Endophyte ; Rhizobium ; Allelopathy ; Biodiversity ; Red clover ; Agrobacterium rhizogenes ; Rhizobium leguminosarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of this study was to identifiy the endophytic bacteria recovered from the foliage, tap roots and nodules of red clover plants (Trifolium pratense L.); and to assess the effects of the nodule bacteria, alone and in combination with Rhizobium spp., on the growth and development of red clover seedlings. Thirty-one bacteria species from 14 different genera were recovered from within the foliage, roots and nodules of red clover plants cv. AC Charlie. Genera diversity and species number were greatest in foliage tissues. Pantoea agglomerans (59.6%) was the most frequent species recovered in foliage tissues, Agrobacterium rhizogenes A in the tap root (49.2%) and Rhizobium leguminosarum BV phaseoli and R. loti B in the nodules (27.2% each). Recovery of Rhizobium species was not restricted to the nodules, and species of this genus were systemic throughout the plant. Clover root nodules were host to 12 bacteria species other than rhizobia, of which 8 were specific to this tissue. Using non-selective media, R. leguminosarum BV trifolii constituted only 8.8% of all the root nodule bacteria recovered. In root bacterization experiments, species of nodule bacteria promoted growth of red clover more often when applied in combination with R. leguminosarum BV trifolii than when applied singly. However, Bacillus megaterium, Bordetella avium and Curtobacterium luteum consistently promoted growth either individually or in combination with R. leguminosarum BV trifolii. Nodulation was promoted when R. leguminosarum BV trifolii was coinoculated with Bacillus insolitus, B. brevis or A. rhizogenes A. Single isolate applications of Rhizobium species to roots always led to the depression of clover growth, but mixtures of R. leguminosarum BV trifolii and R. leguminosarum BV phaseoli resulted in growth promotion. The latter is considered further evidence of the beneficial allelopathic side effect of strain competition for the same ecological niche.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Lotus corniculatus ; birdsfoot trefoil ; quantitative variability ; days to flowering ; forage grading ; plant height ; seed yield ; seed size ; seeds per pod ; pods per inflorescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Quantitative variability for seed yield and six other characters was analysed in Lotus corniculatus L. cv. Leo. The material consisted of 144 polycross progenies and 100 parents. Wide variability existed for all characters. The characters with the greatest variability were seed yield, forage grading and plant height. The polycross progeny test was employed to study the general combining ability of the parents. Highly significant differences existed for all seven characters under study. Parent-offspring genotypic and phenotypic correlations were high and significant for all characters except genotypic correlations for seed yield and seeds per pod. High h2 values (broad sense) were obtained for seed size and days to flowering. Traits with moderate to high h2 were seed yield (71% in parents, 64% in progenies), plant height, forage grading, and seeds per pod. The character pods per inflorescence had the lowest h2. Positive estimates of % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGak0dh9WrFfpC0xh9vqqj-hEeeu0xXdbba9frFj0-OqFf% ea0dXdd9vqaq-JfrVkFHe9pgea0dXdar-Jb9hs0dXdbPYxe9vr0-vr% 0-vqpWqaaeaabiGaaiaacaqabeaadaqaaqaaaOqaaiqabo8agaqcaK% aaavaabeqaceaaaeaacaqGYaaabaGaaeiraaaaaaa!3A89!\[{\text{\hat \sigma }}\begin{array}{*{20}c} {\text{2}} \\ {\text{D}} \\ \end{array} \] were obtained only for seed size. The ratio of dominance variance to additive variance indicated partial dominance for this character. Except for seed yield, in all other cases these estimates had very high sampling errors. In all cases except pods per inflorescence and seeds per pod high positive estimates of % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGak0dh9WrFfpC0xh9vqqj-hEeeu0xXdbba9frFj0-OqFf% ea0dXdd9vqaq-JfrVkFHe9pgea0dXdar-Jb9hs0dXdbPYxe9vr0-vr% 0-vqpWqaaeaabiGaaiaacaqabeaadaqaaqaaaOqaaiqbeo8aZzaaja% qbaeqabiqaaaqcaauaaiaaikdaaKaaGeaacaqGbbaaaaaa!3B30!\[\hat \sigma \begin{array}{*{20}c} 2 \\ {\text{A}} \\ \end{array} \] were obtained. The data indicated that it may be possible to simultaneously improve seed yield and maintain forage yield. Seed yield had positive and significant associations with seed size, seeds per pod and pods per inflorescence. The associations of days to flowering with forage grading (negative) and with pods per inflorescence (positive) were also significant.
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