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  • 1
    ISSN: 1573-5028
    Keywords: auxin ; cell suspension ; cytokinin ; ribosomal protein ; tobacco ; wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNA clones, TSC29 and TSC40, were isolated from a cDNA library prepared from three-day-old tobacco cell suspension grown to early exponential stage. DNA sequence analyses and database searches revealed that the TSC29 transcript encodes a protein which is highly homologous to eukaryotic 60S ribosomal (r)-protein L25 and that the TSC40 product is homologous to rat 60S r-protein L34. Southern blot analysis showed that the putative r-protein genes are members of multigene families. Transcript levels of both genes were most abundant in three-day-old cell suspension and declined in older cultures. Transcripts were also present in plant vegetative and reproductive organs. However, for TSC40 in particular, the mRNA levels were lower in plant organs than in three-day-old cell suspension. Stems and roots exhibited higher expression than leaves and flowers, indicating that these clones are differentially regulated in various cell types. Both genes were expressed at low levels in mature seeds but transcript levels significantly increased after one day of germination, remained at a high level until day 4, and declined after day 5.in situ localization experiments with germinating seedlings revealed that the TSC29 transcript was preferentially localized in root tips, epidermis, and endosperm. Wounding increased the steady-state mRNA amounts of these r-protein genes, and 2,4-dichlorophenoxyacetic acid and benzyladenine further increased the transcript level.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: genetic diversity ; Triticum aestivum ; Triticum aestivum ssp. tibetanum ; Triticum spelta ; RAPD marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An endemic hexaploid wheat found in Tibet, China was taxonomically classified as a subspecies in common wheat, i.e. Triticum aestivum ssp. tibetanum. Seven accessions of the Tibetan wheat, 22 cultivars of common wheat and 17 lines of spelt wheat were used for RAPD analysis to study the genetic relationships of the Tibetan wheat with common wheat and spelt wheat, and to assess the genetic diversity (GD) among and within the taxa. RAPD polymorphism was found to be much higher within spelt wheat and the Tibetan wheat than within common wheat. The GD value between the Tibetan wheat and common wheat is lower than that between the Tibetan wheat and spelt wheat. The result of cluster analysis showed that the 46 genotypes were distinctly classified into two groups. Group 1 included all European spelt wheat lines, while group 2 includes all Chinese common wheat and the Tibetan wheat accessions. However, the Tibetan wheat was substantially differentiated from Chinese common wheat at a lower hierarchy. Our results support an earlier classification of the Tibetan wheat as a subspecies in common wheat. European spelt wheat and the Tibetan wheat showed much higher genetic diversity than Chinese common wheat, which could be used to diversify the genetic basis for common wheat breeding.
    Type of Medium: Electronic Resource
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