ISSN:
1432-072X
Keywords:
Rhodopseudomonas sphaeroides f. denitrificans
;
Photodenitrifier
;
Denitrification
;
Nitrate reductase
;
Nitrite reductase
;
Nitrous oxide reductase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Rhodopseudomonas sphaeroides f. denitrificans grown photosynthetically with NO 3 - under anaerobic conditions accumulated NO 2 - in the culture medium. In washed cells succinate, lactate, fumarate, citrate and malate, were effective electron donors for the reduction of NO 3 - , NO 2 - and N2O to N2 gas. Nitrate reductase was inhibited by amytal and potassium thocyanate. Nitrite reductase activity was severely restricted by potassium cyanide, sodium diethyldithiocarbamate, Amytal and 2-n-heptyl-4-hydroxyquinoline-N-oxide whereas N2O reductase was inhibited by NaN3, C2H2 and KCNS. Cells incubated with either K15NO3 or K15NO2 produced 15N2O and 15N2. A stoichiometry of 2:1 was recorded for the reduction of either NO 3 - or NO 2 - to N2O and N2 and for N2O to N2 it was 1:1.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446740
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