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A reappraisal of antigenic determinants for nitrogenase from Azotobacter and nitrate reductase from Escherichia coli

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Abstract

Previous work based on double immunodiffusion assays had shown that there are common antigenic determinants for nitrate reductase from Escherichia coli and component I of nitrogenase from Azotobacter vinelandii. Further work reported herein using a variety of immunoelectrophoretic techniques indicates that the cross-reaction between nitrate reductase and antiserum to component I of nitrogenase results from a contaminant antigen co-purified with nitrate reductase.

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Byrne, M.D., Nicholas, D.J.D. A reappraisal of antigenic determinants for nitrogenase from Azotobacter and nitrate reductase from Escherichia coli . Arch. Microbiol. 132, 304–307 (1982). https://doi.org/10.1007/BF00413379

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  • DOI: https://doi.org/10.1007/BF00413379

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