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  • Solanum tuberosum  (6)
  • potato  (3)
  • Lymnaea stagnalis  (2)
  • size grade  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning of G protein α subunits from the central nervous system of the mollusc Lymnaea stagnalis
    Amsterdam : Elsevier
    FEBS Letters 314 (1992), S. 215-219 
    Details
    ISSN: 0014-5793
    Keywords: Central nervous system ; G protein α subunit ; Lymnaea stagnalis ; cDNA cloning
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(92)81474-Z
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  • 2
    Electronic Resource
    Electronic Resource
    Localization of ovulation hormone-like neuropeptide in the central nervous system of the snail Lymnaea stagnalis by means of immunocytochemistry and in situ hybridization (1988)
    Springer
    Cell & tissue research 251 (1988), S. 477-484 
    Details
    ISSN: 1432-0878
    Keywords: Caudo-dorsal cells ; Neuropeptides ; Immunocytochemistry ; In situ hybridization ; Ovulation hormone ; Lymnaea stagnalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The caudo-dorsal cells (CDC) in the cerebral ganglia of the pond snail Lymnaea stagnalis synthesize the 36-amino acid ovulation hormone (CDCH). We have used immuno-cytochemistry and in situ hybridization to reveal the localization of neurons and axons containing CDCH-like material. A monoclonal antibody to a fragment of CDCH and a cDNA probe encoding CDCH reacted with the CDC-system, with specific cell groups in the cerebral and pleural ganglia, and with individually occurring neurons throughout the central nervous system. The cells in the pleural ganglia, which were found in about 50% of the preparations studied, are considered as “ectopic” CDC. They are morphologically similar to CDC in their somal dimensions and axonal organization. By means of immuno-electron microscopy it was shown that these neurons contain secretory vesicles that are similar to those of the CDC. The neurons of the bilateral groups occurring in the cerebral ganglia in addition to the CDC are smaller and more intensely stained than the CDC. Axons of these small neurons probably have varicosities located on the CDC axons in the neuropil of the cerebral ganglion, indicating synaptic contacts. Two major axon tracts could be followed from (or toward) the neuropil of the cerebral ganglion. One tract runs from the cerebral gangion via the pleural and parietal ganglia to the visceral ganglion, giving off branches to most nerves emanating from these ganglia. The other tract could be traced through the cerebro-pedal connective to the pedal ganglia. Only in the right pedal ganglion was extensive axonal branching observed. The nerves emanating from this ganglion contained many more immunoreactive axons than those from the left pedal ganglion. A polyclonal antibody raised against the synthetic fragment of CDCH stained, in addition to the neurons and axons revealed with the monoclonal antibody and the cDNA probe, three other major groups of neurons. Two are located in the cerebral ganglion, the other in the left pedal ganglion. The present findings suggest the presence of a system of neurons that contain CDCH or CDCH-like peptides. The role this system may play in the control of egg-laying and egg-laying behaviour is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215857
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  • 3
    Electronic Resource
    Electronic Resource
    Gibberellins and tuberization in potato (1999)
    Springer
    Potato research 42 (1999), S. 471-481 
    Details
    ISSN: 1871-4528
    Keywords: Solanum tuberosum ; S. andigena ; S. demissum ; hormones ; regulation ; starch accumulation ; tuber formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The evidence for a role of gibberellins in the regulation of potato tuber formation is reviewed. Endogenous gibberellin levels in plants are high under non-inducing conditions and decrease under inducing conditions. Exogenously applied gibberellins inhibit tuber formation, whereas applying inhibitors of gibberellin biosynthesis has the opposite effect. Cellular events involved in tuberization, viz., cell division, cell enlargement and orientation of micro-tubules, are also reviewed. Based on available evidence, a major regulatory role of gibberellins is suggested. However, it is also argued that tuber formation is not simply regulated by gibberellins acting as the sole signal between above-ground and below-ground parts, since stolon tips are able to synthesize their own gibberellins, and the phenotype of phytochrome B-antisense plants cannot be explained only by altered levels of GAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358163
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  • 4
    Electronic Resource
    Electronic Resource
    Manipulation of tuber-size distribution of a potato crop (1990)
    Springer
    Potato research 33 (1990), S. 417-432 
    Details
    ISSN: 1871-4528
    Keywords: stolonization ; tuberization ; tuber bulking ; size grade ; stem number ; Solanum tuberosum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Tuber-size distribution is regulated by many diverse, interacting mechanisms and is therefore difficult to understand and manipulate. It is determined by plant density, number of stems per plant, number of tubers per stem, and yield. Seed size and plant number per unit area are easy to control, but stem number is affected by less controllable factors. Interactions between stems of different types are important for tuber-size distribution. The hormonal regulation of stolonization and tuberization is still unknown, but under the conditions of north-west Europe the process of tuber set (which is also poorly understood) makes a greater contribution to the final number of tubers than tuberization. The total yield is also relevant, because it affects both the average tuber size and its variation. Tubers on the same stem differ in timing, rate and duration of growth. The resulting hierarchy in sink strength is not consistent over time. Several mechanisms are suggested for this hierarchy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358019
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  • 5
    Electronic Resource
    Electronic Resource
    Possible mechanisms of size hierarchy among tubers on one stem of a potato (Solanum tuberosum L.) plant (1991)
    Springer
    Potato research 34 (1991), S. 187-203 
    Details
    ISSN: 1871-4528
    Keywords: size grade ; tuberization ; tuber bulking ; tuber-size distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The tubers on one potato stem vary greatly in size and their size distribution may be described by their number and their average size and its variation. These components are mutually and closely related. The variation is partly caused by stolon characteristics, including their date of initiation, position and size. Tuber size is therefore partly determined before tuberization. Tuber-specific factors, such as the position and activity of the tuber initial also play a role, but the date of initiation of an individual tuber is not crucial. Later on, during tuber bulking, the activity of enzymes involved in starch metabolism, the levels of hormones, the mineral composition and the turgor potential are associated with the tuber growth rate. The growth characteristics of individual tubers may also vary since they are exposed to different conditions because of differences in position or growing period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358041
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  • 6
    Electronic Resource
    Electronic Resource
    Changes in the microtubular cytoskeleton precede in vitro tuber formation in potato (1996)
    Springer
    Protoplasma 191 (1996), S. 46-54 
    Details
    ISSN: 1615-6102
    Keywords: Cytoskeleton ; Gibberellin ; In vitro culture ; Solanum tuberosum ; Tuber formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An in vitro system for tuber formation was used to study early morphological and cytological changes occurring during tuber formation in potatoes, with special emphasis on the orientation of the microtubular cytoskeleton, visualized immunocytochemically. Axillary buds from potato plants were cultured in the presence or absence of gibberellin (GA), resulting in either tuber formation (without GA) or shoot formation (GA added). Tuber formation in the absence of GA was highly synchronous in individual buds, enabling the dissection of various aspects of tuberization. Under both conditions, starch started to accumulate. In the absence of GA, starch levels rapidly increased, concomitantly with tuber formation, whereas it slightly decreased in the presence of GA. Up to 4 days, the cortical MTs in the cells were oriented perpendicular to the longitudinal axis of the developing buds. Under tuber-inducing conditions this orientation changed into a longitudinal one at day 5. This change preceded a change in the direction of cell expansion. In the presence of GA no such reorientation was observed, cells continued to grow longitudinally, and a stoloniferous shoot was formed. The cytoskeletal changes preceded the visible swelling of the buds, observed after day 5, demonstrating that the reorientation of the microtubular cytoskeleton is one of the earliest steps observed so far in tuber formation in potatoes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280824
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  • 7
    Electronic Resource
    Electronic Resource
    Similarity of QTLs detected for in vitro and greenhouse development of potato plants (1999)
    Springer
    Molecular breeding 5 (1999), S. 417-428 
    Details
    ISSN: 1572-9788
    Keywords: QTLs ; tuberization earliness ; in vitro conditions ; sugars in leaf exudate ; marker-assisted selection ; potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A relationship between quantitative trait loci (QTLs) detected for in vitro and greenhouse growing conditions was studied in a backcross population of 155 genotypes derived from a haploid potato (Solanum tuberosum) and a diploid wild species (S. berthaultii). Both plant height and tuberization earliness were characterized under two growing conditions. Main-effect QTLs and QTLs identified only through interaction were detected for each of the traits. For traits associated with plant height as well as for traits associated with early tuberization, the most significant QTL detected for greenhouse cultivated plants was also found when the population was grown in vitro. The most significant QTL for earliness of tuberization in vitro, which was located on chromosome 8, coincides with that detected for sucrose concentration in leaf exudate. The absence of a S. berthaultii allele was associated both with a higher amount of sucrose in the exudate and with earlier in vitro tuber formation. Epistasis was found to have a significant effect on all traits investigated. The QTL model that included main-effect QTLs and all significant interactions explained 83–88% of the total genetic variance for each of the developmental traits. The possibility of using an in vitro system combined with marker-assisted selection for preliminary selection of early tuberizing clones is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009686220862
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  • 8
    Electronic Resource
    Electronic Resource
    Genetics of self-compatibility in dihaploids of Solanum tuberosum L. 5. The S-genotype of tetraploid potato cultivar gineke (1978)
    Springer
    Euphytica 27 (1978), S. 385-388 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; incompatibility ; dihaploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Investigations of the genetics of self-compatibility and self-incompatibility in dihaploids and diploid derivatives from cv. Gineke revealed the presence of S 1, S2 and S 3 at the S-locus of Gineke and in addition an S 1-allele on a translocation. By means of a complete tester set involving the S-alleles S 1, S2 and S 3 (all from Gineke) and S 4 (from Black 4495) it was demonstrated that some Gineke dihaploids were compatible with all six testers. This indicated a fourth S-allele in Gineke, which differs from those in the tester series and was therefore assigned S 5. Additional evidence was obtained from an analysis of F1's from crosses of two S 5-bearing dihaploids and one of the testers. So the S-genotype of cv. Gineke was identified as S 1S2S3S5/S1, the second S 1 being the S-allele on a translocated fragment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043163
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  • 9
    Electronic Resource
    Electronic Resource
    Antisense suppression of a potato α-SNAP homologue leads to alterations in cellular development and assimilate distribution (2000)
    Springer
    Plant molecular biology 43 (2000), S. 473-482 
    Details
    ISSN: 1573-5028
    Keywords: α-SNAP ; antisense ; assimilate transport ; Solanum tuberosum ; vesicle trafficking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using the cDNA-AFLP method, we have isolated a transcript-derived fragment (TDF) which shows a differential expression pattern during tuber organogenesis of Solanum tuberosum L. The TDF was used to isolate a cDNA clone carrying a 1.5 kb insert and potentially coding for a 32.5 kDa peptide which, by homology, represents a potato homologue of an α-snap gene and has been designated Stsnap. Northern analysis showed that the Stsnap gene is expressed in actively dividing tissues throughout the potato plant. Analysis of genomic DNA from potato revealed that the Stsnap gene is likely to be a single-copy gene. The expression of antisense Stsnap cDNA under the control of the CaMV 35S promoter results in plants with an altered morphology such as curled leaves. Several of these transgenic lines also display cellular and developmental abnormalities with distinct changes in assimilate transport including accumulation of starch and soluble sugars in source leaves. We argue that these findings are consistent with the hypothetical function of the StSNAP gene product in vesicle targeting and fusion during plant development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006492205788
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of ethylene on the tuberization of potato (Solanum tuberosum) cuttings (1989)
    Springer
    Plant growth regulation 8 (1989), S. 31-39 
    Details
    ISSN: 1573-5087
    Keywords: Ethylene ; Solanum tuberosum ; stolon elongation ; tuber formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ethylene, applied as ethephon, inhibited the elongation of etiolated, axillary potato shoots cultured in vitro and it stimulated radial growth along the whole length of these shoots. The same phenomena were observed when ACC, the precursor of ethylene, was added to the medium, whereas silver ions reversed these effects. However, tuber formation in vitro was suppressed by ethephon. This indicates a dual role of ethylene in the induction of tuber formation in potatoes: it had a positive effect by blocking the elongation of stolons and it suppressed tuber initiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040914
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