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  • 1
    Electronic Resource
    Electronic Resource
    Totipotency and pluripotency of embryonic nuclei in the mouse (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 36 (1993), S. 276-278 
    Details
    ISSN: 1040-452X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080360230
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of anti-pig zona pellucida serum on fertilization in several mammals (1981)
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 231-239 
    Details
    ISSN: 0148-7280
    Keywords: inhibition of fertilization ; pig zonae pellucidae ; passive immunization ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effect of goat antiserum against isolated pig zonae pellucidae on fertilization in vivo was examined in the pig, cow, sheep, rabbit, rat, and mouse. As shown by indirect immunofluorescence, anti-pig zona serum reacted strongly with the zonae of pig, cow, sheep, and rabbit, but the reaction with the zonae of mouse and rat was weak. Passive immunization with anti-pig zona serum significantly, or completely, inhibited fertilization in all species. However, inhibition of fertilization was more pronounced in the pig, cow, sheep, rabbit, and mouse than in the rat. Inhibition of fertilization in the rabbit was also observed after passive immunization with antiserum absorbed with rabbit liver and kidney. All of the zonae recovered from the pig, cow, sheep, rat, and mouse after passive immunization with anti-pig zona serum exhibited strong fluorescence, regardless of the incidence of fertilization. It was concluded that the pig and other mammalian zonae pellucidae tested have tissue-specific antigens.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120040307
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of centrifugation of mouse eggs on their development in vitro and in vivo (1986)
    New York, NY : Wiley-Blackwell
    Gamete Research 15 (1986), S. 83-86 
    Details
    ISSN: 0148-7280
    Keywords: fertilized mouse eggs ; viability ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Fertilized mouse eggs were centrifuged at 5,000g, 10,000g, 15,000g, and 20,000g for 3 and 10 min or at 25,000g for 10 min. The pronuclei of centrifuged eggs became more distinctive than those of uncentrifuged eggs. The proportion of centrifuged and uncentrifuged eggs that developed to blastocysts was not significantly different. There was also no significant difference in the proportion of live fetuses obtained following the transfer of blastocysts developed from centrifuged and uncentrifuged eggs. This study demonstrated that there is no effect of centrifugation on the subsequent development of mouse eggs.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120150109
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  • 4
    Electronic Resource
    Electronic Resource
    Development of enucleated parthenogenones that received pronuclei or nuclei from fertilized mouse eggs (1988)
    New York, NY : Wiley-Blackwell
    Gamete Research 21 (1988), S. 151-155 
    Details
    ISSN: 0148-7280
    Keywords: nuclear transfer ; genome activation ; two-cell embryo ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: This study compares the developmental potential of enucleated mouse parthenogenones that received pronuclei from fertilized eggs with those that received nuclei from late two-cell embryos. The proportion of reconstituted parthenogenones, which received pronuclei at the one-cell stage, that developed to blastocysts in vitro and to live fetuses after transfer to recipients was significantly lower than that of reconstituted control eggs. However, the in vitro and in vivo developmental potential of reconstituted parthenogenones that received nuclei at the two-cell stage from fertilized late two-cell embryos was not different from that of reconstituted control eggs.These results were contrary to those reported previously by Mann and Lovell-Badge (1984), who showed that parthenogenetic eggs receiving pronuclei from fertilized eggs developed well both in vitro and in vivo.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120210205
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  • 5
    Electronic Resource
    Electronic Resource
    Altered sex ratio of live young after transfer of fast- and slow-developing mouse embryos (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 12 (1985), S. 301-304 
    Details
    ISSN: 0148-7280
    Keywords: sex ratio ; fast- and slow-developing embryos ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We cultured eight-cell mouse embryos to blastocyst stage, divided them into three groups according to the time of blastocoel formation, and transferred them separately into recipients. The proportion of live young from the fast-developing embryos was slightly high (49%) but not significantly different from those of other embryos (38% and 39%). However, the sex ratio of live young from the fast- and slow-developing embryos was significantly shifted toward the male (71%) and to the female (80%), respectively.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120120308
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of antibody to detergent solubilized zonae pellucidae on in vivo and in vitro fertilization in the mouse (1982)
    New York, NY : Wiley-Blackwell
    Gamete Research 6 (1982), S. 73-79 
    Details
    ISSN: 0148-7280
    Keywords: inhibition of fertilization ; mouse zonae pellucidae ; solubilized antigen ; purified antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: An antiserum was produced in one rabbit against mouse zonae pellucidae solubilized with 70 mM Na2SO3, 1% SDS, and 0.04 mM CuSO4. An IgG of zona antibody completely inhibited fertilization both in vitro and in vivo in the mouse. F(ab′)2 fragment obtained by pepsin digestion of IgG zona antibody inhibited fertilizability of eggs in vitro but did not inhibit fertilization in vivo after passive immunization.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120060109
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  • 7
    Electronic Resource
    Electronic Resource
    Parthenogenetic activation of mouse and rabbit eggs by electric stimulation in vitro (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 22 (1989), S. 277-283 
    Details
    ISSN: 0148-7280
    Keywords: mouse eggs ; rabbit eggs ; parthenogenetic activation ; electric stimulation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The present study was undertaken to determine conditions for parthenogenetic activation of mouse and rabbit eggs by electric stimulation in vitro. The cumulus-free eggs were submitted to square direct current pulses at output voltage of 1.0 to 2.5 kV/cm for 25 to 200 μsec. The best conditions for the activation of mouse eggs were 1.5 kV/cm for 100 μsec, in which 78% of eggs were activated, 32% of which developed to blastocysts in vitro. When the nonelectric solution (0.3M mannitol) was used for electric stimulation, the activation rate was quite low (16%). Optimal conditions for activation of rabbit eggs were 1.5 kV/cm for 200 μsec, in which 77% of eggs were activated, 25% of which developed to blastocysts. Unlike mouse eggs, rabbit eggs frequently had three pronuclei after electric stimulation.It is clearly shown that electric stimulus can induce parthenogenetic activation of the mouse and rabbit eggs in vitro.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120220305
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  • 8
    Electronic Resource
    Electronic Resource
    Development of single blastomeres from four- and eight-cell mouse embryos fused into the enucleated half of a two-cell embryo (1989)
    New York, NY : Wiley-Blackwell
    Gamete Research 22 (1989), S. 427-434 
    Details
    ISSN: 0148-7280
    Keywords: nuclear transfer ; cell number ; outgrowth ; live young ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Single blastomeres from four- and eight-cell mouse embryos were fused into the enucleated halves of two-cell embryos, and the ability of these reconstituted embryos to develop in vitro and in vivo was examined. The proportion of these reconstituted embryos developing to blastocysts was 74% (60/81) when four-cell embryo blastomeres were used as nuclei donors and 31% (57/182) when eight-cell embryo blastomeres were used. Eight complete sets of the quadruplet-reconstituted embryos developed to blastocysts, and five live young (9%, 5/57) were obtained after transfer; however, none of the live young were clones. Although when using blastomeres from eight-cell embryos no complete set of eight developed to blastocysts, sextuplets were obtained. The blastocysts, however, failed to produce live young after transfer. In assessing the outgrowths, it was found that 43% of those derived from reconstituted embryos using blastomeres from four-cell embryos had an inner cell mass (ICM); however, outgrowths derived from reconstituted embryos using blastomeres from eight-cell embryos lacked an ICM. These results suggest that the genomes of four- and eight-cell nuclei introduced into the enucleated halves of two-cell embryos are reversed to support the development of the reconstituted embryo.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120220408
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  • 9
    Electronic Resource
    Electronic Resource
    Electrofusion for the pronuclear transplantation of mouse eggs (1987)
    New York, NY : Wiley-Blackwell
    Gamete Research 17 (1987), S. 15-20 
    Details
    ISSN: 0148-7280
    Keywords: nuclear transplantation ; electrofusion ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The present study was undertaken to determine the efficiency of HVJ treatment and electrofusion for pronuclear transplantation in the mouse. The output voltage and duration of the pulses were fixed to 200 μsec at 10 V or to 150 μsec at 15 V for electrofusion, because the maximum rates of blastomere fusion of 2-cell embryos and development of fused embryos in vitro were obtained under these conditions. Although the proportion of eggs with fused karyoplast (78%) and the fused eggs developed to morulae or blastocysts (67%) was significantly lower than those obtained after HVJ treatment (94% and 94%), the proportion of pregnant recipients and young obtained after treatment of fused eggs was not significantly different between these two procedures.It is advised that electrofusion can be used as a fusogenic procedure for pronuclear transplantation in the mouse in some cases where HVJ cannot be applied.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120170103
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  • 10
    Electronic Resource
    Electronic Resource
    Inhibition of fertilization in cattle by passive immunization with anti-zona pellucida serum (1981)
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 133-138 
    Details
    ISSN: 0148-7280
    Keywords: inhibition of fertilization ; bovine zonae pellucidae ; passive immunization ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Passive immunization with antiserum prepared against isolated bovine zonae pellucidae inhibited fertilization in the cow. The minimum dosage of antiserum (titer 27 by immunofluorescence) required for complete inhibition was 2 ml/kg of body weight.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120040207
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