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  • Articles  (3)
  • Life Sciences (general)  (1)
  • Life and Medical Sciences  (1)
  • bottled water  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Bottled-Water Quality in Metropolitan Mexico City (1999)
    Springer
    Water, air & soil pollution 113 (1999), S. 217-226 
    Details
    ISSN: 1573-2932
    Keywords: bottled water ; bacteriological quality and physico-chemicalquality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Bacteriological and physico-chemical parameters of 265 samples from 39 brands sold in 5-gallon plastic and glass bottles and 2-5 L plastic containers were analyzed to determine the quality of bottled water distributed in Mexico City. Tests included fecal and total coliform counts, alkalinity, total hardness, chloride, calcium and magnesium concentrations, pH and conductivity. Correlation and cluster analyses and ANOVA were carried out, and a comparison made of the averages with the maximum permissible levels established in the Official Mexican Norms. Concerning the investigated parameters no differences (p 〉 0.05) between the brands were found. Physico-chemical parameters were studied and all the samples were within the permissible limits. Most samples taken from the 5-gallon containers exceeded the maximum bacteriological limits. It was concluded that the bacteriological quality of the brands studied was extremely variable. Appropriate sanitary measures, should be established to control this aspect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005088011681
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of CHS4 (CAL2), a Gene of Saccharomyces cerevisiae Involved in Chitin Biosynthesis and Allelic to SKT5 and CSD4 (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 795-807 
    Details
    ISSN: 0749-503X
    Keywords: chitin synthase III ; morphogenesis ; Calcofluor resistance ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have cloned CHS4, a gene that complements the resistance to Calcofluor of the Saccharomyces cerevisiae cal2 mutant. We show that CHS4 is allelic to the previously described SKT5 and CSD4 genes. CHS4 encodes a 696 amino acids protein with no potential transmembrane domain. chs4-null mutants are resistant to Calcofluor white and exhibit a considerable reduction in cell wall chitin and in chitin synthase III (CSIII) activity. Biochemical characterization of chitin synthase III from these null mutants indicates that the defect is due to a reduced Vmax of the enzyme. This defect can be overcome in vitro by trypsin treatment of the membrane preparations. Chs4p does not act as a transcriptional or translational regulator of CHS3, the gene coding for the catalytic subunit of CSIII activity, and we therefore propose that Chs4p would be an essential component of the CSIII complex, acting as a post-translational regulator of this activity. In addition to the chitin defect, the chs4 mutant shows a severe defect in mating. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of cwl1+, a gene from Schizosaccharomyces pombe whose overexpression causes cell lysis (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 12 (1996), S. 983-990 
    Details
    ISSN: 0749-503X
    Keywords: fission yeast ; dominant genetics ; cell wall regulation ; Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: From a Schizosaccharomyces pombe genomic library we have isolated the gene cwl1+ that causes cell lysis when it is overexpressed in the absence of an osmotic stabilizer. Southern hybridization showed that cwl1+ exists as a single copy in the S. pombe genome. The cwl1+ gene nucleotide sequence revealed a putative open reading frame of 924 bp encoding a polypeptide of 308 amino acids with a calculated Mr of 27 000. The cwl1+ DNA hybridizes to a major RNA transcript of 1·5 kb whose 5′ end maps at a position 452 bp upstream from the predicted translation start. Comparison of the amino acid sequence with those included in the current databases, showed no significant similarity to any known sequences. Cells overexpressing the cwl1+ gene under the control of the S. pombe nmt inducible promoter displayed a reduced cell wall content, were unable to separate after division and lysed drastically in the absence of osmotic stabilizer. Disruption of the cwl1+ gene caused no noticeable phenotype. The sequence has been deposited in the EMBL data library under Accession Number X9445.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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