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  • Life Sciences (General)  (6)
  • Protocols  (1)
  • Right whale  (1)
  • 1
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    Mechanics of the right whale mandible : full scale testing and finite element analysis (2009)
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    Publication Date: 2022-05-25
    Description: Author Posting. © Elsevier B.V., 2009. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Experimental Marine Biology and Ecology 374 (2009): 93-103, doi:10.1016/j.jembe.2009.03.012.
    Description: In an effort to better understand the mechanics of ship-whale collision and to reduce the associated mortality of the critically endangered North Atlantic right whale, a comprehensive biomechanical study has been conducted by the Woods Hole Oceanographic Institution and the University of New Hampshire. The goal of the study is to develop a numerical modeling tool to predict the forces and stresses during impact and thereby the resulting mortality risk to whales from ship strikes. Based on post-mortem examinations, jaw fracture was chosen as a fatal endpoint for the whales hit by a vessel. In this paper we investigate the overall mechanical behavior of a right whale mandible under transverse loading and develop a finite element analysis model of the bone. The equivalent elastic modulus of the cortical component of right whale mandible is found by comparing full-scale bending tests with the results of numerical modeling. The finite element model of the mandible can be used in conjunction with a vessel-whale collision event model to predict bone fracture for various ship strike scenarios.
    Description: Funding for this work was provided by the National Science Foundation (Graduate Research Fellowship Program, Campbell-Malone), the National Oceanic and Atmospheric Administration (Right Whale Grants Program, award number NA04NMF4720402), and the Woods Hole Oceanographic Institution Ocean Life Institute.
    Keywords: Right whale ; Mandible ; Mechanical testing ; Finite element analysis
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
    Format: application/pdf
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  • 2
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    Protocols for calibrating multibeam sonar (2008)
    Acoustical Society of America
    Details
    Publication Date: 2022-05-25
    Description: Author Posting. © Acoustical Society of America, 2005. This article is posted here by permission of Acoustical Society of America for personal use, not for redistribution. The definitive version was published in Journal of the Acoustical Society of America 117 (2005): 2013-2027, doi:10.1121/1.1869073.
    Description: Development of protocols for calibrating multibeam sonar by means of the standard-target method is documented. Particular systems used in the development work included three that provide the water-column signals, namely the SIMRAD SM2000/90- and 200-kHz sonars and RESON SeaBat 8101 sonar, with operating frequency of 240 kHz. Two facilities were instrumented specifically for the work: a sea well at the Woods Hole Oceanographic Institution and a large, indoor freshwater tank at the University of New Hampshire. Methods for measuring the transfer characteristics of each sonar, with transducers attached, are described and illustrated with measurement results. The principal results, however, are the protocols themselves. These are elaborated for positioning the target, choosing the receiver gain function, quantifying the system stability, mapping the directionality in the plane of the receiving array and in the plane normal to the central axis, measuring the directionality of individual beams, and measuring the nearfield response. General preparations for calibrating multibeam sonars and a method for measuring the receiver response electronically are outlined. Advantages of multibeam sonar calibration and outstanding problems, such as that of validation of the performance of multibeam sonars as configured for use, are mentioned.
    Description: Support by the National Science Foundation through Award No. OCE-0002664, NOAA through Grant No. NA97OG0241, and the Cooperative Institute for Climate and Ocean Research (CICOR) through NOAA Contract No. NA17RJ1223 is acknowledged.
    Keywords: Sonar detection ; Sonar target recognition ; Underwater sound ; Calibration ; Array signal processing ; Acoustic transducer arrays ; Protocols
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 3
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    Isolation and characterization of a novel gene sfig in rat skeletal muscle up-regulated by spaceflight (STS-90) (2003)
    In:  Other Sources
    Details
    Publication Date: 2011-08-24
    Description: We obtained the skeletal muscle of rats exposed to weightless conditions during a 16-day-spaceflight (STS-90). By using a differential display technique, we identified 6 up-regulated and 3 down-regulated genes in the gastrocnemius muscle of the spaceflight rats, as compared to the ground control. The up-regulated genes included those coding Casitas B-lineage lymphoma-b, insulin growth factor binding protein-1, titin and mitochondrial gene 16 S rRNA and two novel genes (function unknown). The down-regulated genes included those encoding RNA polymerase II elongation factor-like protein, NADH dehydrogenase and one novel gene (function unknown). In the present study, we isolated and characterized one of two novel muscle genes that were remarkably up-regulated by spaceflight. The deduced amino acid sequence of the spaceflight-induced gene (sfig) comprises 86 amino acid residues and is well conserved from Drosophila to Homo sapiens. A putative leucine-zipper structure located at the N-terminal region of sfig suggests that this gene may encode a transcription factor. The up-regulated expression of this gene, confirmed by Northern blot analysis, was observed not only in the muscles of spaceflight rats but also in the muscles of tail-suspended rats, especially in the early stage of tail-suspension when gastrocnemius muscle atrophy initiated. The gene was predominantly expressed in the kidney, liver, small intestine and heart. When rat myoblastic L6 cells were grown to 100% confluence in the cell culture system, the expression of sfig was detected regardless of the cell differentiation state. These results suggest that spaceflight has many genetic effects on rat skeletal muscle.
    Keywords: Life Sciences (General)
    Type: The journal of medical investigation : JMI (ISSN 1343-1420); Volume 50; 1-2; 39-47
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  • 4
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    Effects of microgravity on myogenic factor expressions during postnatal development of rat skeletal muscle (2002)
    In:  Other Sources
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    Publication Date: 2011-08-24
    Description: To clarify the role of gravity in the postnatal development of skeletal muscle, we exposed neonatal rats at 7 days of age to microgravity. After 16 days of spaceflight, tibialis anterior, plantaris, medial gastrocnemius, and soleus muscles were removed from the hindlimb musculature and examined for the expression of MyoD-family transcription factors such as MyoD, myogenin, and MRF4. For this purpose, we established a unique semiquantitative method, based on RT-PCR, using specific primers tagged with infrared fluorescence. The relative expression of MyoD in the tibialis anterior and plantaris muscles and that of myogenin in the plantaris and soleus muscles were significantly reduced (P 〈 0.001) in the flight animals. In contrast, MRF4 expression was not changed in any muscle. These results suggest that MyoD and myogenin, but not MRF4, are sensitive to gravity-related stimuli in some skeletal muscles during postnatal development.
    Keywords: Life Sciences (General)
    Type: Journal of applied physiology (Bethesda, Md. : 1985) (ISSN 8750-7587); Volume 92; 5; 1936-42
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  • 5
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    Functional and cellular adaptations of rodent skeletal muscle to weightlessness (1995)
    In:  CASI
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    Publication Date: 2019-06-28
    Description: This paper describes the affects of microgravity upon three key cellular levels (functional, protein, and mRNA) that are linked to one another. It is clear that at each of these levels, microgravity produces rapid and substantial alterations. One of the key challenges facing the life science community is the development of effective countermeasures that prevent the loss of muscle function as described in this paper. The development of optimal countermeasures, however, awaits a clearer understanding of events occurring at the levels of transcription, translation, and degradation.
    Keywords: Life Sciences (General)
    Type: Journal of Gravitational Physiology, Volume 2, No. 1; 39-42
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  • 6
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    Cellular and molecular responses to increased skeletal muscle loading after irradiation (2002)
    In:  Other Sources
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    Publication Date: 2019-07-13
    Description: Irradiation of rat skeletal muscles before increased loading has been shown to prevent compensatory hypertrophy for periods of up to 4 wk, possibly by preventing satellite cells from proliferating and providing new myonuclei. Recent work suggested that stem cell populations exist that might allow irradiated muscles to eventually hypertrophy over time. We report that irradiation essentially prevented hypertrophy in rat muscles subjected to 3 mo of functional overload (OL-Ir). The time course and magnitude of changes in cellular and molecular markers of anabolic and myogenic responses were similar in the OL-Ir and the contralateral nonirradiated, overloaded (OL) muscles for the first 3-7 days. These markers then returned to control levels in OL-Ir muscles while remaining elevated in OL muscles. The number of myonuclei and amount of DNA were increased markedly in OL but not OL-Ir muscles. Thus it appears that stem cells were not added to the irradiated muscles in this time period. These data are consistent with the theory that the addition of new myonuclei may be required for compensatory hypertrophy in the rat.
    Keywords: Life Sciences (General)
    Type: American journal of physiology. Cell physiology (ISSN 0363-6143); 283; 4; C1182-95
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  • 7
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    Spacelab Life Sciences Research Panel (2000)
    In:  CASI
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    Publication Date: 2019-08-28
    Description: This document describes some of the life sciences research that was conducted on Spacelab missions. Dr. Larry Young, Director of the National Space Biomedical Research Institute, provides an overview of the Life Sciences Spacelabs.
    Keywords: Life Sciences (General)
    Type: The Spacelab Accomplishments Forum; 115-170; NASA/CP-2000-210332
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  • 8
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    Skeletal muscle plasticity: cellular and molecular responses to altered physical activity paradigms (2002)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: The goal of this article is to examine our current understanding of the chain of events known to be involved in the adaptive process whereby specific genes and their protein products undergo altered expression; specifically, skeletal muscle adaptation in response to altered loading states will be discussed, with a special focus on the regulation of the contractile protein, myosin heavy chain gene expression. This protein, which is both an important structural and regulatory protein comprising the contractile apparatus, can be expressed as different isoforms, thereby having an impact on the functional diversity of the muscle. Because the regulation of the myosin gene family is under the control of a complex set of processes including, but not limited to, activity, hormonal, and metabolic factors, this protein will serve as a cellular "marker" for studies of muscle plasticity in response to various mechanical perturbations in which the quantity and type of myosin isoform, along with other important cellular proteins, are altered in expression.
    Keywords: Life Sciences (General)
    Type: American journal of physical medicine & rehabilitation / Association of Academic Physiatrists (ISSN 0894-9115); 81; 11 Suppl; S40-51
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