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  • Articles  (3)
  • Data
  • Industrial Chemistry and Chemical Engineering  (1)
  • MolluscicidalBacillus toxin  (1)
  • migration  (1)
  • Process Engineering, Biotechnology, Nutrition Technology  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Fermentation and toxin studies of the molluscicidal strains ofBacillus brevis (1994)
    Springer
    Journal of industrial microbiology and biotechnology 13 (1994), S. 112-119 
    Details
    ISSN: 1476-5535
    Keywords: MolluscicidalBacillus toxin ; Bacillus brevis ; Biomphalaria glabrata ; Biocontrol of snails ; Antioxidant preservation of toxin ; Secondary fermentation factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Strain SS86-4 was one of 40Bacillus brevis strains shown to be molluscicidal to the schistosomiasis snail vectorBiomphalaria glabrata. When grown in mB4 medium in 2-L fermentors, SS86-4 was molluscicidal only if fructose or phenylalanine was present in the medium. This is reminiscent of secondary fermentation factor effects, in this case an antioxidant effect. In vivo proteases also were capable of reducing molluscicidal activity. The molluscicidal toxin has an LC50 of 1 μg toxin protein ml−1 (approx. 1 p.p.m.) and may be described as a small proteinaceous, heat-stable, oxygen-sensitive entity associated with the particulate portion of the cell wall fraction ofB. brevis that is formed prior to sporulation. Initial information indicates that its HPLC signature shows major peaks at 148.37 and 163.96 s and consists of two bands of approximately 5.3 kDa and 8.7 kDa on PAGE gel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01584108
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  • 2
    Electronic Resource
    Electronic Resource
    Perimeter Exploration of Linear Chains of Circles (1995)
    Weinheim : Wiley-Blackwell
    Particle and Particle Systems Characterization 12 (1995), S. 257-263 
    Details
    ISSN: 0934-0866
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Perimeter data, obtained from structured walks performed upon straight chains of circles and displayed in Richardson plots, reveal two distinct classes of discontinuities: textural and structural. Textural discontinuities contain information about the surface features of the profile, while structural discontinuities reveal the profile's overall shape. The origins of both types of discontinuities have easy explanations. The highly predictable behavior of the structural discontinuities serves as the basis of a newly proposed method of analysis for perimeter data. The number of structural discontinuities is equal to N, the number of circles in the chain; furthermore, the value of the step size \documentclass{article}\pagestyle{empty}\begin{document}$ \underline {\lambda} $\end{document} * associated with the first structural discontinuity is inversely related to N. These properties seem is be retained when the circles slightly intersect and also when the circles vary in diameter. Analysis of the structural discontinuities may provide a new method suitable for automated examination of chain-like agglomerate particles.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ppsc.19950120508
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  • 3
    Electronic Resource
    Electronic Resource
    Osteoblast migration on poly(α-hydroxy esters) (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 50 (1996), S. 443-451 
    Details
    ISSN: 0006-3592
    Keywords: osteoblast ; migration ; poly(αhydroxy esters) ; poly(DL-lactic-co-glycolic acid) ; PLGA ; biodegradable polymers ; tissue engineering ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We investigated the migration of rat calvaria osteoblast populations on poly(α-hydroxy ester) films for up to 14 days to determine effects of substrate composition and culture conditions on the migratory characteristics of osteoblasts. Initial osteoblast culture conditions included cell colonies formed by seeding a high (84,000 cells/cm2) or low (42,000 cells/cm2) density of isolated osteoblasts on the polymer films, and bone tissue cultures formed by plating bone chips directly on the substrates. High density osteoblast colonies cultured and allowed to migrate and proliferate radially on 85:15 poly(DL-lactic-co-glycolic acid) (PLGA) films, 75:25 PLGA films, and tissue culture polystyrene controls demonstrated that the copolymer ratio in the polymer films did not affect the rate of increase in substrate surface area (or culture area) covered by the growing cell colony. However, the rate of increase in culture area was dependent on the initial osteoblast seeding density. Initial cell colonies formed with a lower osteoblast seeding density on 75:25 PLGA resulted in a lower rate of increase in culture area, specifically 4.9 ± 0.3 mm2/day, versus 14.1 ± 0.7 mm2/day for colonies seeded with a higher density of cells on the same polymer films. The proliferation rate for osteoblasts in the high and low density seeded osteoblast colonies did not differ, whereas the proliferation rate for the osteoblasts arising from the bone chips was lower than either of these isolated cell colonies. Confocal and light microscopy revealed that the osteoblast migration occurred as a monolayer of individual osteoblasts and not a calcified tissue front. These results demonstrated that cell seeding conditions strongly affect the rates of osteoblast migration and proliferation on biodegradable poly(α-hydroxy esters). © 1996 John Wiley & Sons, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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