Publication Date:
1991-07-19
Description:
Mycosis fungoides, a rare form of cutaneous T cell leukemia/lymphoma, is suspected of having a viral etiology on the basis of certain similarities to adult T cell leukemia, which is associated with human T cell leukemia/lymphoma virus type I (HTLV-I) infection. Cell lines were established from peripheral blood mononuclear cells (PBMC) of an HTLV-I-seronegative patient with mycosis fungoides. DNA hybridization analysis revealed the presence of HTLV-I-related sequences with unusual restriction endonuclease sites. Sequence analysis of subcloned fragments demonstrated the presence of a monoclonally integrated provirus with a 5.5-kilobase deletion involving large regions of gag and env and all of pol. Additional evidence for the presence of deleted proviruses was found by polymerase chain reaction (PCR) amplification of DNA from cutaneous lesions of five other HTLV-I-seronegative patients. The findings suggest that HTLV-I infection may be involved in the etiology of at least certain cases of mycosis fungoides.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hall, W W -- Liu, C R -- Schneewind, O -- Takahashi, H -- Kaplan, M H -- Roupe, G -- Vahlne, A -- CA51012-01A1/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1991 Jul 19;253(5017):317-20.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Infectious Disease, North Shore University Hospital-Cornell University Medical College, Manhasset, NY 11030.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1857968" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
*Chromosome Deletion
;
DNA, Neoplasm/genetics/isolation & purification
;
DNA, Viral/genetics/isolation & purification
;
*Genes, Viral
;
Human T-lymphotropic virus 1/genetics/*isolation & purification
;
Humans
;
Lymphocytes/*microbiology
;
Molecular Sequence Data
;
Mycosis Fungoides/blood/*microbiology
;
Oligonucleotide Probes
;
Polymerase Chain Reaction
;
Proviruses/genetics/*isolation & purification
;
Restriction Mapping
;
Sequence Homology, Nucleic Acid
;
Skin/*microbiology
;
Skin Neoplasms/blood/*microbiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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