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  • 1
    Electronic Resource
    Electronic Resource
    UV effects on photosynthesis, proteins and pigmentation in the flagellate Euglena gracilis: Biochemical and spectroscopic observations
    Amsterdam : Elsevier
    Biochemical Systematics and Ecology 20 (1992), S. 485-492 
    Details
    ISSN: 0305-1978
    Keywords: Eugelena ; FPLC ; UV radiation ; absorption spectroscopy ; chlorophyll ; fluorescence spectroscopy ; photosynthesis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0305-1978(92)90002-U
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  • 2
    Electronic Resource
    Electronic Resource
    Microsatellite analysis of maternal half-sib families of Quercus robur, pedunculate oak: II. inferring the number of pollen donors from the offspring (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 858-865 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oak ; Quercus ; Relatedness ; Linkage disequilibrium ; Microsatellites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We present an approach to infer the number of pollen donors directly from genotype data of open- pollinated progeny of Quercus robur (pedunculate oak), a highly outcrossing tree species. The approach is based on closely linked, highly polymorphic codominant microsatellite markers. Initially the close linkages between three previously mapped microsatellite loci were confirmed by studies of linkage disequilibrium (LD). Then an approach to track the pollen donors contributing to maternal half-sib families (open-pollinated families) was developed by analysing haplotype arrays of closely linked microsatellite markers transmitted from the fathers to the progeny. Simulated data of five linked microsatellite loci segregating in eight open-pollinated families were used to study the relationship between the number of paternal chromosomes detected by this ”haplotype approach” and the number of diploid fathers contributing to the families. The results showed that the number of diploid pollen donors can be expressed as an exponential function of the number of paternal chromosomes inferred from the progeny. The 95% confidence interval of this regression function is used to determine the minimum number of fathers contributing to a genotyped open-pollinated family of Quercus robur. Finally this open-pollinated family is used to demonstrate the resolution obtained with the ”haplotype approach”. Six independent microsatellite loci were used to study relatedness among all pairs of pollen gametes that share a haplotype of three linked markers. The results suggest that the majority of such gametes are identical by descent from the same father. The ”haplotype approach” presented here can be used to monitor the number of contributing pollen donors in commercial seedlot samples from oak or any other outcrossing tree species for which closely linked, highly polymorphic, codominant genetic markers are available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051362
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  • 3
    Electronic Resource
    Electronic Resource
    An estimation of the genome length of maritime pine (Pinus pinaster Ait.) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 289-292 
    Details
    ISSN: 1432-2242
    Keywords: Genome ; Linkage ; Pine ; Gymnosperm ; Two-dimensional electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genome length, in units of Morgans or centimorgans, is a fundamental feature of a species. It can be calculated from a complete linkage map. However, the genome size can be estimated with partial linkage data. Using linkage data obtained by the analysis of a two-dimensional electrophoresis of the proteins contained in an haploid organ, the megagametophyte, we suggest an estimation and a confidence interval of the genome length of a gymnosperm, the maritime pine (Pinus pinaster Ait.). The results indicate an important gap between the physical and the genetic maps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223634
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  • 4
    Electronic Resource
    Electronic Resource
    Estimation and test for linkage between markers: a comparison of lod score and χ 2 test in a linkage study of maritime pine (Pinus pinaster Ait.) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 293-297 
    Details
    ISSN: 1432-2242
    Keywords: Linkage test ; Lod score ; Pine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first step in the construction of a linkage map involves the estimation and test for linkage between all possible pairs of markers. The lod score method is used in many linkage studies for the latter purpose. In contrast with classical statistical tests, this method does not rely on the choice of a first-type error level. We thus provide a comparison between the lod score and a χ 2 test on linkage data from a gymnosperm, the maritime pine. The lod score appears to be a very conservative test with the usual thresholds. Its severity depends on the type of data used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223635
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  • 5
    Electronic Resource
    Electronic Resource
    Seed-protein variation in maritime pine (Pinus pinaster Ait.) revealed by two-dimensional electrophoresis: genetic determinism and construction of a linkage map (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 521-528 
    Details
    ISSN: 1432-2242
    Keywords: Pine ; Two-dimensional electrophoresis ; Haploid megagametophyte ; Linkage map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Proteins from haploid megagametophytes from 18 trees were studied by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). A total of 222 seeds, an average of 12 per tree, were analysed individually. 150 protein spots appeared to segregate on the polyacrylamide gels in at least tree. Genetic interpretations were made to define the number of loci responsible for the presence versus absence, staining differences or position variation of the segregating spots. The complete covariation observed between some spots could be the result of either the separation of a single gene product into two or more constituents, very close linkage, or the action of a pleiotropic gene. Human genetics techniques were used to map the 84 putative loci detected. Sixty-five loci were organised in 17 linkage groups, whereas 19 remained unlinked.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220908
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