Publication Date:
1993-12-10
Description:
The function and regulation of DNA methylation in eukaryotes remain unclear. Genes affecting methylation were identified in the fungus Neurospora crassa. A mutation in one gene, dim-2, resulted in the loss of all detectable DNA methylation. Abnormal segregation of the methylation defects in crosses led to the discovery that the methylation mutants frequently generate strains with extra chromosomes or chromosomal parts. Starvation for S-adenosylmethionine, the presumed methyl group donor for DNA methylation, also produced aneuploidy. These results suggest that DNA methylation plays a role in the normal control of chromosome behavior.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Foss, H M -- Roberts, C J -- Claeys, K M -- Selker, E U -- GM-35690/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1993 Dec 10;262(5140):1737-41.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Molecular Biology, University of Oregon, Eugene 97403.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7505062" target="_blank"〉PubMed〈/a〉
Keywords:
5-Methylcytosine
;
Aneuploidy
;
Azacitidine/pharmacology
;
Blotting, Southern
;
Chromosomes, Fungal/*metabolism
;
Crosses, Genetic
;
Cytosine/*analogs & derivatives/analysis
;
DNA, Fungal/chemistry/*metabolism
;
Genes, Fungal
;
Genetic Complementation Test
;
Methionine/metabolism
;
Methylation
;
Mutation
;
Neurospora crassa/*genetics/growth & development
;
Phenotype
;
S-Adenosylmethionine/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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