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  • 1
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    Introducing genetic markers to identify and distinguish five species of Cyprinidae in the Caspian Sea using PCR-RFLP (2018)
    Details
    Publication Date: 2021-05-19
    Description: Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus fish kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500[1.1 tubes and amplified using PCR method with a pair of primers with cytochrom b gene sequence of Kora volba. For RFLP analysis, PCR products of cytochorome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I. Hha I , Hine II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinf I, Hha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
    Description: Published
    Keywords: Cyprinidae ; PCR-RFLP ; Barbus capito ; Bream Abramis brama ; Aspius aspius ; Rutilus rutilus caspius ; Aspius aspius ; Polyacrylamide ; Molecular Marke
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.49-58
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  • 2
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    Introducing genetic markers to identify and distinguish five species of Cyprinidae in the Caspian Sea using PCR-RFLP (2018)
    Details
    Publication Date: 2021-05-19
    Description: Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus frisii kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500gl tubes and amplified using PCR method with a pair of primers with cytochrome b gene sequence of Kora volba. For RFLP analysis, PCR products of cytochrome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I, Hhal, Hinc II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinfl, flha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
    Description: Published
    Keywords: Genetic ; Species ; Cyprinidae ; PCR-RFLP ; Molecular Marker ; Rutilus frisii kutum ; Specimens ; Barbus capito ; Bream Abramis brama ; Aspius aspius ; Rutilus rutilus ; DNA ; Enzymes
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.49-58
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  • 3
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    Recognation of fishes in rivers and basin of Qom (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Geographical ; Fish ; Samples ; Species ; Fisheries ; Electroshoker ; Cyprinidae ; Balitoridae ; Cyprinodontidae ; Poecilidae ; Aquatic ; Recognation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 67pp.
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  • 4
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    Recognition of fishes in rivers and basin of Qom (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25143 | 18721 | 2018-08-26 16:50:02 | 25143 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: This study was carried out on rivers, basins & qanat of Qom region during the 1376-1378and Fish samples were taken from 2 permanent rivers (Qomrood ,Ghara_chag)& from some seasonal ones (Tagharood, Zavarian, Vesva, Biraghan). Also local fishes was followed in 100 qanats in the region and Random fish sampling was done in permanent river extension out of province. This study aimed to recognize different fish species in the province water reservoir and estimating the fisheries potential in the province. For fish sampling nets such as mashk, salik, hook and hand tailored were used. Electroshoker was not used at all. Results of fish recognition showed that fish samples belong to 12 species & 4 family of "cyprinidae", "Balitoridae", "cyprinodontidae" and "poecilidae".The qanat fishes of the montain (kahak, Khagestan) and pastora area (Ghanavat,jafarabad) included by two families of "cyprinid", "Balitoridea".In some qanat which were connected to rivers more species of fish and anotherAquatic animal were observed & sampled.At the and map of geographical distribution of local fishes was planned.
    Keywords: Biology ; Iran ; Qom province ; Qomrood ; Ghara_chag ; Tagharood ; Fish ; Samples ; Species ; Fisheries ; Electroshoker ; Cyprinidae ; Balitoridae ; Cyprinodontidae ; Poecilidae ; Aquatic ; Recognation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 67
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  • 5
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    Unknown
    Introducing genetic markers to identify and distinguish five species of Cyprinidae in the Caspian Sea using PCR-RFLP (2021)
    In:  http://aquaticcommons.org/id/eprint/24743 | 18721 | 2018-08-04 17:09:13 | 24743 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Cyprinids are the main and most significant bony fishes found in the Caspian Sea. In this study, 15 specimens of Rutilus fish kutum (Kamensky, 1901), 10 specimens of Barbus capito (Gueldenstaedtii, 1772), 10 specimens of Bream Abramis brama (Linnaeus, 1785), 10 specimens of Redlip, Aspius aspius, and 15 specimens of Kora volba, Rutilus rutilus caspius, were collected from commercial catch stations. DNA from all specimens was extracted using the phenol-chloroform method in 500[1.1 tubes and amplified using PCR method with a pair of primers with cytochrom b gene sequence of Kora volba. For RFLP analysis, PCR products of cytochorome gene b (1117 bp) from each species were digested with five restriction enzymes under suitable conditions of incubation. DNA bands were visualized by gel electrophoresis (polyacrylamide) and staining with silver nitrate. Five enzyme Rsa I, Hinf I. Hha I , Hine II, and Mbo I showed polymorphism. Genotypes obtained from digestion of enzymes Rsa I, Hinf I, Hha I showed haplotypes AAAAA for Barbus capito, BBBB for R. rutilus caspius, CCCC for Bream Abramis brama, DDDD for Aspius aspius and EEEE for R. frisii kutum. Each of these haplotypes serves as a genetic marker for the species and is of significant importance in distinguishing them.
    Keywords: Biology ; Cyprinidae ; Molecular Marke ; PCR-RFLP ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 49-58
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