Publication Date:
2010-12-24
Description:
Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental stages. We identified 111,195 new elements, including thousands of genes, coding and non-coding transcripts, exons, splicing and editing events, and inferred protein isoforms that previously eluded discovery using established experimental, prediction and conservation-based approaches. These data substantially expand the number of known transcribed elements in the Drosophila genome and provide a high-resolution view of transcriptome dynamics throughout development.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075879/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3075879/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Graveley, Brenton R -- Brooks, Angela N -- Carlson, Joseph W -- Duff, Michael O -- Landolin, Jane M -- Yang, Li -- Artieri, Carlo G -- van Baren, Marijke J -- Boley, Nathan -- Booth, Benjamin W -- Brown, James B -- Cherbas, Lucy -- Davis, Carrie A -- Dobin, Alex -- Li, Renhua -- Lin, Wei -- Malone, John H -- Mattiuzzo, Nicolas R -- Miller, David -- Sturgill, David -- Tuch, Brian B -- Zaleski, Chris -- Zhang, Dayu -- Blanchette, Marco -- Dudoit, Sandrine -- Eads, Brian -- Green, Richard E -- Hammonds, Ann -- Jiang, Lichun -- Kapranov, Phil -- Langton, Laura -- Perrimon, Norbert -- Sandler, Jeremy E -- Wan, Kenneth H -- Willingham, Aarron -- Zhang, Yu -- Zou, Yi -- Andrews, Justen -- Bickel, Peter J -- Brenner, Steven E -- Brent, Michael R -- Cherbas, Peter -- Gingeras, Thomas R -- Hoskins, Roger A -- Kaufman, Thomas C -- Oliver, Brian -- Celniker, Susan E -- U01 HB004271/HB/NHLBI NIH HHS/ -- U01 HG004271/HG/NHGRI NIH HHS/ -- U01 HG004271-01/HG/NHGRI NIH HHS/ -- ZIA DK015600-14/Intramural NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2011 Mar 24;471(7339):473-9. doi: 10.1038/nature09715. Epub 2010 Dec 22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics and Developmental Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, Connecticut 06030-6403, USA. graveley@neuron.uchc.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21179090" target="_blank"〉PubMed〈/a〉
Keywords:
Alternative Splicing/genetics
;
Animals
;
Base Sequence
;
Drosophila Proteins/genetics
;
Drosophila melanogaster/embryology/*genetics/*growth & development
;
Exons/genetics
;
Female
;
*Gene Expression Profiling
;
Gene Expression Regulation, Developmental/*genetics
;
Genes, Insect/genetics
;
Genome, Insect/genetics
;
Male
;
MicroRNAs/genetics
;
Oligonucleotide Array Sequence Analysis
;
Protein Isoforms/genetics
;
RNA Editing/genetics
;
RNA, Messenger/analysis/genetics
;
RNA, Small Untranslated/analysis/genetics
;
Sequence Analysis
;
Sex Characteristics
;
Transcription, Genetic/*genetics
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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