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  • 1
    Electronic Resource
    Electronic Resource
    A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 289-300 
    Details
    ISSN: 1432-2242
    Keywords: Morphological markers ; Isozymes ; Non-inbred species ; Combined map ; JoinMap
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic map of potato (Solanum tuberosum L.) integrating molecular markers with morphological and isozyme markers was constructed using a backcross population of 67 diploid potato plants. A general method for map construction is described that differs from previous methods employed in potato and other outbreeding plants. First, separate maps for the female and male parents were constructed. The female map contained 132 markers, whereas the male map contained 138 markers. Second, on the basis of the markers in common the two integrated parental maps were combined into one with the computer programme JoinMap. This combined map consisted of 175 molecular markers, 10 morphological markers and 8 isozyme markers. Ninety-two of the molecular markers were derived from DNA sequences flanking either T-DNA inserts in potato or reintegrated maize transposable elements originating from these T-DNA constructs. Clusters of distorted segregation were found on chromosomes 1,2,8 and 11 for the male parent and chromosome 5 for both parents. The total length of the combined map is 1120 cM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220891
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitatively-inherited resistance toGlobodera pallida is dominated by one major locus inSolanum spegazzinii (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 764-769 
    Details
    ISSN: 1432-2242
    Keywords: Potato cyst-nematode ; Quantitative trait loci RFLP ; Distorted segregation ; Solanum spegazzinii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A high level of resistance toGlobodera pallida pathotypes Pa2 and Pa3 exists inSolanum spegazzinii, a wild relative of potato (S. tuberosum ssp.tuberosum). Here we report the mapping of loci involved in quantitatively-inherited nematode resistance with the use of RFLPs. One major locus,Gpa, was mapped on chromosome 5 and two minor loci on chromosomes 4 and 7 ofS. spegazzinii. Additionally, the contribution of the susceptible parent to nematode resistance was determined. TheGpa locus was solely responsible for the high resistance level found in the segregating population. However, the RFLP marker closely linked to this resistance locus showed a distorted segregation, with a shortage of plants having the resistance linked allele. Our results indicate that a prediction of the genetic constitution of a quantitative trait based solely on phenotypic observations can lead to erroneous conclusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253983
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    Paper (German National Licenses)
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