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  • Chromatographie, Gas  (2)
  • Genetic diversity  (2)
  • Life Sciences (General)  (2)
  • Microsatellite  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 584-592 
    Details
    ISSN: 1432-2242
    Keywords: Key words Microsatellite markers ; Wheat ; Genetic diversity ; Genotype identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of 20 wheat microsatellite markers was used with 55 elite wheat genotypes to examine their utility (1) in detecting DNA polymorphism, (2)in the identifying genotypes and (3) in estimating genetic diversity among wheat genotypes. The 55 elite genotypes of wheat used in this study originated in 29 countries representing six continents. A total of 155 alleles were detected at 21 loci using the above microsatellite primer pairs (only 1 primer amplified 2 loci; all other primers amplified 1 locus each). Of the 20 primers amplifying 21 loci, 17 primers and their corresponding 18 loci were assigned to 13 different chromosomes (6 chromosomes of the A genome, 5 chromosomes of the B genome and 2 chromosomes of the D genome). The number of alleles per locus ranged from 1 to 13, with an average of 7.4 alleles per locus. The values of average polymorphic information content (PIC) and the marker index (MI) for these markers were estimated to be 0.71 and 0.70, respectively. The (GT)n microsatellites were found to be the most polymorphic. The genetic similarity (GS) coefficient for all possible 1485 pairs of genotypes ranged from 0.05 to 0.88 with an average of 0.23. The dendrogram, prepared on the basis of similarity matrix using the UPGMA algorithm, delineated the above genotypes into two major clusters (I and II), each with two subclusters (Ia, Ib and IIa, IIb). One of these subclusters (Ib) consisted of a solitary genotype (E3111) from Portugal, so that it was unique and diverse with respect to all other genotypes belonging to cluster I and placed in subcluster Ia. Using a set of only 12 primer pairs, we were able to distinguish a maximum of 48 of the above 55 wheat genotypes. The results demonstrate the utility of microsatellite markers for detecting polymorphism leading to genotype identification and for estimating genetic diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050077
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 341-345 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bread wheat ; Grain protein content ; Microsatellite ; STMS ; QTL analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study was undertaken with a view to tag gene(s) controlling grain protein content (GPC) using molecular markers in bread wheat. For this purpose, the genotype PH132 with high protein content (13.5%) was crossed with genotype WL711 with significantly lower protein content (9.7%), and 100 RILs were derived. These RILs showed normal distribution for protein content. The parental genotypes were analysed with 232 STMS primer pairs for detection of polymorphism. Of these, 167 primer pairs gave scorable amplification products, and 57 detected polymorphism between the parents. Using each of these 57 primer pairs, we carried out bulked segregant analysis on RILs representing the two extremes of the distribution. One primer pair for the locus wmc41 showed association with protein content. This was further confirmed through selective genotyping. The co-segregation data on the molecular marker (wmc41) and protein content on 100 RILs was analysed by means of a single-marker linear regression approach. Significant regression suggested linkage between wmc41 and a QTL (designated as QGpc.ccsu-2D.) for protein content. The results showed that this marker-linked QTL accounted for 18.73% of the variation for protein content between the parents. The marker has been located on chromosome arm 2DL using nulli-tetrasomic lines and two ditelocentric stocks for chromosome 2D.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051242
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  • 3
    Electronic Resource
    Electronic Resource
    Selection of diverse genotypes for heterosis in yield and response in toria (Brassica campestris L.) (1985)
    Springer
    Theoretical and applied genetics 69 (1985), S. 515-517 
    Details
    ISSN: 1432-2242
    Keywords: Toria ; Genetic diversity ; D2-correlation coefficient ; Heterosis ; Basic yield potential ; Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty-one genotypes of toria (Brassica campestris L.) were grown in twelve environments and subsequently analysed in order to select potential parents which expressed diversity for both 12 different characters (estimated by Mahalanobis' D2 technique) and response to the environments (estimated on the basis of negative correlation between deviations in seed yield of a pair of genotypes from their respective environmental means). Coefficients of determination (r2) were also used to measure the reliability of correlation, which is the basis of diversity of response. Stability parameters (b and S2d) and mean seed yield were also considered in selecting potential parents. On the basis of these criteria, three pairs of genotypes (ITSA and TCSU-1, TCSU-7 and TH-8 and Ludhiana Composite-1 and TH-4) are recommended to be used as parents for hybridization programmes so that heterosis both in seed yield and response may be exploited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251095
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of a microsatellite on chromosomes 6B and a STS on 7D of bread wheat showing an association with preharvest sprouting tolerance (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 336-340 
    Details
    ISSN: 1432-2242
    Keywords: Key words Preharvest sprouting ; Microsatellite ; STMS ; STS ; Linkage ; Bread wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In bread wheat, the transfer of tolerance to preharvest sprouting (PHS) that is associated with genotypes having red kernel colour to genotypes with amber kernels is difficult using conventional methods of plant breeding. The study here was undertaken to identify DNA markers linked with tolerance to PHS as these would allow indirect marker-assisted selection of PHS-tolerant genotypes with amber kernels. For this purpose, a set of 100 recombinant inbred lines (RILs) was developed using a cross between a PHS-tolerant genotype, SPR8198, with red kernels and a PHS-susceptible cultivar, ‘HD2329’, with white kernels. The two parents were analysed with 232 STMS (sequence-tagged microsatellite site) and 138 STS (sequence-tagged site) primer pairs. A total of 300 (167 STMSs and 133 STSs) primer pairs proved functional by giving scorable PCR products. Of these, 57 (34%) STMS and 30 (23%) STS primer pairs detected reproducible polymorphism between the parent genotypes. Using these primer pairs, we carried out bulked segregant analysis on two bulked DNAs, one obtained by pooling DNA from 5 PHS-tolerant RILs and the other similarly derived by pooling DNA from 5 PHS-susceptible RILs. Two molecular markers, 1 STMS primer pair for the locus wmc104 anda STS primer pair for the locus MST101, showed apparent linkage with tolerance to PHS. This was confirmed following selective genotyping of individual RILs included in the bulks. Chi-square contingency tests for independence were conducted on the cosegregation data collected on 100 RILs involving each of the two molecular markers (wmc104 and MST101) and PHS. The tests revealed a strong association between each of the markers and tolerance to PHS. Using nullisomic-tetrasomic lines, we were able to assign wmc104 and MST101 to chromosomes 6B and 7D, respectively. The results also indicated that the tolerance to PHS in SPR8198 is perhaps governed by two genes (linked with two molecular markers) exhibiting complementary interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051241
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  • 5
    Electronic Resource
    Electronic Resource
    Rapid quantitative gas-chromatographic analysis of 1,3,5-trioxane (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 410-411 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von 1,3,5-Trioxan ; Chromatographie, Gas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00468538
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  • 6
    Electronic Resource
    Electronic Resource
    Determination of 3-sulfolene in sulfolane by reaction gas chromatography (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 286 (1977), S. 219-221 
    Details
    ISSN: 1618-2650
    Keywords: Best. von 3-Sulfolen in Sulfolan ; Chromatographie, Gas ; Best. als 1,3-Butadien
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A simple gas-liquid chromatographic method has been developed for determining 3-sulfolene in sulfolane. The method involves thermal decomposition of sulfolene and determination of the 1.3-butadiene evolved, by GLC. Influence of the operating parameters on quantitative decomposition of sulfolene has been studied. The accuracy of the method as studied in the concentration range from 0.05 to 4.0 % (w/v) of 3-sulfolene in sulfolane is found to be within ±3.0%. It is rapid and sensitive down to 10 ppm and has potentialities for on-line applications also.
    Notes: Zusammenfassung Eine einfache gas-chromatographische Methode wurde entwickelt, die auf der thermischen Zersetzung der Probe und der Bestimmung des dabei aus 3-Sulfolen gebildeten 1,3-Butadiens beruht. Der Einfluß der einzelnen Parameter wurde untersucht. Innerhalb eines Konzentrationsbereichs von 0,05–4,0% 3-Sulfolen ergaben sich Fehler von ±3%. Das Verfahren ermöglicht bis herab zu 10 ppm eine schnelle und empfindliche Bestimmung und eignet sich auch zum on-line Betrieb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01155591
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  • 7
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    Validation of real-time three-dimensional echocardiography for quantifying left ventricular volumes in the presence of a left ventricular aneurysm: in vitro and in vivo studies (2000)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: OBJECTIVES: To validate the accuracy of real-time three-dimensional echocardiography (RT3DE) for quantifying aneurysmal left ventricular (LV) volumes. BACKGROUND: Conventional two-dimensional echocardiography (2DE) has limitations when applied for quantification of LV volumes in patients with LV aneurysms. METHODS: Seven aneurysmal balloons, 15 sheep (5 with chronic LV aneurysms and 10 without LV aneurysms) during 60 different hemodynamic conditions and 29 patients (13 with chronic LV aneurysms and 16 with normal LV) underwent RT3DE and 2DE. Electromagnetic flow meters and magnetic resonance imaging (MRI) served as reference standards in the animals and in the patients, respectively. Rotated apical six-plane method with multiplanar Simpson's rule and apical biplane Simpson's rule were used to determine LV volumes by RT3DE and 2DE, respectively. RESULTS: Both RT3DE and 2DE correlated well with actual volumes for aneurysmal balloons. However, a significantly smaller mean difference (MD) was found between RT3DE and actual volumes (-7 ml for RT3DE vs. 22 ml for 2DE, p = 0.0002). Excellent correlation and agreement between RT3DE and electromagnetic flow meters for LV stroke volumes for animals with aneurysms were observed, while 2DE showed lesser correlation and agreement (r = 0.97, MD = -1.0 ml vs. r = 0.76, MD = 4.4 ml). In patients with LV aneurysms, better correlation and agreement between RT3DE and MRI for LV volumes were obtained (r = 0.99, MD = -28 ml) than between 2DE and MRI (r = 0.91, MD = -49 ml). CONCLUSIONS: For geometrically asymmetric LVs associated with ventricular aneurysms, RT3DE can accurately quantify LV volumes.
    Keywords: Life Sciences (General)
    Type: Journal of the American College of Cardiology (ISSN 0735-1097); 36; 3; 900-7
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    NASA TECHNICAL REPORTS
  • 8
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    Manufacture of porous biodegradable polymer conduits by an extrusion process for guided tissue regeneration (1998)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: We have fabricated porous, biodegradable tubular conduits for guided tissue regeneration using a combined solvent casting and extrusion technique. The biodegradable polymers used in this study were poly(DL-lactic-co-glycolic acid) (PLGA) and poly(L-lactic acid) (PLLA). A polymer/salt composite was first prepared by a solvent casting process. After drying, the composite was extruded to form a tubular construct. The salt particles in the construct were then leached out leaving a conduit with an open-pore structure. PLGA was studied as a model polymer to analyze the effects of salt weight fraction, salt particle size, and processing temperature on porosity and pore size of the extruded conduits. The porosity and pore size were found to increase with increasing salt weight fraction. Increasing the salt particle size increased the pore diameter but did not affect the porosity. High extrusion temperatures decreased the pore diameter without altering the porosity. Greater decrease in molecular weight was observed for conduits manufactured at higher temperatures. The mechanical properties of both PLGA and PLLA conduits were tested after degradation in vitro for up to 8 weeks. The modulus and failure strength of PLLA conduits were approximately 10 times higher than those of PLGA conduits. Failure strain was similar for both conduits. After degradation for 8 weeks, the molecular weights of the PLGA and PLLA conduits decreased to 38% and 43% of the initial values, respectively. However, both conduits maintained their shape and did not collapse. The PLGA also remained amorphous throughout the time course, while the crystallinity of PLLA increased from 5.2% to 11.5%. The potential of seeding the conduits with cells for transplantation or with biodegradable polymer microparticles for drug delivery was also tested with dyed microspheres. These porous tubular structures hold great promise for the regeneration of tissues which require tubular scaffolds such as peripheral nerve, long bone, intestine, or blood vessel.
    Keywords: Life Sciences (General)
    Type: Biomaterials (ISSN 0142-9612); 19; 21; 1945-55
    Format: text
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    NASA TECHNICAL REPORTS
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