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  • Chromatin structure Linker histones H1t-deficient mice Spermatogenesis  (1)
  • histone H2A  (1)
  • histone pseudogene  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Spermatogenesis proceeds normally in mice without linker histone H1t (2000)
    Springer
    Histochemistry and cell biology 113 (2000), S. 433-442 
    Details
    ISSN: 1432-119X
    Keywords: Chromatin structure Linker histones H1t-deficient mice Spermatogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The histone gene H1t is expressed exclusively in pachytene spermatocytes of the testis. In this report we have eliminated the single copy H1t gene by homologous recombination from the mouse genome to analyse the function of the H1t protein during spermatogenesis. Mice homozygous for the mutated H1t gene locus developed normally and showed no anatomic abnormalities until the adult stage. In addition, H1t-deficient mice were fertile and reproduced as wild-type mice. The process of spermatogenesis and the testicular morphology remained unchanged in the absence of H1t. RNase protection analysis demonstrated that H1.1, H1.2 and H1.4 histone gene expression is enhanced during spermatogenesis in H1t-deficient mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180000146
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  • 2
    Electronic Resource
    Electronic Resource
    Association of a human H1 histone gene with an H2A pseudogene and genes encoding H2B.1 and H3.1 histones (1993)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 52 (1993), S. 375-383 
    Details
    ISSN: 0730-2312
    Keywords: histone H1 ; histone H2A ; histone H2B ; histone H3 ; histone genes ; histone pseudogene ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: A cluster of human histone genes was found on three overlapping clones isolated from cosmid and bacteriophage libraries. These three overlapping segments of the human genome comprise genes coding for H3.1, an H2A pseudogene, and an H2B.1 gene downstream of the previously characterized H1.2 gene. The cosmid clone covers 30 kb upstream of the H1.2 gene and overlaps with two phage clones covering the core histone genes and the pseudogene. The same arrangement of an H3 gene, an H2A pseudogene and an H2B gene downstream of an H1 gene has been described within a mouse histone gene cluster [Yang et al.: J Biol Chem 262:17118-17125, 1987; Gruber et al.: Gene 95:303-304, 1990].
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240520402
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