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Spermatogenesis proceeds normally in mice without linker histone H1t

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Histochemistry and Cell Biology Aims and scope Submit manuscript

Abstract.

The histone gene H1t is expressed exclusively in pachytene spermatocytes of the testis. In this report we have eliminated the single copy H1t gene by homologous recombination from the mouse genome to analyse the function of the H1t protein during spermatogenesis. Mice homozygous for the mutated H1t gene locus developed normally and showed no anatomic abnormalities until the adult stage. In addition, H1t-deficient mice were fertile and reproduced as wild-type mice. The process of spermatogenesis and the testicular morphology remained unchanged in the absence of H1t. RNase protection analysis demonstrated that H1.1, H1.2 and H1.4 histone gene expression is enhanced during spermatogenesis in H1t-deficient mice.

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Authors and Affiliations

  1. Zentrum Biochemie und Molekulare Zellbiologie, Abt. Molekularbiologie, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany, , , , ,

    Birgit Drabent, Christa Bode & Detlef Doenecke

  2. Zentrum Biochemie und Molekulare Zellbiologie, Abt. Biochemie II, Universität Göttingen, Heinrich-Düker-Weg 12, 37073 Göttingen, Germany, , , , ,

    Paul Saftig

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  1. Birgit Drabent
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  2. Paul Saftig
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  3. Christa Bode
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  4. Detlef Doenecke
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Drabent, B., Saftig, P., Bode, C. et al. Spermatogenesis proceeds normally in mice without linker histone H1t. Histochem Cell Biol 113, 433–442 (2000). https://doi.org/10.1007/s004180000146

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  • DOI: https://doi.org/10.1007/s004180000146

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