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  • Chlamydomonas  (2)
  • Flagellar regeneration  (1)
  • Nitrate transport  (1)
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  • 1
    ISSN: 1432-0983
    Keywords: Chlamydomonas reinhardtii ; Flagellar regeneration ; ts Mutants ; uni Linkage group
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated a mutant of Chlamydomonas reinhardtii that is both temperature sensitive for viability and temperature sensitive for flagellar regeneration. The mutation (designated tnr1, for temperature-sensitive nonregenerator) has been genetically mapped to a position near uni1 on the uni linkage group (ULG), an unusual genetically circular linkage group consisting primarily of mutations affecting flagellar assembly or function. tnr1 is the first essential gene identified on this linkage group, and is one of the few essential genes affecting flagellar function identified to date. We also find that tnr1 cells are not defective for induction of new tubulin transcripts or protein synthesis during flagellar regeneration at the nonpermissive temperature, and that at least a portion of the unassembled pool of flagellar proteins in mutant cells is assembly-competent at the nonpermissive temperature.
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  • 2
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Restriction fragment length polymorphisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new field isolate of the unicellular green algaChlamydomonas reinhardtii with useful properties for restriction fragment length polymorphism mapping is described in this report. The isolate, S1-D2 (mating type-), was the only strain found among 24Chlamydomonas isolates taken from many locations which was interfertile with laboratory strains ofC. reinhardtii. It mates at high efficiency, giving tetrads with excellent viability. Using cloned probes for both nuclear and chloroplast genes, we have found numerous restriction fragment length polymorphisms between Sl-D2 and laboratory strains ofC. reinhardtii.
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  • 3
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Drug resistance ; Pleiotropy ; Dominance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three independent pleiotropic drug-resistance (pdr) mutants were isolated by selecting for resistance to the anti-microtubule herbicides amiprophos-methyl (APM) and oryzalin (ORY). These three mutants and a previously isolated mutant, ani1 (anisomycin resistance), were semi-dominant in heterozygous diploids, and they displayed varying degrees of resistance to structurally and functionally unrelated inhibitors such as cycloheximide, cryptopleurine, emetine, atrazine, and nonidet P-40. Linkage analysis and genetic mapping suggested that three of the four mutants, including ani1, define a single locus, here named pdr1. The fourth mutant defined a new locus, pdr2, which is located on the left arm of linkage group VI. One pdr1 mutant exhibited unusual genetic interactions, including enhanced ts-lethality and synergistic increases in drug resistance, when combined with pdr2-1 and with herbicide-resistant alleles of three other genes.
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  • 4
    ISSN: 1617-4623
    Keywords: Gene cluster ; Nitrate transport ; Regulatory mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three overlapping clones covering a Chlarnydomonas reinhardtii genomic region of about 32 kb appear to contain five genes potentially involved in nitrate assimilation in addition to the nitrate reductase structural locus nit-1. These new loci produced transcripts of 2.8, 2.2, 1.8 and 1.7 kb in nitrate-induced wild-type cells that, like the 3.4 kb transcript of nit-1, were undetectable in cells grown in ammonium. In addition, in a mutant defective at the regulatory locus, nit-2 for nitrate assimilation, which does not express the nit-1 gene transcript, accumulation of the four other transcripts was also blocked. They have been named nar (nitrate assimilation related) genes. The nar-1 and nar-2 loci are transcribed in the same orientation as nit-1. The nar-3 and nar-4 loci are transcribed divergently from nit-1. DNA and RNA sequences from both nar-3 and nar-4 cross-hybridized with each other indicating that they share similar sequences. Four nitrate assimilation-deficient mutants (C2, D2, F6 and G1) were characterized. These mutants lack nar transcripts and have major deletions and/or rearrangements in the nar gene cluster. In contrast to other nitrate reductase-deficient mutants and to wild type, deletion mutants and the regulatory mutant nit-2 were incapable of accumulating intracellular nitrate. Two of the mutants in which expression of all of the nar loci did not occur, C2 and D2, grew in nitrite medium and showed wild-type levels of both nitrite uptake and nitrite reductase activities. Thus the nar loci cannot be required for nitrite assimilation. Mutants F6 and G1 were unable to grow in either nitrite- or nitrate-containing medium, and lacked nitrate reductase, nitrite reductase, nitrate uptake and nitrite uptake activities. The inability to assimilate nitrite co-segregated with nit-1 in crosses between these mutants and wild type. These results indicate that a complex gene cluster responsible for the assimilation of nitrate has been identified in C. reinhardtii, and that, in addition, at least one locus necessary for nitrite assimilation is genetically linked to this cluster.
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