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  • Chemistry  (16)
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  • 1
    Electronic Resource
    Electronic Resource
    Packed column supercritical fluid chromatography/mass spectrometry using a thermospray source in the filament-on mode (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 105-109 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Combined packed column supercritical fluid chromatography/mass spectrometry has been effected using a thermospray ion source in the filament-on mode. If organic modifiers are used with carbon dioxide as the supercritical mobile phase, chemical ionization mass spectra are obtained. With carbon dioxide electron impact type mass spectra are obtained. The potential of the system is illustrated with studies of mixtures of carbamate and organochlorine pesticides and a crude ergot fermentation extract.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200150208
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  • 2
    Electronic Resource
    Electronic Resource
    The cellulose-binding domain (CBDCex) of an exoglucanase from Cellulomonas fimi: Production in Escherichia coli and characterization of the polypeptide (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 42 (1993), S. 401-409 
    Details
    ISSN: 0006-3592
    Keywords: cellulose-binding domain ; cellulase ; cellulose ; adsorption ; affinity chromatography ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The gene fragment encoding the cellulose-binding domain (CBD) of an exoglucanase (Cex) from Cellulomonas fimi was subcloned and expressed in Escherichia coli. Transcription from the lac promoter coupled with translation from a consensus prokaryotic ribosome binding site led to the production of large quantities of CBDCex (up to 25% total soluble cell protein). The polypeptide leaked into the culture supernatant (up to 50 mg · L-1), facilitating one-step purification by affinity chromatography on cellulose. The 11-kDa polypeptide reacted with Cex antiserum. Absence of free thiols indicated that the two Cys residues of CBDCex form a disulfide bridge. It had the same N-terminal amino acid sequence as CBDCex prepared from Cex by proteolysis, plus two additional N-terminal amino acid residues (Ala and Ser) encoded by the Nhel site introduced during plasmid construction. CBDCex bound to a variety of β-1, 4-glycans with different affinities and saturation levels. Adsorption to bacterial microcrystalline cellulose was dependent on the temperature, but not on the pH. © 1993 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260420402
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  • 3
    Electronic Resource
    Electronic Resource
    Purification and processing of cellulose-binding domain-alkaline phosphatase fusion proteins (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 44 (1994), S. 1295-1305 
    Details
    ISSN: 0006-3592
    Keywords: Escherichia coli ; fusion proteins ; Cellulomonas fimi ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fusion of the leader peptide and the cellulose-binding domain (CBD) of endoglucanase A (CenA) from Cellulomonas fimi, with of without linker sequences, to the N-terminus of alkaline phosphatase (PhoA) from Escherichia coli leads to the accumulation of significant amounts of the CBD-PhoA fusion proteins in the supernatants of E. coli cultures. The fusion proteins can be purified from the supernatants by affinity chromatography on cellulose. The fusion protein can be desorbed from the cellulose with water or guanidine-HCl. If the sequence IEGR in present between the CBD and PhoA, the CBD can be cleaved from the PhoA with factor Xa. The efficiency of hydrolysis by factor Xa is strongly in fluenced by the amino acids on either side of the IEGR sequence. The CBD released by factor Xa is removed by adsorption to cellulose. A nonspecific proteases from C. fimi, which hydrolyzes native CenA between the CBD and the catalytic domain, may be useful for removing the CBD from some fusion proteins. © 1994 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260441105
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  • 4
    Electronic Resource
    Electronic Resource
    Adhesion of mammalian cells to a recombinant attachment factor, CBD/RGD, analyzed by image analysis (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 46 (1995), S. 185-193 
    Details
    ISSN: 0006-3592
    Keywords: image analysis ; cell attachment ; mammalian cells ; cellulase ; Cellulomonas fimi ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A luminance thresholding procedure was developed to quantify cell attachment of a variety of cell lines to CBD/RGD, a hybrid attachment factor comprising a cellulose binding domain and the fibronectin-like RGD attachment peptide. The technique used local thresholding, median filtering, and opening to separate and count cells on each image. Cell lines exhibited three different patterns of attachment to CBD/RGD, depending on whether it was immobilized on polystyrene or cellulose acetate. Vero, COS, HFF, 3T3, 293, and U373 cells attached well to CBD/RGD immobilized on polystyrene or cellulose acetate. CHO, MRC-5, and HEp-2 cells attached to CBD/RGD immobilized on polystyrene, but not to CBD/RGD immobilized on cellulose acetate. BHK and L cells failed to attach to CBD/RGD immobilized on either polystyrene or cellulose acetate. The attachment of many cell lines to CBD/RGD was comparable with attachment of these cells to fibronectin. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260460302
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  • 5
    Electronic Resource
    Electronic Resource
    Succinimide-mediated pathway for peptide bond cleavage: Kinetic study on an Asn-Sar containing peptide (1996)
    New York : Wiley-Blackwell
    Biopolymers 40 (1996), S. 543-551 
    Details
    ISSN: 0006-3525
    Keywords: peptide bond cleavage ; asparagine deamidation ; succinimide ring formation ; kinetics and mechanism ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The cleavage reaction of the peptide bond next to the Asn residue has been studied in the pH range 7.4-13.8 at 37°C and μ = 1M. This reaction yields an N-terminal peptide fragment having at its C-terminus a succinimide ring, which rapidly hydrolyses to both asparagine and iso-asparagine residues.For both the two consecutive reactions, peptide bond cleavage and the succinimide hydrolysis, the general trend is an increase of the reaction rate with the pH. However, for the hydrolysis reaction there is a small decrease in the pH range 10-11 caused by the deprotonation of the succinimide nitrogen atom. Kinetic evidence indicates that the cleavage reaction is a multistep process with a change in the rate-determining step at pH 8.5-9.0. The mechanism involves preequilibrium deprotonation of the NH2 amide group of the Asn side chain, followed by nucleophilic attack of the nitrogen atom on the carbonyl carbon atom of the same asparagine residue, giving a cyclic intermediate. Then, general acid-catalyzed departure of the leaving group gives the final reaction product. At pH 〈 8.5, the formation of the cyclic intermediate is rate determining, whereas, at higher pH, it is the departure of the leaving group. © 1997 John Wiley & Sons, Inc. Biopoly 40: 543-551, 1996
    Additional Material: 6 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    Production and properties of a bifunctional fusion protein that mediates attachment of vero cells to cellulosic matrices (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 47 (1995), S. 147-154 
    Details
    ISSN: 0006-3592
    Keywords: arg-gly-asp ; cellulose ; protein production ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The sequence Arg-Gly-Asp (RGD) in extracellular matrix proteins such as fibronectin, collagen, and laminin mediates cell attachment by interacting with proteins of the integrin family of cell surface receptors. A gene fusion encoding the RGD-containing peptide, fused to the C-terminus of a cellulose-binding domain (CBD/RGD), was expressed in Escherichia coli. Cultures produced up to 50 mg of CBD/RGD per liter, most of which was extracellular. It was purified from the culture supernatant by affinity chromatography on cellulose. CBD/RGD promoted the attachment of green monkey Vero cells to polystyrene and cellulose acetate. Attachment was inhibited by small synthetic peptides containing the RGD sequence. CBD/RGD was as effective as collagen in promoting the attachment of Vero cells to Cellsnow™ microcarriers. © 1995 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260470205
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  • 7
    Electronic Resource
    Electronic Resource
    Photolyse der Cyclopentadienyl(hydrido)komplexe C5H5M(CO)3H (M = Cr, Mo, W) in Matrix und in Lösung; Charakterisierung von [C5H5W(CO)2H]2 und [C5Me5W(CO)2H]2Diese Arbeit wurde von der Deutschen Forschungsgemeinschaft und dem Fonds der Chemischen Industrie unterstützt. (1983)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 95 (1983), S. 569-570 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19830950722
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  • 8
    Electronic Resource
    Electronic Resource
    Desulfurierung von Benzo[b]thiophen durch S/Ru-Austausch: Bildung und Struktur von [Ru3(CO)8(C8H6)]Diese Arbeit wurde vom Science and Engineering Research Council (SERC), vom Consejo Nacional de Investigaciónes Cientificas y Tecnológieas (CONICIT, Venezuela) und vom University of London Central Research Fund gefördert. (1994)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 106 (1994), S. 1459-1461 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19941061327
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  • 9
    Electronic Resource
    Electronic Resource
    1H and 13C chemical shift assignments of para-substituted aryl 2-acetamido-2-deoxy-β-D-glucopyranosides (1991)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 29 (1991), S. 852-858 
    Details
    ISSN: 0749-1581
    Keywords: 13C NMR ; 1H NMR ; 3J(CH) ; Glucopyranosides ; Aryl 2-acetamido-2-deoxy-β-D-glucopyranosides ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 1H and 13C NMR spectra of twenty aryl 2-acetamido-2-deoxy-β-D-glucopyranosides and eighteen aryl 3,4,6-tri-O-acetyl-2-acetamido-2-deoxy-β-D-glucopyranosides have been obtained and assigned. The three-bond proton coupling constants of these compounds were also obtained.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260290817
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  • 10
    Electronic Resource
    Electronic Resource
    Carbon-13 NMR relaxation study of the overall and internal motions in compounds containing n-octyl chains (1991)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 29 (1991), S. 273-281 
    Details
    ISSN: 0749-1581
    Keywords: Molecular motion ; Carbon-13 relaxation ; Octyl chains ; Polydecene ; Model lubricants ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 13C nuclear relaxation behaviour of n-alkyl chains in a variety of compounds can be described in terms of two relaxation times, one for tumbling (τc) and one for each carbon nucleus for the internal motions (τc). Relaxation data for the quaternary carbon nuclei of n-octylbenzene and n-octyl cyanide have been used to determine τc) and their activation energies [Ea(τc)]. These values have enabled τe and their associated activation energies [Ea(τe)] to be calculated for each carbon in the alkyl chains of the two compounds. In these compounds the values for C-6 appear to be invariant with τe(298) = 15 ± 2 ps and Ea(τc) = 15 ± 1 kJ mol-1. Assuming that these are universal values for all n-octyl chains, we have used them to calculate τe and Ea(τe) for other simple n-octyl compounds, thus allowing τc and E.a(τc) to be determined. It was found that Ea(τc) values correlate well with those for Ea(n/T) obtained from viscosity measurements. Di-n-octyl ether shows motional properties similar to those of the straightchain compounds.Simultaneous fitting of relaxation rate and NOE enhancement data measured over a range of radiofrequencies and temperatures permits a wealth of motional properties to be discerned when there is a suitable frequency dispersion. Both tri-n-octylamine and polydecene tumble sufficiently slowly to allow both relaxation rate and NOE data to be used to evaluate τc and τe and their activation energies without making any assumptions about C-6. The values of τc(298) and Ea(τc) obtained from C-6 for tri-n-octylamine are 314 ± 12 ps and 18.5 ± 0.4 kJ mol-1, respectively, and the corresponding values for polydecene are 576 ± 41 ps and 16.1 ± 1.3 kJ mol-1, respectively.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260290314
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