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  • Cell suspension culture  (2)
  • Costa Rica  (1)
  • 1
    ISSN: 1432-203X
    Keywords: Key wordsSolanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines: ccvx (cotyledon derived) and ccvz (hypocotyl derived) of Solanum chrysotrichum were cultivated in 10-l airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l–1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with the ccvx cell line (14.6 g l–1) than with ccvz (7.7 g l–1), resulting in 23 and 12 mg g–1 of SC-1 after 17 days in culture for ccvx and ccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l–1 day–1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l–1 day–1, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite of S. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Solanum chrysotrichum ; Cell suspension culture ; Spirostanol saponins ; Antimycotic ; Large-scale culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of two different cell lines:ccvx (cotyledon derived) andccvz (hypocotyl derived) ofSolanum chrysotrichum were cultivated in 10-1 airlift bioreactors for the production of the human antimycotic compound SC-1. When using 3 g l-1 dry weight inoculum in a batch culture, higher levels of biomass were achieved with theccvx cell line (14.6 g l-1) than withccvz (7.7 g l-1), resulting in 23 and 12 mg g-1 of SC-1 after 17 days in culture forccvx andccvz, respectively. The maximum productivity of SC-1 in bioreactors was 0.025 g l-1 day-1 after 9 days in culture. When using a draw-fill mode, the productivity increased by 60% to a value of 0.041 g l-1 day, 4 days after 50% of the cell suspension was removed and replaced with fresh medium. This latter bioreactor system is a feasible alternative for the production of the antimycotic metabolite ofS. chrysotrichum on a large scale.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of seismology 3 (1999), S. 177-190 
    ISSN: 1573-157X
    Keywords: travel time tomography ; Costa Rica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract 45287 P-wave and 26813 S-wave arrival times from the data base of the Costa Rica network have been tomographically inverted to image the structure beneath Costa Rica. A regularized recursive least squares inverse method was used to produce the high resolution and minimum variance model parameter estimates. The first arrival times are calculated using a finite difference technique, which allows for flexible parameterization of the velocity model and easy inclusion of topography and source-receiver geometry. The P wave velocity structure and hypocenters are determined simultaneously, while the S wave velocity structure is determined using the relocated seismicity and an initial model derived from the P wave model assuming an average P to S wave velocity ratio of 1.78. The most prominent features in the inverted model are a low velocity structure under the volcanic chain in the center of the country, which is related to the hot material connected with the active volcanoes; and a high velocity zone in the mantle, which is related to the Cocos plate subducted under Costa Rica.
    Type of Medium: Electronic Resource
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