Publication Date:
2000-08-11
Description:
Previous work has identified two families of proteins that transport classical neurotransmitters into synaptic vesicles, but the protein responsible for vesicular transport of the principal excitatory transmitter glutamate has remained unknown. We demonstrate that a protein that is unrelated to any known neurotransmitter transporters and that was previously suggested to mediate the Na(+)-dependent uptake of inorganic phosphate across the plasma membrane transports glutamate into synaptic vesicles. In addition, we show that this vesicular glutamate transporter, VGLUT1, exhibits a conductance for chloride that is blocked by glutamate.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bellocchio, E E -- Reimer, R J -- Fremeau, R T Jr -- Edwards, R H -- New York, N.Y. -- Science. 2000 Aug 11;289(5481):957-60.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurology, University of California at San Francisco School of Medicine, 513 Parnassus Avenue, San Francisco, CA 94143-0435, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10938000" target="_blank"〉PubMed〈/a〉
Keywords:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology
;
Adenosine Triphosphate/metabolism
;
Animals
;
Biological Transport, Active/drug effects
;
Carrier Proteins/genetics/*metabolism
;
Cell Membrane/metabolism
;
Chlorides/metabolism
;
Glutamic Acid/*metabolism
;
Hydrogen-Ion Concentration
;
PC12 Cells
;
Phosphates/metabolism
;
Potassium Chloride/metabolism
;
Rats
;
Sodium-Phosphate Cotransporter Proteins
;
*Symporters
;
Synaptic Vesicles/*metabolism
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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