Publication Date:
2005-01-22
Description:
In contrast to most genomic DNA in mitotic cells, the promoter regions of some genes, such as the stress-inducible hsp70i gene that codes for a heat shock protein, remain uncompacted, a phenomenon called bookmarking. Here we show that hsp70i bookmarking is mediated by a transcription factor called HSF2, which binds this promoter in mitotic cells, recruits protein phosphatase 2A, and interacts with the CAP-G subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. Blocking HSF2-mediated bookmarking by HSF2 RNA interference decreases hsp70i induction and survival of stressed cells in the G1 phase, which demonstrates the biological importance of gene bookmarking.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xing, Hongyan -- Wilkerson, Donald C -- Mayhew, Christopher N -- Lubert, Eric J -- Skaggs, Hollie S -- Goodson, Michael L -- Hong, Yiling -- Park-Sarge, Ok-Kyong -- Sarge, Kevin D -- GM61053/GM/NIGMS NIH HHS/ -- GM64606/GM/NIGMS NIH HHS/ -- HD36879/HD/NICHD NIH HHS/ -- HD41609/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 2005 Jan 21;307(5708):421-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular and Cellular Biochemistry, Chandler Medical Center, University of Kentucky, Lexington, KY 40536, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15662014" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphatases/metabolism
;
Cell Line, Tumor
;
Chromatin Immunoprecipitation
;
DNA-Binding Proteins/metabolism
;
*Gene Expression Regulation
;
HSP70 Heat-Shock Proteins/*genetics
;
HeLa Cells
;
Heat-Shock Proteins/genetics/*metabolism
;
Hot Temperature
;
Humans
;
Immunoprecipitation
;
Interphase
;
*Mitosis
;
Multiprotein Complexes
;
Phosphoprotein Phosphatases/metabolism
;
Phosphorylation
;
*Promoter Regions, Genetic
;
Protein Binding
;
Protein Phosphatase 2
;
Protein Subunits/metabolism
;
RNA Interference
;
RNA, Small Interfering/pharmacology
;
Transcription Factors/genetics/*metabolism
;
Two-Hybrid System Techniques
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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