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  • 1
    Electronic Resource
    Electronic Resource
    Immunochemical studies of the cellulose synthase complex inAcetobacter xylinum (1996)
    Springer
    Cellulose 3 (1996), S. 63-75 
    Details
    ISSN: 1572-882X
    Keywords: antibodies ; cellulose synthase ; Acetobacter xylinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract An immunochemical method was used to analyse the 83 and 93 Kd polypeptides of cellulose synthase from Acetobacter xylinum.Polyclonal antibodies were raised against the LDS-PAGE-fractionated 83 and 93 Kd polypeptides isolated from A. xylinum.Using these antibodies, the 83 and 93 Kd polypeptides were localized in the different fractions during purification of cellulose synthase, and the ratio of these two polypeptides was determined to be 1∶1. A differential solubilization of the 83 and 93 Kd polypeptides from the cell strongly suggested that the mechanism by which these two polypeptides originate from a single acsAB gene product (Saxena et al.,1994) must be via a post-translational cleavage. The results of trypsin treatment of the membrane fraction used in the purification of cellulose synthase were analysed to determine the fate of these two polypeptides and their relationship to the enzyme activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02228791
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  • 2
    Electronic Resource
    Electronic Resource
    Characterizing the product crystals from a mixing tee process (1988)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 34 (1988), S. 2063-2068 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690341216
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  • 3
    Electronic Resource
    Electronic Resource
    Axial dispersion coefficient measurement in two-phase flow (1968)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 14 (1968), S. 657-658 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690140422
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  • 4
    Electronic Resource
    Electronic Resource
    Acetylcholine and cholinesterase in the insect central nervous systemThe authors wish to thank Prof. Kenneth D. Roeder of Tufts College for his invaluable technical advice and helpful criticism. (1941)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 18 (1941), S. 401-403 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030180312
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  • 5
    Electronic Resource
    Electronic Resource
    Pattern and process of wall formation in developing endosperm (1995)
    New York, NY : Wiley-Blackwell
    BioEssays 17 (1995), S. 803-812 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Endosperm is emerging as a system for investigating the genetic control of wall placement and deposition in plant development. Development of endosperm progresses in distinct stages from a wall-less syncytial stage to a cellular stage that is entirely typical of plant meristems where the division plane is predicted by a preprophase band of microtubules (PPB) and cytokinesis is completed by formation of a cell plate in association with a phragmoplast. Four developmentally different types of walls, each associated with a different microtubule system, are sequentially produced: (1) free growing walls deposited in the absence of mitosis and phragmoplasts; (2) walls guided by cytoplasmic phragmoplasts formed adventitiously in the absence of mitosis; (3) walls formed by interzonal phragmoplasts in a cell cycle that lacks PPBs; and (4) wall deposition driven by interzonal phragmoplasts in a cycle that includes PPBs. We are using methods of differential screening to isolate cDNA clones corresponding in temporal and spatial pattern to the types of wall development, and are studying mutants for clues to the genetic controls of wall development.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950170910
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  • 6
    Electronic Resource
    Electronic Resource
    Practical principles of die design - a simplified procedure, in table form, for rigid PVC (1981)
    Brookfield, Conn. : Wiley-Blackwell
    Journal of Vinyl and Additive Technology 3 (1981), S. 215-218 
    Details
    ISSN: 0193-7197
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An approach to simplifying die design for rigid PVC is proposed. Previous work, in this area, has been considered too complicated to be of use to the average die designer. It is hoped that the provided information, simplified equations and tabular format can make it easier to achieve good dies for this important processing technique.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/vnl.730030404
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of the physical structure of biological materials at biosensor interfaces by techniques of increasing magnification from microscopic to molecular scale (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 18 (1991), S. 212-222 
    Details
    ISSN: 0741-0581
    Keywords: Scanning tunneling microscopy ; Langmuir-Blodgett films ; Covalent deposition ; Lipids ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Chemical selectivity of biosensors is derived from biological materials interfaced to the surface of transducing devices. Molecular recognition events lead to macroscopic function suitable for analytical measurements. The structure-function relationships of biochemical species at interfaces must be established to characterize and optimize biosensor operation. The techniques of ellipsometry, fluorescence microscopy, electron microscopy, and scanning tunneling microscopy are used to investigate the structure of monolayers and multilayers of proteins and lipids at interfaces that are prepared by Langmuir-Blodgett techniques and by self-assembly from bulk solution. The relative merits and limitations of the measurement techniques in the determination of aspects of interfacial structure are considered.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060180303
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  • 8
    Electronic Resource
    Electronic Resource
    Practical Aspects of Lattice Imaging of Cellulose (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 6 (1987), S. 349-356 
    Details
    ISSN: 0741-0581
    Keywords: Lattice imaging ; Low dose technique ; Cellulose ; Crystallite size ; Digital image processing ; Formvar micronet ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The lattice imaging technique for cellulose, a typical electronbeam-sensitive material, was developed by using a conventional 120 kV electron microscope. Routine procedures for specimen preparation and high resolution, low dose electron microscopy are described in detail. A new, simple method was introduced for the preparation of a Formvar micronet to support the thin carbon film. The lattice imaging technique was successfully applied to algal celluloses as well as bacterial cellulose, which is composed of much smaller crystallites than the former. Digital image processing was found to be effective in enhancing the lattice images. The bacterial cellulose ribbon contained crystallites 10-25-nm wide, which is much greater than the basic unit of cellulose fibril extruded from the cell surface. This shows that unit fibrils can fasciate with each other, merging into a single crystallite.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060060405
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