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  • 1
    ISSN: 1617-4623
    Keywords: Key words Saccharomyces cerevisiae ; Calcium ; Copper ; Sphingolipid ; CSG1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saccharomyces cerevisiae cells require two genes, CSG1/SUR1 and CSG2, for growth in 50 mM Ca2+, but not 50 mM Sr2+. CSG2 was previously shown to be required for the mannosylation of inositol-phosphorylceramide (IPC) to form mannosylinositolphosphorylceramide (MIPC). Here we demonstrate that SUR1/CSG1 is both genetically and biochemically related to CSG2. Like CSG2, SUR1/CSG1 is required for IPC mannosylation. A 93–amino acid stretch of Csg1p shows 29% identity with the α-1, 6-mannosyltransferase encoded by OCH1. The SUR1/CSG1 gene is a dose-dependent suppressor of the Ca2+-sensitive phenotype of the csg2 mutant, but overexpression of CSG2 does not suppress the Ca2+ sensitivity of the csg1 mutant. The csg1 and csg2 mutants display normal growth in YPD, indicating that mannosylation of sphingolipids is not essential. Increased osmolarity of the growth medium increases the Ca2+ tolerance of csg1 and csg2 mutant cells, suggesting that altered cell wall synthesis causes Ca2+-induced death. Hydroxylation of IPC-C to form IPC-D requires CCC2, a gene encoding an intracellular Cu2+ transporter. Increased expression of CCC2 or increased Cu2+ concentration in the growth medium enhances the Ca2+ tolerance of csg1 mutants, suggesting that accumulation of IPC-C renders csg1 cells Ca2+ sensitive.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 400 (1999), S. 123-128 
    ISSN: 1573-5117
    Keywords: exotic species ; leaf litter ; C/N ratios ; decomposition ; stream ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Russian olive tree (Elaeagnus angustifolia L.) was brought to the western United States from Eurasia during the early to mid-1900s, and has since become a common member of many riparian communities in Idaho. We compared leaf chemistry and in-stream processing of Russian olive leaves (exotic) and various species of native leaves in one hardwater and one relatively softwater Idaho stream. Measurements using air-dried leaves showed that Russian olive contained the greatest concentration of nitrogen, approximately 1.6% of the dry mass, whereas the native species each contained less than 1.0% nitrogen. The C/N ratio of Russian olive was 〈30, whereas the natives each had C/N ratios greater than 40. Results from the hardwater stream indicated no difference in 30-day loss of AFDM between Russian olive and the native leaves (dogwood and aspen). In the relatively softwater stream, the Russian olive leaves were processed significantly slower than the native leaf species (cottonwood). The results indicate that a replacement of native riparian trees by exotics, such as Russian olive, may result in slower rates of leaf processing in Idaho streams but that the effect may vary among streams. When comparing the processing of native and exotic leaf litter, initial nitrogen concentrations and initial C/N ratios of the leaves did not appear to be accurate indicators of relative decay rates.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 20 (1999), S. 1051-1060 
    ISSN: 1572-9567
    Keywords: copper ; liquid metals ; liquid alloys ; Lorenz relation ; resistivity ; thermal conductivity ; thermal diffusivity ; tin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The thermal properties (heat capacity, thermal diffusivity, and electrical resistivity) of a Cu + 10 wt% Sn alloy in both solid and liquid phases have been reported. Using these values it was confirmed that the Lorenz relation is suitable for obtaining thermal conductivity from electrical resistivity in the liquid phase of this alloy. Also, the temperature differential (dλ/dT) obtained from such an approach was in excellent agreement with the thermal conductivity values calculated from thermal diffusivity.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 20 (1999), S. 677-690 
    ISSN: 1572-9567
    Keywords: liquid metals ; liquid alloys ; Lorenz relation ; resistivity ; thermal conductivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A four-probe dc technique for measuring liquid-metal electrical resistivities has been developed, and liquid-resistivity measurements on gallium, tin, lead, copper, a lead tin alloy, a copper tin alloy, and a zinc alloy have been presented. The Lorenz relation has been used to calculate thermal conductivities from these resistivity measurements. The thermal conductivities calculated from these resistivity measurements are (where data are available) in good agreement with recommended liquid-metal thermal conductivities.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 400 (1999), S. 123-128 
    ISSN: 1573-5117
    Keywords: exotic species ; leaf litter ; C/N ratios ; decomposition ; stream ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Russian olive tree (Elaeagnus angustifolia L.) was brought to the western United States from Eurasia during the early to mid-1900s, and has since become a common member of many riparian communities in Idaho. We compared leaf chemistry and in-stream processing of Russian olive leaves (exotic) and various species of native leaves in one hardwater and one relatively softwater Idaho stream. Measurements using air-dried leaves showed that Russian olive contained the greatest concentration of nitrogen, approximately 1.6% of the dry mass, whereas the native species each contained less than 1.0% nitrogen. The C/N ratio of Russian olive was 〈30, whereas the natives each had C/N ratios greater than 40. Results from the hardwater stream indicated no difference in 30-day loss of AFDM between Russian olive and the native leaves (dogwood and aspen). In the relatively softwater stream, the Russian olive leaves were processed significantly slower than the native leaf species (cottonwood). The results indicate that a replacement of native riparian trees by exotics, such as Russian olive, may result in slower rates of leaf processing in Idaho streams but that the effect may vary among streams. When comparing the processing of native and exotic leaf litter, initial nitrogen concentrations and initial C/N ratios of the leaves did not appear to be accurate indicators of relative decay rates.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1988), S. 183-183 
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 28 (1993), S. 626-634 
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fragmentation pathways of the synthetic pyrethroid cypermethrin and four structurally related insecticides were investigated using a tandem quadrupole mass spectrometer incorporating a hexapole collision cell under positive-ion electron impact ionization conditions. Conventional mass spectrometry using the first quadrupole analyser only and tandem mass spectrometry on selected precursor ions and product ions, and also constant neutral loss scan experiments, were used. Mechanisms and fragmentation pathways are proposed to explain the inherent stability of ions associated with the benzylphenoxy portion of this class of insecticide.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 4 (1990), S. 436-439 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The introduction of more rigorous regulations aimed at controlling the use of sulphonated dyestuffs and dyestuff intermediates has pointed to the need for screening methods for the presence of these involatile compounds. Although much work has been caried out recently on the mass spectrometric behaviour of these compounds, it has been concerned principally with the production of molecular weight information using novel ionization methods rather than with any attempt to obtain structurally diagnostic fragment ions. In this mass spectrometric investigation of a range of naphthalene sulphonic acid salts, positive-ion and negative-ion fast-atom bombardment (FAB) mass spectra have been obtained and FAB-tandem mass spectrometry (MS/MS) has been used to acquire spectra (daugher-ion, parent-ion and neutral-loss) in an attempt to develop a useful screening method. On known compounds, best results were found when using a negative-ion neutral-loss scan to detect the loss of 64 mass units (corresponding to loss of SO2). This was confirmed by successful use of this scan to screen a sample of sulphonate residues.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 6 (1992), S. 269-271 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: The design and use of an RF-only collision-cell ramp generator is described. The generatio controls the collision energy voltage applied to the RF-only collision cell in a hybrid mass spectrometer of BEqQ geometry. The generator enables the collision-cell voltage to be increased or decreased in a linear ramp, over the full 500 V available. The start/stop functions of the ramp are synchronized under data system control to the scan of the quadrupole mass analyser. The collision-induced dissociation spectra so produced show an increase in the product ion abundance.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1473-1473 
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Type of Medium: Electronic Resource
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