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  • Biology  (30)
  • Identification  (8)
  • Liver  (7)
  • 1
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    Extraction‚ Purification, Identification and amount verification of Steroids, in Sargassum glaucescens and Padina boergesni algae in Oman Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 42pp.
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  • 2
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    The in vivo effect of methyl tert-butyl ether on liver, gills and kidney tissues of Rutilus caspicus (2018)
    Details
    Publication Date: 2021-05-19
    Description: This study was conducted to evaluate histopathological responses in liver, gills and kidney in Rutilus caspicus exposed to concentrations of 50, 100, 150 mg L^-1 of methyl tert-butyl ether, for 7, 14, and 21 days. The experiments were conducted in water temperature of 19±1 °C, dissolved oxygen of 7.6 ± 0.2 mg L^-1 and zero salinity. A total of 156 fish were studied in this experiment. In the first, second and third week of the experiment, three fish were taken randomly from each aquarium. To examine the tissues, the liver, gills and kidney were isolated and prepared for evaluation using standard histological techniques. Tissue damage in the liver includes: blood congestion, congestion of sinusoid, melano macrophage aggregation, hepatocyte hypertrophy, vacuolation, degeneration and cellular necrosis. Gill tissue damage includes: hyperplasia, degeneration lifting, telangiectasis, in secondary lamellae, blood congestion in primary and secondary lamellae, S formation of lamellae, reduction in length of secondary lamella, lamellar fusion and cellular necrosis. Tissue damage in the kidney includes: tubular shrinkage, blood congestion, melano macrophage aggregation, glomerular shrinkage, cellular necrosis, tubular degeneration, reduction in interstsial cells and interstisial hematopoietic tissue degeneration. The amount of tissue damages in high concentrations of pollutants was high, while gill, liver and kidney in the control group was observed in the normal outline. The results of this study showed that methyl tert-butyl ether (MTBE) can cause damage in vital tissues of R. caspicus and even, eventually lead to death.
    Description: Published
    Keywords: Histopathology ; Gill ; Kidney ; Liver ; Methyl tert-butyl ether ; Rutilus caspicus ; In vitro ; MTBE
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.821-834
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  • 3
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    Histopathological effects and toxicity of atrazine herbicide in Caspian Kutum, Rutilus frisii kutum, fry (2018)
    Details
    Publication Date: 2021-05-19
    Description: This study aimed to investigate the toxic effects of atrazine herbicide on the fry of Caspian Kutum (Rutilus frisii kutum, Kamensky, 1901). First the 96-h LC50 of the fry were exposed to atrazine at the concentration of 24.95 ppm was determined. Then the toxicity of this herbicide on Caspian kutum fry exposed to the concentration of 12.47ppm (1/2 LC50), for four days was measured and compared with a control group. Comparison of the length, weight and condition factor showed no significant differences between atrazine exposed and control group. The concentration of Na+, K+, Ca2+, Mg2+ and Cl- in the whole body of fry in control and atrazine exposure groups were as the following order: Ca2+〉K+〉 Na +〉 Cl- 〉Mg2+ and Ca2+〉Na+〉K+〉Mg2+〉Cl-, respectively. Results showed that the concentration of all these ions were higher in atrazine exposure group than control group, except for Cl-, and the only significant differences was found in Na+ concentration. Major histopathological effects of atrazine on the gills were hyperplasia and thickening of the filaments, separation of the pavement cells of the lamellae epithelium from the pillar cells and swelling of the epithelial cells. Results of the present study showed that atrazine could affect the ion composition of the body, and caused major damages in gill epithelium even at sublethal concentration and acute exposure, but had no effects on the growth parameters.
    Description: Published
    Keywords: Biology ; Physiology ; Caspian Kutum ; Atrazine ; Rutilus frisii kutum ; Toxicity ; Ion ; LC50 ; Histopathological ; Growth ; Parameters
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.702-718
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  • 4
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    Histopathological changes of gill, liver and kidney in Caspian kutum exposed to Linear Alkylbenzene Sulfonate (2018)
    Details
    Publication Date: 2021-05-19
    Description: The histopathological effects of Linear Alkylbenzene Sulfonate on the gill, liver and kidney tissues of the Caspian kutum, Rutilus frisii kutum were studied. The fish were exposed to three sublethal concentrations of anionic detergent, Linear Alkylbenzene Sulfonate (LAS) for short term intervals (192 h). Gill, liver and kidney samples were collected after 192 h of exposure to LAS and lesions were analyzed by light microscopy. The histological changes to gills were edema, fusion of lamellae and lamellar aneurism. Some alterations like reduction of the interstitial haematopoietic tissue, tubular shrinkage, degeneration in the epithelial cells of renal tubule and necrosis were observed in the kidney. In the liver tissue, hepatocyte degeneration, congestion and dilation of sinusoid and vacuolar degeneration were seen. It seems that sublethal concentration of LAS may affect sever changes to gill, kidney and liver of R. frisii kutum specimens that leads to malfunction of these organs which cause damage to health of the fish.
    Description: Published
    Keywords: Fish disease ; Caspian kutum ; Rutilus frisii kutum ; Linear Alkylbenzene Sulfonate (LAS) ; Histopathology ; Gill ; Liver ; Kidney
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.887-897
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  • 5
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    Identification of metallothionein gene structure in sterlet (Acipenser ruthenus) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Aquatic organisms present, not only simple sources of accumulated metal, but can interact with metals, altering their toxicity. Due to exposition of biosphere with metals, organisms have developed various defense mechanisms to protect themselves against adverse effects of these ions and their compounds. Metallothionein (MT) is one of that which represents a critical mechanism for detoxification of metals.The sterlet (Acipenser ruthenus) is a bottom feeding sturgeon specie and because the fish are dependent on invertebrate species for food throughout their life cycle, the sterlet could be a good indicator of the quality of the state of water ecosystem. Addition of copper to water leads to the induction of MT. The present study analyzed MTgene that was excreted from the liver of sterlet exposed to sub-lethal copper concentrations (0.075 mgL-1). To begin to elucidate the molecular mechanism(s) of sensitivity of sturgeons to metals, a RNA encoding MT was purified from livers of sterlet, then a cDNA was synthesized and the MTgene was amplified. The primary structure of sterlet metallothionein (S-MT) contained 20 cysteine residues, which is the same as MTs of teleost fishes. However, the primary structure of S-MT contained 63 amino acids, which is longer than any MT identified in teleost fishes but similar to Lake sturgeon and White sturgeon. The complete nucleotide sequence of the S-MTgene has been detected. We have determined the structure of the fish copper-binding protein by DNA sequence analysis of the gene.
    Description: Published
    Keywords: Acipenser ruthenus ; Metallothionein ; Sterlet ; Copper ; Gene structure ; Identification ; Structure ; Gene
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.1267-1277
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  • 6
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    Extraction and Identification steroids in two species marine algae, Sargassum oligocystum and Nizamudiinia zanardinii in Persian Gulf and Oman Sea (2017)
    Details
    Publication Date: 2021-05-19
    Description: Sargassum oligocystum and Nizamudiinia zanardinii are the most abundant algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of S. oligocystum by Chroform-Etanol (3-1) solvent and N. zanardinii by methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC ).. Structural elucidation of the constituents was based on the data obtained from HNMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above algae were identified as 22-dehydrocholesterol (1) cholesterol (2) fucosterol (3) 29-hydroperoxystigmasta-5,24(28)-dien-3β-ol (4) 24-hydroperoxy-24- vinylcholesterol (5) a mixture of 24(S)-hydroxy-24-vinylcholesterol (6) and 24(R)-hydroxy-24- vinylcholesterol (7) and ostreasterol (8) based on their spectral data and from comparison with those previously reported in the literature.
    Description: Published
    Keywords: Sargassum oligocystum ; Nizamudiinia zanardinii ; Algal extract ; Extraction ; Identification ; Steroids ; Species ; Algae ; Marine ; Brown Algae ; Chemical components
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.23-30
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  • 7
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    Effect of Malathion Insecticide on Liver Tissue and Enzymes of Rutilus Rutilus Caspicus of the Caspian Sea (2017)
    Details
    Publication Date: 2021-05-19
    Description: Malathion is an organophosphate insecticide which uses to destroy insects and pests of fruit trees, ornamental plants and agricultural corps.In the present study, effect of Malathion on liver and selected enzymes (SGOT, SGPT and ALP) was studied in Caspian Roach (Rutilusrutiluscaspicus). Four treatments with three replications were designed to carry out the survey. Four groups of experimental fish (containing 30 fish in each group) were exposed to different concentrations of Malathion. e. 0, 0.01, 0.05 and 0.1 ppm respectively for 23 days. Blood collection was done in 3rd , 13th and 23th after exposure to Malathion and also 30 days after recovery in clean water and enzymes were measured using standard kits. Also liver tissues were isolated to histological examination. Results showed that tissues of control group (0ppm) were normal and there were no damages, yet there were hepatocytes degeneration, picnotic in nuclear, hepatocytes vacuolization, vascular congestion and sinusoid congestion in liver of other groups. Tissue damages were increased in higher malathion concentration and over time. Results related to enzymes showed that there were no significant differences in SGOT of fish treated with low concentrations of malathion (0.01 and 0.05 ppm) and control group but it was increased in highest concentration (p〈0.05). Yet, SGPT increased significantly after passing 23 days in all fish exposed to malathion But ALP changes trend was decreasing.
    Description: Published
    Keywords: Malathion ; Liver damage ; Rutilus Rutilus Caspicus ; Caspian Roach ; Enzymes ; Liver ; Tissues
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.117-126
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  • 8
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    Extraction, identification and biological activities (antibacterial, antifungal and cytotoxic) of terpenoid from sponge Dysidea spp. in Hengam Island, Persian gulf for antibiotic and anticancer drugs (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Studies have shown that sponges among aquatic animals produce the most secondary metabolites with bioactivity. This scientific study examines the extraction, identification, antifungal and antibacterial activities of terpenoid and steroid of sponge Dysidea spp. from Hengam Island, Persian Gulf. In this study, the dry powder of the sponge was extracted using acetone solvent by soaking method. Purification of acetone extract was carried out by column chromatography on silica gel with a gradient of n-hexan, ethyl acetate and methanol to isolation of terpenoids. In vitro cytotoxity examined by XTT assay, against KB/ C152, HUT-78/ C185 and Hek293 cell lines, and antimicrobial screening by dilution test examined on bacterial and fungal strains; Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Nocardia brasiliensis and fungal Candida albicans and Aspergillus fumigatus. The components; stigmasterol, phytol, α-Santonin, Ergosta-14,22-don-3-ol,(3β.5α,22E( ،cholest-8(14)-en-3- ol,(3β.5α) and Androstan-17-one,3,11-dimethoxy- 17-methoxime, (3α,5α,11β) were detected by GC/MS. IC50 for stigmasterol, phytol, α-Santonin, Ergosta-14,22-don-3-ol,(3β.5α,22) extracted from Dysidea spp were 67.21 – 103.03 µg/ml in KB cell line and 38.09- 81.18 µg/ml in Hek293 cell line. The results showed phytol extracted from Dysidea spp was active against Gram-positive bacteria Staphylococcus aureus, Bacillus cereus, Bacillus subtilis (MBC 200- 300 µg/ml) and Gram-negetive bacteria Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi (MBC 1000- 2000 µg/ml). The results of this research project show that by performing additional testing, isolating and purifying compounds with biological effects, an effective step can be taken to extract natural compounds in order to produce drugs, cosmetic products, and food.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sgnops ; Natural components ; Biological activity ; Salmonella typhi ; Pseudomonas aeruginosa ; Escherichia coli ; Bacillus subtilis ; Bacillus cereus ; Staphylococcus aureus ; Extraction ; Identification ; Antibiotic ; Anticancer
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 59pp.
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  • 9
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    Extraction and Identification Sterols in Brown alga, Padina boergesenii in Chabahar Coasts (2017)
    Details
    Publication Date: 2021-05-19
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22-dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Description: Published
    Keywords: Brown Algae ; Padina boergesenii ; Steroids compounds ; Algae ; Extraction ; Identification
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.35-44
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  • 10
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    Histopathological effects and toxicity of atrazine herbicide in Caspian kutum, Rutilus frisii kutum, fry (2021)
    In:  http://aquaticcommons.org/id/eprint/22719 | 18721 | 2018-05-17 18:34:08 | 22719 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-09
    Description: This study aimed to investigate the toxic effects of atrazine herbicide on the fry of Caspian kutum (Rutilus frisii kutum, Kamensky, 1901). First the 96-h LC50 of the fry were exposed to atrazine at the concentration of 24.95 ppm was determined. Then the toxicity of this herbicide on Caspian kutum fry exposed to the concentration of 12.47ppm (1/2 LC50), for four days was measured and compared with a control group. Comparison of the length, weight and condition factor showed no significant differences between atrazine exposed and control group. The concentration of Na+, K+, Ca2+, Mg2+ and Cl- in the whole body of fry in control and atrazine exposure groups were as the following order: Ca2+〉K+〉Na+〉Cl-〉Mg2+ and Ca2+〉Na+〉K+〉Mg2+〉Cl-, respectively. Results showed that the concentration of all these ions were higher in atrazine exposure group than control group, except for Cl-, and the only significant differences was found in Na+ concentration. Major histopathological effects of atrazine on the gills were hyperplasia and thickening of the filaments, separation of the pavement cells of the lamellae epithelium from the pillar cells and swelling of the epithelial cells. Results of the present study showed that atrazine could affect the ion composition of the body, and caused major damages in gill epithelium even at sublethal concentration and acute exposure, but had no effects on the growth parameters.
    Keywords: Biology ; Fisheries ; Atrazine ; Rutilus frisii kutum ; Toxicity ; Ion ; LC50 ; Biology ; Physiology ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 702-718
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