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  • Biochemistry and Biotechnology  (1)
  • LDL receptor  (1)
  • Mice  (1)
  • asparagus  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease 1226 (1994), S. 173-180 
    ISSN: 0925-4439
    Keywords: 3-hydroxy-3-methylglutaryl-CoA reductase ; Acyl-CoA ; Cholesterol metabolism ; LDL receptor ; Lymphoblast ; Niemann-Pick disease ; cholesterol acyltransferase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 91 (1995), S. 340-345 
    ISSN: 1432-2242
    Keywords: Selection index ; Restricted index ; Fat ; Mice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this study was to determine if low secondary selection differentials, caused by selecting within full-sib families, may have accounted for the failure of an intended restricted selection index to reduce epididymal fat pad weight (EF) without changing body weight (BW) in mice. Replicate lines that had been selected within full-sib families for high (HE) or low (LE) EF, while holding BW constant, were crossed. After two generations of random mating, two replicates were sampled and selection initiated for the same restricted index criteria except that mass selection was used to increase the selection differentials. In both phases of selection the HE restricted index selection, designed to increase EF without altering BW, was in agreement with expectation. In contrast, the LE index, designed to decrease EF without changing BW, did not agree with theory since BW increased while EF decreased only slightly. Therefore, reduced selection differentials could not explain the deviation from theory. A possible explanation may reside in the restricted selection index being more sensitive to changes in genetic parameters due to shifts in gene frequency as a consequence of the selection applied. However, linkage disequilibrium and genetic drift can not be ruled out as contributing factors to the asymmetry of response.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5036
    Keywords: asparagus ; Asparagus officinalis ; nitrogen fertilizer ; 15N ; plant uptake ; soil nitrogen fractions ; time of application
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of nitrogen (N) fertilizer on the production of a 6-year-old asparagus (Asparagus officinalis L.) crop was examined over 2 years by the application of 0, 50 or 100 kg N ha−1 as ammonium sulphate at three times; 1) prior to fern growth (9 months before harvest), 2) prior to harvest, or 3) early-harvest prior to the main period of spear production. The utilization of N fertilizer was examined by applying 15N-enriched ammonium sulphate to 2 m×2.5 m microplost within the 50 kg N ha−1 treatments. There was a 12% response in spear production to added N in the first harvest year only and there was no significant effect of rate or time of N application. Plant uptake of added 15N by the end of the first harvest period was 25, 11 and 4% of the total applied for the pre-fern, pre-harvest and early-harvest treatments respectively. About 60% of the 15N applied pre- or early-harvest remained in the soil at the end of the first harvest period. Most of the 15N in soil in the pre-harvest treatment occurred as inorganic N and had been leached into the 150–600 mm soil depth. In contrast, most of the 15N that remained in soil from the early-harvest application was in the 0–75 mm depth and 40% of this had been immobilized into organic N. In the short-term (less than one year), utilisation of 15N-labelled N fertilizer by asparagus was greatest when applied prior to fern growth. However, after 2 years there was no difference between treatments in 15N recovery in plant or soil. Thus, time of application of N fertilizer had no long-term effect on production or utilization of N fertilizer by asparagus.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 36 (1990), S. 653-664 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Measurement of the rate of glucose diffusion from EUDGRAGIT RL and HEMA-MMA microcapsules coupled with a Thiele modulus/Biot number analysis of the glucose utilization rate suggests that pancreatic islets and CHO (Chinese hamster ovary) cells (at moderate to high cell densities) should not be adversely affected by the diffusion restrictions associated with these capsule membranes. The mass transfer coefficients for glucose at 20 °C were of the same order of magnitude for both capsules, based on release measurements: ∼5 × 10-6 cm/s for EUDRAGIT RL and ∼2 × 10-6 for HEMA-MMA. Inulin release from EUDRAGIT RL was slower than for glucose (mass transfer coefficient 14 ± 4 × 10-8 cm/s). The Thiele moduli were much less than 1, either for a single islet at the center of a capsule or CHO cells uniformly distributed throughout a capsule at 10-6 cells/ mL, so that diffusion restrictions within the cells in EUDRAGIT RL or 800 μm HEMA-MMA capsules should be negligible. The ratio of external to internal diffusion resistance (Biot number) was less than 1, so that at most, only a small diffusion effect on glucose utilization should be expected (i.e., the overall effectiveness factors were greater than 0.8). These calculations were consistent with experimental observation of encapsulated islet behavior but not fully with CHO cell behavior. Permeability restricted cell viability and growth is potentially a major limitation of encapsulated cells; further analysis is warranted.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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