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  • Astrophysics  (1)
  • Intercellular junctions  (1)
  • Multiphase, Granular, and Particle-Laden Flows  (1)
  • Numerical Methods and Modeling  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 139 (1994), S. 41-48 
    ISSN: 1432-1424
    Keywords: Tight junction ; Intercellular junctions ; Transepithelial permeability ; Transepithelial electrical resistance (TER) ; MDCK, A6, Caco-2 epithelial cell cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We report evidence of a factor secreted at the apical side of epithelial monolayers which modulates tight junction structure and permeability. This activity was detected within 4–7 days of conditioning of the apical medium by MDCK, A6 or Caco-2 epithelial cell lines cultured on permeable membranes in bipartite chambers. Apical conditioned medium (ACM), applied to the basolateral surface of a confluent monolayer, increased the transepithelial electrical resistance (TER), progressively reaching values 12–22% higher than the baseline within 5–10 min. After 40–60 min, the TER returned slowly to the basal value. This phenomenon was not observed either when using preheated ACM or the ACM filtrate obtained through a 30,000 MW cutoff membrane. The ACM maintained its activity even when applied to cell lines from different organs and species, as demonstrated when ACM from MDCK monolayers promoted an increase of 22% in the TER of Caco-2 cells. The increase of TER induced by the ACM treatment is accompanied by a change in the distribution of the number of tight junction strands, from an initial pattern, dominated mostly by junctions with one or two strands, to a new pattern after treatment dominated by junctions with two or three strands. Our results suggest the existence of a mechanism in epithelial cells that could signal leakage of apically secreted components to the basolateral side, thereby modulating the junction structure and permeability.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 28 (1998), S. 719-736 
    ISSN: 0271-2091
    Keywords: front tracking ; mesh adaptation ; finite element method ; Engineering ; Numerical Methods and Modeling
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A numerical model is developed for the simulation of moving interfaces in viscous incompressible flows. The model is based on the finite element method with a pseudo-concentration technique to track the front. Since a Eulerian approach is chosen, the interface is advected by the flow through a fixed mesh. Therefore, material discontinuity across the interface cannot be described accurately. To remedy this problem, the model has been supplemented with a local mesh adaptation technique. This latter consists in updating the mesh at each time step to the interface position, such that element boundaries lie along the front. It has been implemented for unstructured triangular finite element meshes. The outcome of this technique is that it allows an accurate treatment of material discontinuity across the interface and, if necessary, a modelling of interface phenomena such as surface tension by using specific boundary elements. For illustration, two examples are computed and presented in this paper: the broken dam problem and the Rayleigh-Taylor instability. Good agreement has been obtained in the comparison of the numerical results with theory or available experimental data. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2018-07-12
    Description: Author(s): R. Jäger, M. Mendoza, and H. J. Herrmann Using a coupled lattice Boltzmann method/discrete element method model, the clogging of a pore with particles at microscopic scale is studied. We show that erosive bursts occur when the hydraulic pressure exceeds a critical point, which depends on the adhesive forces and the pores’ geometry. [Phys. Rev. Fluids 3, 074302] Published Wed Jul 11, 2018
    Keywords: Multiphase, Granular, and Particle-Laden Flows
    Electronic ISSN: 2469-990X
    Topics: Physics
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  • 4
    Publication Date: 2019-07-17
    Description: We report preliminary results of a massive investigation of the striation patterns observed in the dust tail of comet Hale-Bopp in March and April 1997. Our findings are based on 16 wide-field photographs taken with Schmidt cameras on March 2-20, with six more, from March 31-April 8, still waiting for analysis. Altogether approximately 700 individual striae were examined on the 16 images, which were scanned and computer processed to enhance the morphology. About 5300 stria points, or some 7-8 points per stria per image on the average, were measured and their astrometric positions determined and subsequently converted to a Cartesian coordinate system, aligned with the comet's projected radius vector and centered on the nucleus. The evolution of the striated tail has been studied using the Sekanina-Farrell fragmentation hypothesis (AJ 85, 1538, 1980), previously applied to other comets. This two-step model is characterized by the time of release from the nucleus of a parent object (or objects) whose motion is assumed to have been subjected to a constant repulsive acceleration beta(sub p) (presumably due to solar radiation pressure) until the time of fragmentation.
    Keywords: Astrophysics
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