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  • Common carp  (3)
  • Area  (2)
  • 1
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    A Comparative study of synthetic carp GnRH and carp pituitary homogenate effects on in vitro steroidogenesis of oocytes in common carp (Cyprinus carpio) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Vitellogenic stage carp ovaries were collected aseptically and the cells of ovarian follicles were dispersed by trypsin treatment and cultivated as monolayer. Control cultures were grown in medium M199 alone. Media for experimental cultures were supplemented either with 100 µl ml-1 of CPH and CPS-carp GnRH (cultivated pituitary cell secretion affected by 10-6, 10-8, 10-10 M carp GnRH). The direct effect of these factors on 17-α-Hydroxy progesterone (17α -OHP) and 17β-Estradiol (E2) secretion of isolated follicular cells in cell culture was assayed by appropriate Radioimmunoassay (RIA). According to the results the E2 was a main hormone secreted by cultivated carp granulosa and theca cells. Adding 100 µl ml-1of 10-6 M CPS-carp GnRH had no significant effect on cultivated granulosa and theca cells endocrine activity (P〉 0.05). On the other hand, carp granulosa and theca cells treated with the lower contents of CPS-carp GnRH (10-8, 10 -10 M) showed a 4.02 and 3.74-fold increase in E2 concentration and 4.27 and 3.64-fold increase in 17α -OHP level, which was significantly higher than CPH (P〈 0.05). Also, there was a significant increase of steroid secretion in group stimulated with 100 µl ml-1 of CPS and CPH, but there were no significant differences between them (P〉 0.05).
    Description: Published
    Keywords: Common carp ; Control cultures ; Synthetic ; Cyprinus carpio ; Cell culture ; Carp GnRH ; Gonadotropin hormones ; Steroid hormones ; Homogenate ; Steroidogenesis
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.394-404
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  • 2
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    Effects of total protein and cholesterol on spermatology traits of common carp (Cyprinus carpio) (2021)
    In:  http://aquaticcommons.org/id/eprint/25031 | 18721 | 2019-01-07 08:03:35 | 25031 | Khorramshahr University of Marine Science and Technology
    Details
    Publication Date: 2021-07-16
    Description: Seminal plasma has contained organics factors same as proteins and cholesterol. The information about role and variety of fish seminal plasma and relationship with other factors especially in common carp is rare. The aim of this study was evaluation of effects of total protein and cholesterol on physical and biochemical factors of cultural common carp milt in Khouzestan province. For this study 40 fish have been bought (20 in March and 20 in April) and examinations of physical and biochemical were tested. Base on the results, total protein in March and April was 0.29±0.14 and 0.37±0.35 g/dl respectively, cholesterol in March and April was 9/95±8.83 and 14/12±12.73 mg/dl respectively. The total protein and cholesterol in April compared to March increased non significantly. The ions of calcium, chloride, sodium and potassium in April compared to March increased. Based on the results there was significant and positive correlation between total protein and Albumin, cholesterol and K in march and significant and negative correlation with Cl but total protein in April had significant and positive with Cl, P, spermatocrite, cholesterol and Albumin and negative and significant correlation with weight of fish. Cholesterol in March had significant and positive with albumin and negative significant correlation with Cl and P but cholesterol in April had positive significant correlation with spermatocrite, Albumin, P and Cl. According to the data of this study, total protein, cholesterol, calcium, chloride, sodium, potassium of the Common carp seminal plasma increased in April compared to March. So because of these factors have effects on fertility, quality and quantity of common carp sperm so it is possible to adjust the parameters of protein, cholesterol and minerals of dietary in spawning season increase the quality of common carp sperm.
    Keywords: Biology ; Fisheries ; Iran ; Common carp ; Total protein ; Cholesterol ; Cyprinus carpio ; Spermatology ; Protein ; Biochemical ; Physical ; Quality ; Quantity ; Sperm
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 66-75
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  • 3
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    Effect of different salinity concentration on different areas of the body skin of benni, Barbuss harpeyi (2021)
    In:  http://aquaticcommons.org/id/eprint/25059 | 18721 | 2018-11-16 08:47:13 | 25059 | Khorramshahr University of Marine Science and Technology
    Details
    Publication Date: 2021-07-16
    Description: For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition. In days (1, 3, 7, 14, 21, 28) samples were collected from skin of head, ventral and lateral lines. Sections with 5- 6µm thickness were made and stained with PAS and H&E methods. In histometrical survey, epiderm thickness, numbering of club cells and goblet cells in 200 µm of epiderm length in any area, was investigated comparatively. The results showed that in different salinity concentrations, skin of head, ventral and lateral lines were influenced and changes in epiderm thickness, reduce the number of club cells and increase the number of goblet cells were demonstrated. These changes in different concentrations for each parameter, for significantly increased (p〈0.05).
    Keywords: Biology ; Ecology ; Iran ; Salinity ; Concentration ; Area ; Body ; Barbuss harpeyi ; Skin ; Epiderm ; Histometrical ; Survey
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 89-101
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  • 4
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    Effects of total protein and cholesterol on spermatology traits of common carp (Cyprinus carpio) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Seminal plasma has contained organics factors same as proteins and cholesterol. The information about role and variety of fish seminal plasma and relationship with other factors especially in common carp is rare. The aim of this study was evaluation of effects of total protein and cholesterol on physical and biochemical factors of cultural common carp milt in Khouzestan province. For this study 40 fish have been bought (20 in March and 20 in April) and examinations of physical and biochemical were tested. Base on the results, total protein in March and April was 0.29±0.14 and 0.37±0.35 g/dl respectively, cholesterol in March and April was 9/95±8.83 and 14/12±12.73 mg/dl respectively. The total protein and cholesterol in April compared to March increased nonsignificantly. The ions of calcium, chloride, sodium and potassium in April compared to March increased. Based on the results there was significant and positive correlation between total protein and Albumin, cholesterol and K in march and significant and negative correlation with Cl but total protein in April had significant and positive with Cl, P, spermatocrit, cholesterol and Albumin and negative and significant correlation with weight of fish. Cholesterol in March had significant and positive with albumin and negative significant correlation with Cl and P but cholesterol in April had positive significant correlation with spermatocrit, Albumin, P and Cl. According to the data of this study, total protein, cholesterol, calcium, chloride, sodium, potassium of the Common carp seminal plasma increased in April compared to March. So because of these factors have effects on fertility, quality and quantity of common carp sperm So it is possible to adjust the parameters of protein, cholesterol and minerals of dietary in spawing season increase the quality of common carp sperm.
    Description: Published
    Keywords: Common carp ; Total protein ; Cholesterol ; Cyprinus carpio ; Spermatology ; Protein ; Biochemical ; Physical ; Quality ; Quantity ; Sperm
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.66-75
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  • 5
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    Effect of different salinity concentration on different areas of the body skin of benni, Barbuss harpeyi (2018)
    Details
    Publication Date: 2021-11-22
    Description: For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition . In days (1, 3, 7, 14, 21, 28) samples were collected from skin of head, ventral and lateral lines. Sections with 5- 6µm thickness were made and stained with PAS and H&E methods. In histometrical survey, epiderm thickness, numbering of club cells and goblet cells in 200 µm of epiderm length in any area, was investigated comparatively. The results showed that in different salinity concentrations, skin of head, ventral and lateral lines were influenced and changes in epiderm thickness, reduce the number of club cells and increase the number of goblet cells were demonstrated. These changes in different concentrations for each parameter, for significantly increased (p〈0.05).
    Description: Published
    Keywords: Salinity ; Concentration ; Area ; Body ; Barbuss harpeyi ; Skin ; Epiderm ; Histometrical ; Survey
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.89-101
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