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  • Common carp  (3)
  • Hematology  (3)
  • Area  (2)
  • 1
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    Effects of Aloe vera extract on growth and some hematological parameters of shirbot, Tor grypus (Heckel, 1843) (2020)
    Details
    Publication Date: 2021-05-19
    Description: A feeding trial was conducted for sixty days to determine the effect of dietary Aloe vera on parameters related to growth rate, health status and hematological parameters of shirbot (Tor grypus). Two hundred and forty fish weighing 50-60 g were randomly subjected to four different treatments, including a control, T1 (0.1% of A. vera), T2 (0.2%) and T3 (0.5%) in triplicate. Hematological parameters and some growth parameters, including the body weight, total length, condition factor, feed conversion ratio, feed efficiency, specific growth rate and protein efficiency ratio were measured. Administration of fish to different concentrations of A. vera extract led to significant (p〈0.05) increase in total erythrocyte count, packed cell volume, and hemoglobin when compared with the control group. Mean corpuscular hemoglobin concentration were increased only in T2 and T3 in comparison with the control. When animals received 5% of A. vera daily, they showed a significant increase (29%) in white blood cells (p〈0.05). Growth parameters were improved with the addition of different concentrations of A. vera to fish food. According to the results obtained, it might be concluded that feeding this species with A. vera extract could likely enhance growth rate and also hematological parameters.
    Description: Published
    Keywords: Aloe vera ; Tor grypus ; Growth parameters ; Hematology ; Feeding ; Dietary ; Fish ; Health
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.445-456
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  • 2
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    A Comparative study of synthetic carp GnRH and carp pituitary homogenate effects on in vitro steroidogenesis of oocytes in common carp (Cyprinus carpio) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Vitellogenic stage carp ovaries were collected aseptically and the cells of ovarian follicles were dispersed by trypsin treatment and cultivated as monolayer. Control cultures were grown in medium M199 alone. Media for experimental cultures were supplemented either with 100 µl ml-1 of CPH and CPS-carp GnRH (cultivated pituitary cell secretion affected by 10-6, 10-8, 10-10 M carp GnRH). The direct effect of these factors on 17-α-Hydroxy progesterone (17α -OHP) and 17β-Estradiol (E2) secretion of isolated follicular cells in cell culture was assayed by appropriate Radioimmunoassay (RIA). According to the results the E2 was a main hormone secreted by cultivated carp granulosa and theca cells. Adding 100 µl ml-1of 10-6 M CPS-carp GnRH had no significant effect on cultivated granulosa and theca cells endocrine activity (P〉 0.05). On the other hand, carp granulosa and theca cells treated with the lower contents of CPS-carp GnRH (10-8, 10 -10 M) showed a 4.02 and 3.74-fold increase in E2 concentration and 4.27 and 3.64-fold increase in 17α -OHP level, which was significantly higher than CPH (P〈 0.05). Also, there was a significant increase of steroid secretion in group stimulated with 100 µl ml-1 of CPS and CPH, but there were no significant differences between them (P〉 0.05).
    Description: Published
    Keywords: Common carp ; Control cultures ; Synthetic ; Cyprinus carpio ; Cell culture ; Carp GnRH ; Gonadotropin hormones ; Steroid hormones ; Homogenate ; Steroidogenesis
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.394-404
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  • 3
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    Haemato-immunological responses to diazinon chronic toxicity in Barbus sharpeyi (2018)
    Details
    Publication Date: 2021-05-19
    Description: The present research aimed to determine acute toxicity and evaluate the effect of sub-lethal concentrations of diazinon on some haematological and immunological parameters of Barbus sharpeyi by the Organization for Economic Cooperation and Development methods. Juvenile B. sharpeyi were exposed to 0, 5%, 10% and 25% 96h LC50 concentrations of diazinon for 21 days. Blood samples were taken after 7, 14 and 21 days of exposure to diazinon. Haematological and biochemical parameters including: RBC, Hb, PCV and WBC, as well as serum enzymes were evaluated. Immunological indices and resistance against bacterial infection, were determined. Results showed that 96h LC50 of diazinon in B. sharpeyi was estimated at 3.987 mg/L. Diazinon toxicity at a level of 1 and 0.4 mg/L decreased RBC, Hb, and Hematocrite, in almost all sampling periods compared. WBC and globular index decreased significantly in fish exposed to 1 mg/L diazinon on days 14 and 21(p〈0.05). Dose dependent increase in serum enzymes were seen in fish exposed to diazinon. Diazinon toxicity showed no effect on serum LDH level (p〉0.05). Dose dependent decrease in serum total protein and globulin were indicated in diazinon exposed fish. Serum lysozyme and bactericidal activity decreased in T3 and T4 at days 14 and 21. Mortality following challenge to A.hydrophila increased in fish exposed to 1 mg/l diazinon. It can be concluded that diazinon is toxic to B. sharpeyi. Therefore, the strict biosecurity should be taken into consideration when this pesticide is used in agricultural fields surrounding freshwater sources of fish cultivation
    Description: Published
    Keywords: Biology ; Physiology ; Diazinon ; Toxicity ; Barbus sharpeyi ; Immunological parameters ; Hematology
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.870-885
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  • 4
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    Effects of total protein and cholesterol on spermatology traits of common carp (Cyprinus carpio) (2021)
    In:  http://aquaticcommons.org/id/eprint/25031 | 18721 | 2019-01-07 08:03:35 | 25031 | Khorramshahr University of Marine Science and Technology
    Details
    Publication Date: 2021-07-16
    Description: Seminal plasma has contained organics factors same as proteins and cholesterol. The information about role and variety of fish seminal plasma and relationship with other factors especially in common carp is rare. The aim of this study was evaluation of effects of total protein and cholesterol on physical and biochemical factors of cultural common carp milt in Khouzestan province. For this study 40 fish have been bought (20 in March and 20 in April) and examinations of physical and biochemical were tested. Base on the results, total protein in March and April was 0.29±0.14 and 0.37±0.35 g/dl respectively, cholesterol in March and April was 9/95±8.83 and 14/12±12.73 mg/dl respectively. The total protein and cholesterol in April compared to March increased non significantly. The ions of calcium, chloride, sodium and potassium in April compared to March increased. Based on the results there was significant and positive correlation between total protein and Albumin, cholesterol and K in march and significant and negative correlation with Cl but total protein in April had significant and positive with Cl, P, spermatocrite, cholesterol and Albumin and negative and significant correlation with weight of fish. Cholesterol in March had significant and positive with albumin and negative significant correlation with Cl and P but cholesterol in April had positive significant correlation with spermatocrite, Albumin, P and Cl. According to the data of this study, total protein, cholesterol, calcium, chloride, sodium, potassium of the Common carp seminal plasma increased in April compared to March. So because of these factors have effects on fertility, quality and quantity of common carp sperm so it is possible to adjust the parameters of protein, cholesterol and minerals of dietary in spawning season increase the quality of common carp sperm.
    Keywords: Biology ; Fisheries ; Iran ; Common carp ; Total protein ; Cholesterol ; Cyprinus carpio ; Spermatology ; Protein ; Biochemical ; Physical ; Quality ; Quantity ; Sperm
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 66-75
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  • 5
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    Haemato-immunological responses to diazinon chronic toxicity in Barbus sharpeyi (2021)
    In:  http://aquaticcommons.org/id/eprint/22919 | 18721 | 2018-06-06 01:50:03 | 22919 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-11
    Description: The present research aimed to determine acute toxicity and evaluate the effect of sub-lethal concentrations of diazinon on some haematological and immunological parameters of Barbus sharpeyi by the Organization for Economic Cooperation and Development methods. Juvenile B. sharpeyi were exposed to 0, 5%, 10% and 25% 96h LC50 concentrations of diazinon for 21 days. Blood samples were taken after 7, 14 and 21 days of exposure to diazinon. Haematological and biochemical parameters including: RBC, Hb, PCV and WBC, as well as serum enzymes were evaluated. Immunological indices and resistance against bacterial infection, were determined. Results showed that 96h LC50 of diazinon in B. sharpeyi was estimated at 3.987 mg/L. Diazinon toxicity at a level of 1 and 0.4 mg/L decreased RBC, Hb, and Hematocrite, in almost all sampling periods compared. WBC and globular index decreased significantly in fish exposed to 1 mg/L diazinon on days 14 and 21(p〈0.05). Dose dependent increase in serum enzymes were seen in fish exposed to diazinon. Diazinon toxicity showed no effect on serum LDH level (p〉0.05). Dose dependent decrease in serum total protein and globulin were indicated in diazinon exposed fish. Serum lysozyme and bactericidal activity decreased in T3 and T4 at days 14 and 21. Mortality following challenge to A. hydrophila increased in fish exposed to 1 mg/l diazinon. It can be concluded that diazinon is toxic to B. sharpeyi. Therefore, the strict biosecurity should be taken into consideration when this pesticide is used in agricultural fields surrounding freshwater sources of fish cultivation.
    Keywords: Biology ; Chemistry ; Fisheries ; Pollution ; Diazinon ; Toxicity ; Barbus sharpeyi ; Immunological parameters ; Hematology ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 870-885
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  • 6
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    Effect of different salinity concentration on different areas of the body skin of benni, Barbuss harpeyi (2021)
    In:  http://aquaticcommons.org/id/eprint/25059 | 18721 | 2018-11-16 08:47:13 | 25059 | Khorramshahr University of Marine Science and Technology
    Details
    Publication Date: 2021-07-16
    Description: For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition. In days (1, 3, 7, 14, 21, 28) samples were collected from skin of head, ventral and lateral lines. Sections with 5- 6µm thickness were made and stained with PAS and H&E methods. In histometrical survey, epiderm thickness, numbering of club cells and goblet cells in 200 µm of epiderm length in any area, was investigated comparatively. The results showed that in different salinity concentrations, skin of head, ventral and lateral lines were influenced and changes in epiderm thickness, reduce the number of club cells and increase the number of goblet cells were demonstrated. These changes in different concentrations for each parameter, for significantly increased (p〈0.05).
    Keywords: Biology ; Ecology ; Iran ; Salinity ; Concentration ; Area ; Body ; Barbuss harpeyi ; Skin ; Epiderm ; Histometrical ; Survey
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 89-101
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  • 7
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    Effects of total protein and cholesterol on spermatology traits of common carp (Cyprinus carpio) (2018)
    Details
    Publication Date: 2021-05-19
    Description: Seminal plasma has contained organics factors same as proteins and cholesterol. The information about role and variety of fish seminal plasma and relationship with other factors especially in common carp is rare. The aim of this study was evaluation of effects of total protein and cholesterol on physical and biochemical factors of cultural common carp milt in Khouzestan province. For this study 40 fish have been bought (20 in March and 20 in April) and examinations of physical and biochemical were tested. Base on the results, total protein in March and April was 0.29±0.14 and 0.37±0.35 g/dl respectively, cholesterol in March and April was 9/95±8.83 and 14/12±12.73 mg/dl respectively. The total protein and cholesterol in April compared to March increased nonsignificantly. The ions of calcium, chloride, sodium and potassium in April compared to March increased. Based on the results there was significant and positive correlation between total protein and Albumin, cholesterol and K in march and significant and negative correlation with Cl but total protein in April had significant and positive with Cl, P, spermatocrit, cholesterol and Albumin and negative and significant correlation with weight of fish. Cholesterol in March had significant and positive with albumin and negative significant correlation with Cl and P but cholesterol in April had positive significant correlation with spermatocrit, Albumin, P and Cl. According to the data of this study, total protein, cholesterol, calcium, chloride, sodium, potassium of the Common carp seminal plasma increased in April compared to March. So because of these factors have effects on fertility, quality and quantity of common carp sperm So it is possible to adjust the parameters of protein, cholesterol and minerals of dietary in spawing season increase the quality of common carp sperm.
    Description: Published
    Keywords: Common carp ; Total protein ; Cholesterol ; Cyprinus carpio ; Spermatology ; Protein ; Biochemical ; Physical ; Quality ; Quantity ; Sperm
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.66-75
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  • 8
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    Effect of different salinity concentration on different areas of the body skin of benni, Barbuss harpeyi (2018)
    Details
    Publication Date: 2021-11-22
    Description: For this purpose, 144 specimens were studied in five healthy groups. The first group was the control group put in dechlorinated tap water and four respectively in the next group were kept with salinity 4ppt, 8ppt, 12ppt, 16ppt in the same condition . In days (1, 3, 7, 14, 21, 28) samples were collected from skin of head, ventral and lateral lines. Sections with 5- 6µm thickness were made and stained with PAS and H&E methods. In histometrical survey, epiderm thickness, numbering of club cells and goblet cells in 200 µm of epiderm length in any area, was investigated comparatively. The results showed that in different salinity concentrations, skin of head, ventral and lateral lines were influenced and changes in epiderm thickness, reduce the number of club cells and increase the number of goblet cells were demonstrated. These changes in different concentrations for each parameter, for significantly increased (p〈0.05).
    Description: Published
    Keywords: Salinity ; Concentration ; Area ; Body ; Barbuss harpeyi ; Skin ; Epiderm ; Histometrical ; Survey
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.89-101
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