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  • Archaeol, per cell; Carbon, organic, total, per cell; Dry mass; Glycerol dialkyl glycerol tetraether, per cell; Growth phase; Incubation duration; Nitrogen, total, per cell; Phosphorus, total, per cell; Replicate; Thermococcus kodakarensis; Treatment  (1)
  • Genome stabilization  (1)
  • Key words Hyperthermophilic  (1)
  • Methanococcus  (1)
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Keywords
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  • 1
    Electronic Resource
    Electronic Resource
    Archaeal histone stability, DNA binding, and transcription inhibition above 90°C (1998)
    Springer
    Extremophiles 2 (1998), S. 75-81 
    Details
    ISSN: 1433-4909
    Keywords: Key words Protein stability ; Salt dependence ; Genome stabilization ; In vitro transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The DNA binding and compacting activities of the recombinant (r) archaeal histones rHMfA and rHMfB from Methanothermus fervidus, and rHPyA1 from Pyrococcus species GB-3a, synthesized in Escherichia coli, have been shown to be completely resistant to incubation for 4 h at 95°C in the presence of 1 M KCl. Continued incubation of rHMfA and rHMfB at 95°C resulted in a gradual loss of these activities, and rHMfA and rHMfB lost activity more rapidly at 95°C when the salt environment was reduced to 200 mM KCl. rHPyA1, in contrast, retained full activity even after a 60-h incubation at 95°C in 1 M KCl, and reducing the salt concentration did not affect the heat resistance of rHPyA1. rHPyA1–DNA complexes remained intact at 100°C, and rHPyA1 bound to the template DNA in in vitro transcription reaction mixtures assembled using Pyrococcus furiosus components at 90°C. Transcription in vitro from the P. furiosus gdh promoter was reduced by rHPyA1 binding, in a manner that was dependent on the histone-to-DNA ratio and on the topology of the DNA template. Transcription from circular templates was more sensitive to rHPyA1 binding than transcription from a linear template, consistent with rHPyA1 binding introducing physical barriers to transcription and causing changes in the topology of circular templates that also reduced transcription.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050045
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cell-free transcription of the nifH1 gene of Methanococcus thermolithotrophicus indicates that promoters of archaeal nif genes share basic features with the methanogen consensus promoter (1992)
    Springer
    Molecular genetics and genomics 231 (1992), S. 286-295 
    Details
    ISSN: 1617-4623
    Keywords: Archaea ; Nitrogen fixation ; nifH promoter ; Cell-free transcription ; Methanococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nifH1 gene of Methanococcus thermolithotrophicus, which encodes the putative dinitrogenase reductase of an archaeon, was accurately transcribed in a homologous cell-free transcription system. Extracts of cells grown with N2 or ammonia as nitrogen source initiated transcription at the nifH1 promoter with similar efficiencies. We confirmed that cells grown under non-N2-fixing conditions do not contain significant amounts of nifH1-specific mRNA. The levels of cell-free transcription initiation at the nifH1 promoter wer similar to those observed at a tRNA promoter. The DNA sequence from −40 to +5 relative to the initiator nucleotide of nifH1 mRNA contained all the information required for promoter activity. A mutational analysis of this section of DNA demonstrated that a TATA box at −25 and the TTGT motif (initiator element) at the transcription start site are essential for cell-free transcription. These elements are similar to the structural determinants of a known tRNA promoter of Methanococcus. Mutation of a sequence, showing homology to the bacterial NifA site, which overlaps the transcription start site, did not affect promoter activity. Hence, cell-free transcription of the Methanococcus nifH1 gene is independent of upstream activator elements and does not require alternate cis-acting sequences that differ from the methanogen consensus promoter. These findings suggest that the activation of nif promoters is brought about by fundamentally different mechanisms in Archaea and bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279802
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  • 3
    Electronic Resource
    Electronic Resource
    Stetteria hydrogenophila, gen. nov. and sp. nov., a novel mixotrophic sulfur-dependent crenarchaeote isolated from Milos, Greece (1997)
    Springer
    Extremophiles 1 (1997), S. 67-73 
    Details
    ISSN: 1433-4909
    Keywords: Key words Hyperthermophilic ; Crenarchaeota ; Hydrogen and sulfur dependence ; Hydrothermal ; Stetteria hydrogenophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new hyperthermophilic, strictly anaerobic crenarchaeote, Stetteria hydrogenophila DSM11227 representing a new genus within the family of Desulfurococcaceae, was isolated from the sediment of a marine hydrothermal system at Paleohori Bay in Milos, Greece. Cells are gram-negative irregular and disc-shaped cocci, 0.5–1.5 μm in diameter, which are flagellate and can form cytoplasmatic protrusions up to 2 μm in length. The strain grew optimally at 95°C at pH 6.0 and at a NaCl concentration of 3%. The organism grew mixotrophically on peptide substrates. It required elemental sulfur as an external electron acceptor, and in addition, its growth was completely dependent on the presence of molecular hydrogen. Sulfur could be replaced by thiosulfate. H2S, CO2, acetate, and ethanol were identified as products of metabolism. The G + C content of DNA was 65 mol%. Analysis of its phylogenetic position by sequence analysis of 16S rRNA placed this organism in the family of Desulfurococcaceae. The dependence of this organism on both hydrogen and sulfur during growth on peptide substrates distinguishes Stetteria from all previously described species of Crenarchaeota.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050016
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  • 4
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    Polar membrane lipids and elemental composition of Thermococcus kodakarensis cells according to growth stage and phosphorus supply (2014)
    PANGAEA
    In:  Supplement to: Meador, Travis B; Gagen, Emma; Loscar, Michael E; Goldhammer, Tobias; Yoshinaga, Marcos Yukio; Wendt, Jenny; Thomm, Michael; Hinrichs, Kai-Uwe (2014): Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability. Frontiers in Microbiology, 5, 1-13, https://doi.org/10.3389/fmicb.2014.00010
    Details
    Publication Date: 2024-03-07
    Description: We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2-8%) compared to control cells (〈0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria.
    Keywords: Archaeol, per cell; Carbon, organic, total, per cell; Dry mass; Glycerol dialkyl glycerol tetraether, per cell; Growth phase; Incubation duration; Nitrogen, total, per cell; Phosphorus, total, per cell; Replicate; Thermococcus kodakarensis; Treatment
    Type: Dataset
    Format: text/tab-separated-values, 458 data points
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    DATA PANGAEA
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