Publikationsdatum:
2010-04-23
Beschreibung:
DNA methylation is an important epigenetic mark in many eukaryotes. In plants, 24-nucleotide small interfering RNAs (siRNAs) bound to the effector protein, Argonaute 4 (AGO4), can direct de novo DNA methylation by the methyltransferase DRM2 (refs 2, 4-6). Here we report a new regulator of RNA-directed DNA methylation (RdDM) in Arabidopsis: RDM1. Loss-of-function mutations in the RDM1 gene impair the accumulation of 24-nucleotide siRNAs, reduce DNA methylation, and release transcriptional gene silencing at RdDM target loci. RDM1 encodes a small protein that seems to bind single-stranded methyl DNA, and associates and co-localizes with RNA polymerase II (Pol II, also known as NRPB), AGO4 and DRM2 in the nucleus. Our results indicate that RDM1 is a component of the RdDM effector complex and may have a role in linking siRNA production with pre-existing or de novo cytosine methylation. Our results also indicate that, although RDM1 and Pol V (also known as NRPE) may function together at some RdDM target sites in the peri-nucleolar siRNA processing centre, Pol II rather than Pol V is associated with the RdDM effector complex at target sites in the nucleoplasm.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865564/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2865564/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gao, Zhihuan -- Liu, Hai-Liang -- Daxinger, Lucia -- Pontes, Olga -- He, Xinjian -- Qian, Weiqiang -- Lin, Huixin -- Xie, Mingtang -- Lorkovic, Zdravko J -- Zhang, Shoudong -- Miki, Daisuke -- Zhan, Xiangqiang -- Pontier, Dominique -- Lagrange, Thierry -- Jin, Hailing -- Matzke, Antonius J M -- Matzke, Marjori -- Pikaard, Craig S -- Zhu, Jian-Kang -- R01 GM059138/GM/NIGMS NIH HHS/ -- R01 GM059138-12/GM/NIGMS NIH HHS/ -- R01 GM070795/GM/NIGMS NIH HHS/ -- R01 GM070795-06/GM/NIGMS NIH HHS/ -- R01 GM070795-06S1/GM/NIGMS NIH HHS/ -- R01 GM077590/GM/NIGMS NIH HHS/ -- R01 GM093008/GM/NIGMS NIH HHS/ -- England -- Nature. 2010 May 6;465(7294):106-9. doi: 10.1038/nature09025. Epub 2010 Apr 21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California, Riverside, California 92521, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20410883" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Arabidopsis/*metabolism
;
Arabidopsis Proteins/genetics/*metabolism
;
Argonaute Proteins
;
Cell Nucleus/metabolism
;
*DNA Methylation
;
DNA-Binding Proteins/genetics/*metabolism
;
Gene Expression Regulation, Plant
;
Gene Silencing/physiology
;
Methyltransferases/metabolism
;
Mutation
;
RNA Polymerase II/*metabolism
;
RNA, Plant/*metabolism
Print ISSN:
0028-0836
Digitale ISSN:
1476-4687
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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