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    Resetting central and peripheral circadian oscillators in transgenic rats (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2000-04-28
    Description: In multicellular organisms, circadian oscillators are organized into multitissue systems which function as biological clocks that regulate the activities of the organism in relation to environmental cycles and provide an internal temporal framework. To investigate the organization of a mammalian circadian system, we constructed a transgenic rat line in which luciferase is rhythmically expressed under the control of the mouse Per1 promoter. Light emission from cultured suprachiasmatic nuclei (SCN) of these rats was invariably and robustly rhythmic and persisted for up to 32 days in vitro. Liver, lung, and skeletal muscle also expressed circadian rhythms, which damped after two to seven cycles in vitro. In response to advances and delays of the environmental light cycle, the circadian rhythm of light emission from the SCN shifted more rapidly than did the rhythm of locomotor behavior or the rhythms in peripheral tissues. We hypothesize that a self-sustained circadian pacemaker in the SCN entrains circadian oscillators in the periphery to maintain adaptive phase control, which is temporarily lost following large, abrupt shifts in the environmental light cycle.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yamazaki, S -- Numano, R -- Abe, M -- Hida, A -- Takahashi, R -- Ueda, M -- Block, G D -- Sakaki, Y -- Menaker, M -- Tei, H -- MH56647/MH/NIMH NIH HHS/ -- R01 MH056647/MH/NIMH NIH HHS/ -- New York, N.Y. -- Science. 2000 Apr 28;288(5466):682-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉NSF Center for Biological Timing and Department of Biology, University of Virginia, Charlottesville, VA 22903-2477, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10784453" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Animals, Genetically Modified ; Biological Clocks/*physiology ; Cell Cycle Proteins ; Circadian Rhythm/*physiology ; Culture Techniques ; Darkness ; Genes, Reporter ; Light ; Liver/physiology ; Luciferases/genetics/metabolism ; Lung/physiology ; Male ; Mice ; Motor Activity ; Muscle, Skeletal/physiology ; Nuclear Proteins/genetics/physiology ; Period Circadian Proteins ; Promoter Regions, Genetic ; Rats ; Suprachiasmatic Nucleus/*physiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Single-molecule analysis of chemotactic signaling in Dictyostelium cells (2001)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2001-10-27
    Description: Single-molecule imaging techniques were used to reveal the binding of individual cyclic adenosine 3',5'-monophosphate molecules to heterotrimeric guanine nucleotide-binding protein coupled receptors on the surface of living Dictyostelium discoideum cells. The binding sites were uniformly distributed and diffused rapidly in the plane of the membrane. The probabilities of individual association and dissociation events were greater for receptors at the anterior end of the cell. Agonist-induced receptor phosphorylation had little effect on any of the monitored properties, whereas G protein coupling influenced the binding kinetics. These observations illustrate the dynamic properties of receptors involved in gradient sensing and suggest that these may be polarized in chemotactic cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ueda, M -- Sako, Y -- Tanaka, T -- Devreotes, P -- Yanagida, T -- New York, N.Y. -- Science. 2001 Oct 26;294(5543):864-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Recognition and Formation, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Corporation (JST)., Osaka 562-0035, Japan. ueda@phys1.med.osaka-u.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11679673" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Carbocyanines/metabolism ; Cell Membrane/metabolism ; *Chemotaxis ; Cyclic AMP/*metabolism ; Dictyostelium/cytology/genetics/metabolism/*physiology ; Diffusion ; Guanosine Diphosphate/pharmacology ; Guanosine Triphosphate/pharmacology ; Heterotrimeric GTP-Binding Proteins/genetics/*metabolism ; Kinetics ; Microscopy, Fluorescence ; Mutation ; Phosphorylation ; Pseudopodia/metabolism ; Receptors, Cyclic AMP/*metabolism ; *Signal Transduction
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
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