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  • 1
    Electronic Resource
    Electronic Resource
    On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae (1983)
    Springer
    Archives of microbiology 136 (1983), S. 131-136 
    Details
    ISSN: 1432-072X
    Keywords: Redox control ; Enterobacteriaceae ; Anaerobiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutants of Escherichia coli were isolated in which transcription of the structural genes for hydrogenase (hyd) and for one of the components of formate dehydrogenase (fdh) (of the formate hydrogen-lyase complex) is coupled with that of the lacZ gene. They were — together with lac fusions of the nifH and nifL genes from Klebsiella — used to study regulation by redox control, of the expression of the respective structural genes. The following results were obtained: (i) β-galactosidase synthesis was fully repressed in the presence of O2 or nitrate (anaerobically), and induced in the absence of an external electron acceptor. Fumarate as terminal electron acceptor only marginally affected nif expression and partially repressed hyd and fdh expression. Redox control of the synthesis of hydrogenase and formate dehydrogenase, therefore, (as well as that of nif) acts at the level of transcription; the size of the redox potential seems to be correlated with the amount of repression; (ii) β-galactosidase synthesis in the hyd:: lac and fdh::lac fusion strains is induced by formate. At high concentrations formate reverses the repression by nitrate and fumarate but not that by oxygen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404787
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of pyruvate formate-lyase of Escherichia coli from the cloned structural gene (1982)
    Springer
    Archives of microbiology 132 (1982), S. 365-371 
    Details
    ISSN: 1432-072X
    Keywords: Pyruvate formate-lyase ; Anaerobiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is shown here that a plasmid (p29) derived from the transducing phage λaspC2 (Christiansen and Pedersen 1981) codes for pyruvate formate-lyase. The identity of the 80 kilodaltons (kd) gene product of plasmid p29 with the pyruvate formate-lyase polypeptide was proven (i) by comigration of the gene product expressed in the maxicell system with purified enzyme on O'Farrell gels, and (ii) by comparison of the peptide maps obtained from limited proteolysis. In vivo the 80 kd form of the enzyme was proteolytically converted to a 78 kd polypeptide. The two polypeptides (80 kd and 78 kd) and their charge isomers present in purified enzyme preparations are therefore products of a single gene. Aerobically grown cells of Escherichia coli contained a basal level of pyruvate formate-lyase which was derepressed 5-to 10-fold under anaerobiosis. Derepression also occurred during anaerobic growth on glycerol plus fumarate. Presence of plasmid p29 caused overproduction of pyruvate formatelyase, 11-fold upon anaerobic growth on glucose, 14-fold upon aerobic growth on glucose and 33-fold upon aerobic growth at the expense of D-lactate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413390
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  • 3
    Electronic Resource
    Electronic Resource
    Partial suppression of cell mediated immunity in chromoblastomycosis (1992)
    Springer
    Mycopathologia 119 (1992), S. 73-76 
    Details
    ISSN: 1573-0832
    Keywords: Chromoblastomycosis ; immunity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The cellular immune response of 8 patients from the Brazilian Amazon region with chromoblastomycosis was analyzed. Primary immunological responses of patients were tested by contact sensitization to 2,4-dinitro-chlorobenzene (DNCB), or rejection of first set skin allografts. 2 of 8 patients were reactive to DNCB after sensitization, and skin allograft rejection occured in an average of 14 days. Capacity of patients to mount recall immunological responses was measured by skin testing with two fungal antigens and three bacterial antigens. Delayed skin reaction to trichophytin and Candida antigens was negative in the majority of the patients. However, reactivity to mycobacterial (tuberculin), and bacterial (staphylococcal, streptococcal) antigen was high, or only slightly diminished respectively. The data suggest that patients with chromoblastomycosis have suppressed nonspecific, cell mediated immunity for some antigens (skin allografts, DNCB, fungal antigens), while reactivity to bacterial and mycobacterial antigens is not impaired.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00443936
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