Abstract
Mutants of Escherichia coli were isolated in which transcription of the structural genes for hydrogenase (hyd) and for one of the components of formate dehydrogenase (fdh) (of the formate hydrogen-lyase complex) is coupled with that of the lacZ gene. They were — together with lac fusions of the nifH and nifL genes from Klebsiella — used to study regulation by redox control, of the expression of the respective structural genes. The following results were obtained: (i) β-galactosidase synthesis was fully repressed in the presence of O2 or nitrate (anaerobically), and induced in the absence of an external electron acceptor. Fumarate as terminal electron acceptor only marginally affected nif expression and partially repressed hyd and fdh expression. Redox control of the synthesis of hydrogenase and formate dehydrogenase, therefore, (as well as that of nif) acts at the level of transcription; the size of the redox potential seems to be correlated with the amount of repression; (ii) β-galactosidase synthesis in the hyd:: lac and fdh::lac fusion strains is induced by formate. At high concentrations formate reverses the repression by nitrate and fumarate but not that by oxygen.
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References
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K12 Edition 6. Microbiol Rev 44:1–56
Balch WE, Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethane sulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl Env Microbiol 32:781–791
Barrett EL, Jackson CE, Fukumoto HT, Chang GW (1979) Formate dehydrogenase mutants of Salmonella typhimurium: A new medium for their isolation and new mutant classes. Mol Gen Genet 177:95–101
Begg YA, Whyte JN, Haddock BA (1977) The identification of mutants of Escherichia coli deficient in formate dehydrogenase and nitrate reductase activities using dye indicator plates. FEMS Microbiol Lett 2:47–50
Bukhari AI (1975) Reversal of mutator phage Mu integration. J Mol Biol 96:87–99
Casadaban MJ, Cohen SN (1979) Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76:4530–4533
Chippaux M, Guidici D, Abou-Jaoude A, Casse F, Pascal MC (1978) A mutation leading to total lack of nitrate reductase activity in Escherichia coli K12. Mol Gen Genet 160:225–229
Chippaux M, Bonnefoy-Orth V, Ratouchniak J, Pascal M (1981) Operon fusions in the nitrate reductase operon and study of the control of gene nirR in Escherichia coli. Mol Gen Genet 182:477–479
Cole JA, Wimpenny JWT (1966) The interrelationships of low redox potential cytochrome c552 and hydrogenase in facultative anaerobes. Biochim Biophys Acta 128:419–425
Cox JC, Edwards ES, DeMoss JA (1981) Resolution of distinct seleniumcontaining formate dehydrogenase from Escherichia coli. J Bacteriol 145:1317–1324
Dixon R, Cannon FC, Kondorosi A (1976) Construction of a R plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature 260:268–271
Dobrogosz WJ (1966) Altered endproduct patterns and catabolite repression in E. coli. J Bacteriol 91:2263–2269
Eady RR, Issack R, Kennedy C, Postgate JR, Ratcliffe HD (1978) Nitrogenase synthesis in Klebsiella pneumoniae: comparison of ammonium and oxygen regulation. J Gen Microbiol 104:277–285
Fraenkel DG, Neidhardt FC (1961) Use of chloramphenicol to study control of RNA synthesis in bacteria. Biochim Biophys Acta 53:96–100
Giordano G, Medani C-L, Mandrand-Berthelot M-A, Boxer DH (1983) Formate dehydrogenase from Escherichia coli. FEMS Microbiol Lett 17:171–177
Glick BR, Zeisler J, Banaszuk AM, Friesen JD, Martin WG (1981) The identification and partial characterization of a plasmid containing the gene for the membrane-associated hydrogenase from E. coli. Gene 15:201–206
Graham A, Boxer DH, Haddock BA, Mandrand-Berthelot M-A, Jones RW (1980a) Immunochemical analysis of the membrane-bound hydrogenase of E. coli. FEBS Lett 113:167–172
Graham A, Jenkins HE, Smith NH, Mandrand-Berthelot M-A, Haddock BA, Boxer DH (1980b) The synthesis of formate dehydrogenase and nitrate reductase proteins in various fdh and chl mutants of Escherichia coli. FEMS Microbiol Lett 7:145–151
Haddock BA, Jones CW (1977) Bacterial respiration. Bacteriol Rev 41:47–99
Hom SSM, Hennecke H, Shanmugam KT (1980) Regulation of nitrogenase synthesis in Klebsiella pneumoniae: effect of nitrate. J Gen Microbiol 117:169–179
Knappe J, Schmitt T (1976) A novel reaction of S-adenosyl-l-methionine correlated with the activation of pyruvate formate-lyase. Biochem Biophys Res Commun 71:1110–1117
Lambden PR, Guest JR (1976) Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor. J Gen Microbiol 97:145–160
Lester RL, DeMoss JA (1971) Effects of molybdate and selenite on formate and nitrate metabolism in Escherichia coli. J Bacteriol 105:1006–1014
Lin ECC (1976) Glycerol dissimilation and its regulation in bacteria. Ann Rev Microbiol 30:535–578
Lorowitz W, Clark D (1982) Escherichia coli mutants with a temperaturesensitive alcohol dehydrogenase. J Bacteriol 152:935–938
Lowry OH, Rosebrough NJ, Farr AL, Randall (1951) Protein determination with the Folin phenol reagent. J Biol Chem 193:265–275
Mandrand-Berthelot M-A, Wee MYK, Haddock B (1978) An improved method for identification and characterization of mutants of Escherichia coli deficient in formate dehydrogenase activity. FEMS Microbiol Lett 4:37–40
Merrick M, Hill S, Hennecke H, Hahn M, Dixon R, Kennedy C (1982) Repressor properties of the nifL gene product in Klebsiella pneumoniae. Mol Gen Genet 185:75–81
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Pecher A, Blaschkowski HP, Knappe K, Böck A (1982) Expression of pyruvate formate lyase from the cloned structural gene. Arch Microbiol 132:365–371
Peck HD Jr, Gest H (1957) Formic dehydrogenase and the hydrogenlyase enzyme complex in the coli-aerogenes group. J Bacteriol 73:706–721
Shaw DJ, Guest JR (1981) Molecular cloning of the fnr gene of Escherichia coli K12. Mol Gen Genet 181:95–100
Shaw DJ, Guest JR (1982) Amplification and product identification of the fnr gene of Escherichia coli. J Gen Microbiol 128:2221–2228
St. John RT, Shah VK, Brill WJ (1974) Regulation of nitrogenase synthesis by oxygen in Klebsiella pneumoniae. J Bacteriol 119:266–269
Takagi M, Ishimoto M (1983) Escherichia coli mutants defective in trimethylamine-N-oxide reductase. FEMS Microbiol Lett 17:247–250
Wimpenny JWT, Cole JA (1967) The regulation of metabolism in facultative bacteria. III. The effect of nitrate. Biochim Biophys Acta 148:233–243
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Pecher, A., Zinoni, F., Jatisatienr, C. et al. On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae. Arch. Microbiol. 136, 131–136 (1983). https://doi.org/10.1007/BF00404787
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DOI: https://doi.org/10.1007/BF00404787