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Electron transport phosphorylation driven by glyoxylate respiration with hydrogen as electron donor in membrane vesicles of a glyoxylate-fermenting bacterium (1995)

Friedrich, M. ; Schink, Bernhard

Springer

Archives of microbiology 163 (1995), S. 268-275

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ISSN: 1432-072X

Keywords: Key words Electron transport phosphorylation ; Respiration ; Membrane vesicles ; Anaerobic degradation ; Glyoxylate ; Glycolate ; Hydrogen ; Syntrophy

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The syntrophically glycolate-fermenting bacterium in the methanogenic binary coculture FlGlyM was isolated in pure culture (strain FlGlyR) with glyoxylate as sole substrate. This strain disproportionated 12 glyoxylate to 7 glycolate, 10 CO2, and 3 hydrogen. Glyoxylate was oxidized via the malyl-CoA pathway. All enzymes of this pathway, i.e. malyl-CoA lyase/malate: CoA ligase, malic enzyme, and pyruvate synthase, were demonstrated in cell-free extracts. Glycolate dehydrogenase, hydrogenase, and ATPase, as well as menaquinones as potential electron carriers, were present in the membranes. Everted membrane vesicles catalyzed hydrogen-dependent glyoxylate reduction to glycolate [86–207 nmol min–1 (mg protein)–1] coupled to ATP synthesis from ADP and Pi [38–82 nmol min–1 (mg protein)–1)]. ATP synthesis was abolished entirely by protonophores or ATPase inhibitors (up to 98 and 94% inhibition, respectively) indicating the involvement of proton-motive force in an electron transport phosphorylation driven by a new glyoxylate respiration with hydrogen as electron donor. Measured reaction rates in vesicle preparations revealed a stoichiometry of ATP formation of 0.2–0.5 ATP per glyoxylate reduced.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00393379

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Untersuchungen an Oximkomplexen. III. Nickelchelate des Bis-(diacetylmonoxim-imino)-propans-1,3 und des Bis-(diacetylmonoxim-imino)-äthans-1,2 (1966)

Uhlig, E. ; Friedrich, M.

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 343 (1966), S. 299-307

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ISSN: 0044-2313

Keywords: Chemistry ; Inorganic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: The two compounds mentioned in the title (“[DOH]2pn” and “[DOH]2en”, resp.) react with nickel salts forming the complex compounds Ni{(DOH)(DO)pn}X with X = ClO4, Br, J, SCN; and Ni{(DOH)(DO)en}Y with Y = ClO4, J, SCN. The two perchlorates are supposed to have a planarquadratic structure with ionically bound anions. The other anions, however, interact co-ordinatively with the complex cations, forming thus compounds with, probably, tetragonal-pyramidal configurations.

Notes: Die in der Überschrift genannten Verbindungen (Abkürzung [DOH]2pn und [DOH]2en) bilden mit Nickelsalzen einwertiger Anionen Komplexe der allgemeinen Formeln Ni{(DOH)(DO)pn}X (X = ClO4, Br, J, SCN) und Ni{(DOH)(DO)en}Y (Y = ClO4, J, SCN). Für die beiden Perchlorate wird auf Grund physikalischer Untersuchungen eine planarquadratische Struktur mit ionischer Funktion des Anions vorgeschlagen. Bromid, Jodid und Rhodanid treten dagegen mit den komplexen Kationen [Ni{(DOH)(DO)pn}]+ bzw. [Ni{(DOH)(DO)en}]+ in koordinative Wechselwirkung. Dabei entstehen wahrscheinlich Verbindungen mit tetragonal-pyramidaler Struktur.

Additional Material: 1 Ill.