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  • Plastid transformation  (2)
  • Aminoglycoside-3″-adenyltransferase  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Aminoglycoside-3″-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum (1990)
    Springer
    Plant molecular biology 14 (1990), S. 197-205 
    Details
    ISSN: 1573-5028
    Keywords: Aminoglycoside-3″-adenyltransferase ; chimeric gene ; Nicotiana tabacum ; transgenic plant ; spectinomycin resistance ; streptomycin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The bacterial gene aad A encodes the enzyme aminoglycoside-3″-adenyltransferase that confers resistance to spectinomycin and streptomycin in Escherichia coli. Chimeric genes have been constructed for expression in plants, and were introduced into Nicotiana tabacum by Agrobacterium binary transformation vectors. Spectinomycin or streptomycin in selective concentrations prevent greening of N. tabacum calli. Transgenic clones, however, formed green calli on selective media containing spectinomycin, streptomycin, or both drugs. Resistance was inherited as a dominant Mendelian trait in the seed progeny. Resistance conferred by the chimeric aad A gene can be used as a color marker similar to the resistance conferred by the streptomycin phosphotransferase gene to streptomycin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018560
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  • 2
    Electronic Resource
    Electronic Resource
    Plastid transformation in Arabidopsis thaliana (1998)
    Springer
    Plant cell reports 18 (1998), S. 20-24 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsaadA gene ; Arabidopsis thaliana ; Biolistic DNA delivery ; Plastid transformation ; Spectinomycin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plastid transformation is reported in Arabidopsis thaliana following biolistic delivery of transforming DNA into leaf cells. Transforming plasmid pGS31A carries a spectinomycin resistance (aadA) gene flanked by plastid DNA sequences to target its insertion between trnV and the rps12/7 operon. Integration of aadA by two homologous recombination events via the flanking ptDNA sequences and selective amplification of the transplastomes on spectinomycin medium yielded resistant cell lines and regenerated plants in which the plastid genome copies have been uniformly altered. The efficiency of plastid transformation was low: 2 in 201 bombarded leaf samples. None of the 98 plants regenerated from the two lines were fertile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050525
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  • 3
    Electronic Resource
    Electronic Resource
    In vivo testing of a tobacco plastid DNA segment for guide RNA function in psbL editing (1995)
    Springer
    Molecular genetics and genomics 247 (1995), S. 439-443 
    Details
    ISSN: 1617-4623
    Keywords: RNA editing ; Trans-acting factor ; Guide RNA ; psbL gene ; Plastid transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A C-to-U RNA editing event creates a functional initiation codon for translation of the psbL mRNA in tobacco plastids. Small trans-acting guide RNAs (gRNAs) have been shown to be involved in editing site selection in kinetoplastid mitochondria. A computer search of the tobacco plastid genome (ptDNA) identified such a putative gRNA, a 14-nucleotide sequence motif that is complementary to the psbL mRNA, including the A nucleotide required to direct the C-to-U change. The critical A nucleotide of the putative gRNA gene was changed to G by plastid transformation. We report here that the introduced mutation did not abolish psbL editing. Since no other region of the plastid genome contains significant complementarity to the psbL editing site we suggest that, if gRNAs serve as trans-acting factors for plastid psbL mRNA editing, they either have only a limited complementarity to the editing site, or are encoded in the nuclear genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293145
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