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  • Glucose repression  (2)
  • Life and Medical Sciences  (2)
  • Agassiz Trawl; AGT; ANT-XXIV/2; BC; Box corer; Clione limacina antarctica; Clio pyramidata sulcata; Conorbela antarctica; Conorbela sp.; Date/Time of event; EBS; Echinospira, larvae; Epibenthic sledge; Event label; Gastropoda; Giant box corer; GKG; Iothia coppingeri; Latitude of event; Limacina helicina antarctica; Lissotesta strebeli; Longitude of event; Melanella sp.; Method/Device of event; Oenopota striatula; Onoba subantarctica wilkesiana; Polarstern; PS71/039-1; PS71/039-11; PS71/039-13; PS71/039-15; PS71/039-16; PS71/039-17; PS71 ANDEEP-SYSTCO SCACE; Rectangular midwater trawl; RMT; Scaphander sp.; South Atlantic Ocean; Spongiobranchaea australis; SUIT; Surface and under ice trawl; Toledonia striata
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  • 1
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Fructose-1,6-bisphosphatase ; Glucose repression ; Gene activation ; Gluconeogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis and the FBP1 gene is not transcribed during growth with glucose. Genetic analysis indicated a positive regulation of FBP1 expression after exhaustion of glucose. By linker-deletion analysis, two upstream activation sites (UAS1 and UAS2) were localized and the respective UAS-binding factors (DAP I and DAP II for derepression activating protein) were identified by gel retardation. UAS1 and UAS2 span about 30 bp each, and are separated by approximately 30 bp. Both UAS sites act synergistically. Although UAS1 showed some similarities to the DNA-binding consensus for the general yeast activator Rap1, competition experiments and DEAE-chromatography proved that DAP I and Rap1 correspond to different proteins. Gel retardation by DAP I depended on carbon sources and did not occur in cells growing logarithmically with glucose, whereas a strong retardation signal was obtained with ethanol-grown cells. The present results suggest that DAP I and DAP II are the final regulatory elements for glucose derepression.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Glucose repression ; Glucose derepression ; Regulatory genes ; Expression analysis ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Yeast strains carrying one of the two regulatory mutations cat1 and cat3 are defectve in derepression of several glucose-repressible enzymes that are necessary for utilizing non-fermentable carbon sources. Hence, these strains fail to grow on ethanol, glycerol or acetate. The synthesis of isocitrate lyase, malate synthase, malate dehydrogenase and fructose-1,6-bisphosphatase is strongly affected in cat1 and cat3 strains. Genes CAT1 and CAT3 have been isolated by complementation of the cognate, mutations after transformation with an episomal plasmid gene library. The restriction map of CAT1 proved its allelism to the earlier isolated SNF1 gene. Both genes appear to exist as single-copy genes per haploid genome as indicated by Southern hybridization. Northern analysis has shown that the 1.35 kb CAT3 mRNA is constitutively expressed, independent of the carbon source in the medium. Derepression studies with CAT3 transformants using a multi-copy plasmid showed over-expression of glyoxylate cycle enzymes. This result would be consistent with a direct effector function for the CAT3 gene product.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 1041-1050 
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; STRE ; stress response ; genomics ; bioinformatics ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Stress response elements (STREs, core consensus AG4 or C4T) have been demonstrated previously to occur in the upstream region of a number of genes responsive to induction by a variety of stress signals. This stress response is mediated by the homologous transcription factors Msn2p and Msn4p, which bind specifically to STREs. Double mutants (msn2 msn4) deficient in these transcription factors have been shown to be hypersensitive to severe stress conditions. To obtain a more representative overview of the set of yeast genes controlled via this regulon, a computer search of the Saccharomyces cerevisiae genome was carried out for genes, which, similar to most known STRE-controlled genes, exhibit at least two STREs in their upstream region. In addition to the great majority of genes previously known to be controlled via STREs, 69 open reading-frames were detected. Expression patterns of a set of these were examined by grid filter hybridization, and 14 genes were examined by Northern analysis. Comparison of the expression patterns of these genes demonstrates that they are all STRE-controlled although their detailed expression patterns differ considerably. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 2 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    BioEssays 17 (1995), S. 959-965 
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In the yeast Saccharomyces cerevisiae three positive transcriptional control elements are activated by stress conditions: heat shock elements (HSEs), stress response elements (STREs) and AP-1 responsive elements (AREs). HSEs bind heat shock transcription factor (HSF), which is activated by stress conditions causing accumulation of abnormal proteins. STREs mediate transcriptional activation by multiple stress conditions. They are controlled by high osmolarity via the HOG signal pathway, which comprises a MAP kinase module and a two-component system homologous to prokaryotic signal transducers. AREs bind the transcription factor Yap1p. The three types of control elements seem to have overlapping, but distinct functions. Some stress proteins encoded by HSE-regulated genes are necessary for growth of yeast under moderate stress, products of STRE-activated genes appear to be important for survival under severe stress and ARE-controlled genes may mainly function during oxidative stress and in the response to toxic conditions, such as caused by heavy metal ions.
    Additional Material: 4 Ill.
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  • 5
    Publication Date: 2024-06-25
    Keywords: Agassiz Trawl; AGT; ANT-XXIV/2; BC; Box corer; Clione limacina antarctica; Clio pyramidata sulcata; Conorbela antarctica; Conorbela sp.; Date/Time of event; EBS; Echinospira, larvae; Epibenthic sledge; Event label; Gastropoda; Giant box corer; GKG; Iothia coppingeri; Latitude of event; Limacina helicina antarctica; Lissotesta strebeli; Longitude of event; Melanella sp.; Method/Device of event; Oenopota striatula; Onoba subantarctica wilkesiana; Polarstern; PS71/039-1; PS71/039-11; PS71/039-13; PS71/039-15; PS71/039-16; PS71/039-17; PS71 ANDEEP-SYSTCO SCACE; Rectangular midwater trawl; RMT; Scaphander sp.; South Atlantic Ocean; Spongiobranchaea australis; SUIT; Surface and under ice trawl; Toledonia striata
    Type: Dataset
    Format: text/tab-separated-values, 90 data points
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