Publication Date:
2021-05-19
Description:
At present, the aquaculture industry to provide proper instructions in the field of health management, including
production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and
identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms
have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the
accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance
of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens,
diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and
emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated
pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling
of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them
had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified
are:
Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014
(GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012
(GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017
(GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019
(GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not
detected.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Bacteria
;
Fungi
;
Ribotyping
;
16S rDNA
;
Shrimp
;
Specific pathogen free
;
Vibrio rotiferianus
;
Vibrio owensii
;
Vibrio brasiliensis
;
Vibrio azureus
;
Agarivorans gilvus
;
Vibrio alginolyticus
;
GenBank
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
64pp.
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