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  • 1
    Electronic Resource
    Electronic Resource
    Accurate Mass Measurements Using MALDI-TOF with Delayed Extraction (1997)
    Springer
    The protein journal 16 (1997), S. 363-369 
    Details
    ISSN: 1573-4943
    Keywords: Accurate mass ; MALDI-TOF ; delayed extraction ; mass spectrometry ; peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is now an essential tool in biopolymer analysis. Sensitivity and mass range are unsurpassed, but mass measurement accuracy and resolution have been limited. With delayed extraction and a reflecting analyzer, mass measurements using MALDI-TOF can be made with an accuracy of a few parts per million (ppm). It is possible to distinguish Lys from Gln in peptides, and to determine the elemental composition of smaller molecules (mass 100–500). In database searching strategies, a smaller mass window, resulting from an increase in mass accuracy, greatly decreases the number of possible candidates. Mass measurement accuracy with errors less than 5 ppm is demonstrated on a mixture of 12 peptides ranging in mass from ca. 900 to 3700 Da. Mass measurements on 13 peaks in an unseparated tryptic digest of myoglobin gave results with an overall average error less than 3.5 ppm, with a maximum error of 7 ppm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026376403468
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  • 2
    Electronic Resource
    Electronic Resource
    Variation in some agronomically important characters in a germplasm collection of beet (Beta vulgaris L.) (1985)
    Springer
    Euphytica 34 (1985), S. 449-455 
    Details
    ISSN: 1573-5060
    Keywords: Beta vulgaris ; Beta vulgaris ssp. maritima ; Peronospora farinosa betae ; downy mildew ; beet virus yellows ; Myzus persicae ; aphid resistance ; cytoplasmic male sterility ; maintainer genes ; germplasm ; genetic resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A geographically representative selection of germplasm of Beta vulgaris, section Beta has been assessed for characteristics important in sugarbeet breeding, including downy mildew resistance, resistance to aphid colonisation and infection by the beet virus yellow complex. The occurrence of maintainer lines for cytoplasmic male-steriles was also investigated. Desirable qualities were found in some accessions, including nothern European wild vulgaris ssp. maritima and some old multigerm cultivars of fodder beets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022941
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    Paper (German National Licenses)
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