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  • Glucose repression  (2)
  • AWI; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; SPP1158
  • 1
    ISSN: 1432-0983
    Schlagwort(e): Saccharomyces cerevisiae ; Fructose-1,6-bisphosphatase ; Glucose repression ; Gene activation ; Gluconeogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Fructose-1,6-bisphosphatase is a key enzyme in gluconeogenesis and the FBP1 gene is not transcribed during growth with glucose. Genetic analysis indicated a positive regulation of FBP1 expression after exhaustion of glucose. By linker-deletion analysis, two upstream activation sites (UAS1 and UAS2) were localized and the respective UAS-binding factors (DAP I and DAP II for derepression activating protein) were identified by gel retardation. UAS1 and UAS2 span about 30 bp each, and are separated by approximately 30 bp. Both UAS sites act synergistically. Although UAS1 showed some similarities to the DNA-binding consensus for the general yeast activator Rap1, competition experiments and DEAE-chromatography proved that DAP I and Rap1 correspond to different proteins. Gel retardation by DAP I depended on carbon sources and did not occur in cells growing logarithmically with glucose, whereas a strong retardation signal was obtained with ethanol-grown cells. The present results suggest that DAP I and DAP II are the final regulatory elements for glucose derepression.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 209 (1987), S. 366-373 
    ISSN: 1617-4623
    Schlagwort(e): Glucose repression ; Glucose derepression ; Regulatory genes ; Expression analysis ; Saccharomyces cerevisiae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Yeast strains carrying one of the two regulatory mutations cat1 and cat3 are defectve in derepression of several glucose-repressible enzymes that are necessary for utilizing non-fermentable carbon sources. Hence, these strains fail to grow on ethanol, glycerol or acetate. The synthesis of isocitrate lyase, malate synthase, malate dehydrogenase and fructose-1,6-bisphosphatase is strongly affected in cat1 and cat3 strains. Genes CAT1 and CAT3 have been isolated by complementation of the cognate, mutations after transformation with an episomal plasmid gene library. The restriction map of CAT1 proved its allelism to the earlier isolated SNF1 gene. Both genes appear to exist as single-copy genes per haploid genome as indicated by Southern hybridization. Northern analysis has shown that the 1.35 kb CAT3 mRNA is constitutively expressed, independent of the carbon source in the medium. Derepression studies with CAT3 transformants using a multi-copy plasmid showed over-expression of glyoxylate cycle enzymes. This result would be consistent with a direct effector function for the CAT3 gene product.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    facet.materialart.
    Unbekannt
    PANGAEA
    In:  Supplement to: Brandt, Angelika; Bathmann, Ulrich; Brix, Saskia; Cisewski, Boris; Flores, Hauke; Göcke, C; Janussen, Dorte; Krägefsky, Sören; Kruse, Svenja; Leach, Harry; Linse, Katrin; Pakhomov, Evgeny A; Peeken, Ilka; Riehl, Torben; Sauter, Eberhard-Jürgen; Sachs, Oliver; Schüller, M; Schrödl, M; Schwabe, E; Strass, Volker H; van Franeker, Jan Andries; Wilmsen, Markus (2011): Maud Rise - a snapshot through the water column. Deep Sea Research Part II: Topical Studies in Oceanography, 58(19-20), 1962-1982, https://doi.org/10.1016/j.dsr2.2011.01.008
    Publikationsdatum: 2024-03-23
    Beschreibung: The benthic fauna was investigated during the expedition ANT-XXIV/2 (2007/08) in relation to oceanographic features, biogeochemical properties and sediment characteristics, as well as the benthic, pelagic and air-breathing fauna. The results document that Maud Rise (MR) differs distinctly from surrounding deep-sea basins investigated during previous Southern Ocean expeditions (ANDEEP 2002, 2005). Considering all taxa, the overall similarity between MR and adjacent stations was low (~20% Bray-Curtis-Similarity), and analyses of single taxa show obvious differences in species composition, abundances and densities. The composition and diversity of bivalves of MR are characterised by extremely high abundances of three species, especially the small sized Vesicomya spp. Exceptionally high gastropod abundance at MR is due to the single species Onoba subantarctica wilkesiana, a small brooder that may prey upon abundant benthic foraminiferas. The abundance and diversity of isopods also show that one family, Haplomunnidae, occurs with a surprisingly high number of individuals at MR while this family was not found at any of the 40 bathyal and abyssal ANDEEP stations. Similarly, polychaetes, especially the tube-dwelling, suspension-feeder fraction, are represented by species not found at the comparison stations. Sponges comprise almost exclusively small specimens in relatively high numbers, especially a few species of Polymastiidae. Water-column sampling from the surface to the seafloor, including observations of top predators, indicate the existence of a prospering pelagic food web. Local concentrations of top predators and zooplankton are associated with a rich ice-edge bloom located over the northern slope of MR. There the sea ice melts, which is probably accelerated by the advection of warm water at intermediate depth. Over the southern slope, high concentrations of Antarctic krill (Euphausia superba) occur under dense sea ice and attract Antarctic Minke Whales (Balaenoptera bonaerensis) and several seabird species. These findings suggest that biological prosperity over MR is related to both oceanographic and sea-ice processes. Downward transport of the organic matter produced in the pelagic realm may be more constant than elsewhere due to low lateral drift over MR.
    Schlagwort(e): AWI; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; SPP1158
    Materialart: Dataset
    Format: application/zip, 7 datasets
    Standort Signatur Erwartet Verfügbarkeit
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