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  • A. pseudogonyaulax isolated from Limfjord; Absorption cross section of PSII photochemistry; Alexandrium pseudogonyaulax; Alexandrium pseudogonyaulax, cell size; Alexandrium pseudogonyaulax, strain; Alexandrium sp.; beta-Carotene per cell; bottom-up; Carbon, organic, particulate, per cell; Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS; Chlorophyll a per cell; Chlorophyll c1+c2 per cell; Comment; Connectivity between photosystems PSI and PSII; Date/time end, experiment; Date/time start, experiment; Diadinoxanthin per cell; Diatoxanthin per cell; Dinoxanthin per cell; Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX; Fluorescence, maximum; Fluorescence, variable; Functional photosystem II reaction centers; Goniodomin A; Goniodomin A per cell; growth; High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters; Intraspecific variability; Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000; Laboratory experiment; Light sensor (ULM-500, Walz GmbH, Effeltrich, Germany); Maximal electron transport rate, relative; MULT; Multiple investigations; Nitrogen; Nitrogen, organic, particulate, per cell; Peridinin per cell; photophysiology; phycotoxins; Probability of an RCII being closed during the first flashlet of an single turnover saturation phase; Quantum yield efficiency of photosystem II; Radiation, photosynthetically active; Replicate; T-AP-N; Thyborøn, Limfjord, Denmark; Time, incubation; Time constant for the initial part of an single turnover relaxation phase; Treatment: light intensity; Type of study; Violaxanthin per cell; Visual identification (VID) of Lugol preserved samples; Zeaxanthin per cell  (1)
  • A. pseudogonyaulax isolated from Limfjord; Alexandrium pseudogonyaulax; Alexandrium pseudogonyaulax, cell size; Alexandrium pseudogonyaulax, strain; Alexandrium sp.; bottom-up; Carbon, organic, particulate; Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS; Comment; Date/time end, experiment; Date/time start, experiment; Goniodomin A; growth; Intraspecific variability; Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000; Laboratory experiment; MULT; Multiple investigations; Nitrogen; Nitrogen, organic, particulate; photophysiology; phycotoxins; Replicate; T-AP-N; Thyborøn, Limfjord, Denmark; Time, incubation; Treatment: nitrogen; Type of study; Visual identification (VID) of Lugol preserved samples  (1)
  • AWI_BioOce; Biological Oceanography @ AWI  (1)
  • Activity description; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aurelia aurita; Behaviour; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon content per individual; Carbon dioxide; Cnidaria; Coast and continental shelf; Dry mass per individual; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Identification; Laboratory experiment; Mortality; Mortality/Survival; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Oxygen; Oxygen saturation; Oxygen saturation, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Registration number of species; Salinity; Sample code/label; Single species; Species; Temperate; Temperature; Temperature, water; Treatment; Type; Uniform resource locator/link to reference; Zooplankton  (1)
  • Alexandrium sp.; bottom-up; growth; Laboratory experiment; Light; Nitrogen; photophysiology; phycotoxins
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  • 1
    Publikationsdatum: 2023-07-06
    Beschreibung: The copepod Temora longicornis depends on constant prey availability. Given that climate change may induce food regime shifts, our research goal was to understand copepod energy allocation in relation to diet quality. The working hypothesis was that Temora performs better on the diet whose elemental ratio is closest to its own. Diatoms (Diat) and dinoflagellates (Dino) cultured in nutrient-replete (+) and nitrogen-depleted (-) conditions were fed to the copepods. Ingestion, respiration, excretion, and egg and faecal pellet production rates were measured. Carbon (C) and nitrogen (N) budgets were built to investigate differences in dietary C and N partitioning. Copepods fed with nitrogen-depleted diatoms (Diat-), which had the most different C:N ratio to that of T. longicornis, had high metabolic losses and low growth. In contrast, copepods fed with nitrogen-rich dinoflagellates (Dino+) of closer C:N ratio had high metabolic losses, but the highest investment into somatic and reproductive tissue. The results indicate that, under the current climate scenario and nutrient-replete conditions, dinoflagellates are a better food source for T. longicornis. Furthermore, ingestion, respiration, and faecal pellet integrity are adversely affected by low quality food; and egestion is a main pathway in copepods for eliminating excess carbon.
    Schlagwort(e): AWI_BioOce; Biological Oceanography @ AWI
    Materialart: Dataset
    Format: application/zip, 9 datasets
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2024-03-15
    Beschreibung: Global change is affecting marine ecosystems through a combination of different stressors such as warming, ocean acidification and oxygen depletion. Very little is known about the interactions among these factors, especially with respect to gelatinous zooplankton. Therefore, in this study we investigated the direct effects of pH, temperature and oxygen availability on the moon jellyfish Aurelia aurita, concentrating on the ephyral life stage. Starved one-day-old ephyrae were exposed to a range of pCO2 (400–4000 ppm) and three different dissolved oxygen levels (from saturated to hypoxic conditions), in two different temperatures (5 and 15 °C) for 7 days. Carbon content and swimming activity were analysed at the end of the incubation period, and mortality noted. General linearized models were fitted through the data, with the best fitting models including two- and three-way interactions between pCO2, temperature and oxygen concentration. The combined effect of the stressors was small but significant, with the clearest negative effect on growth caused by the combination of all three stressors present (high temperature, high CO2, low oxygen). We conclude that A. aurita ephyrae are robust and that they are not likely to suffer from these environmental stressors in a near future.
    Schlagwort(e): Activity description; Alkalinity, total; Alkalinity, total, standard deviation; Animalia; Aragonite saturation state; Aurelia aurita; Behaviour; Bicarbonate ion; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon content per individual; Carbon dioxide; Cnidaria; Coast and continental shelf; Dry mass per individual; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Identification; Laboratory experiment; Mortality; Mortality/Survival; North Atlantic; OA-ICC; Ocean Acidification International Coordination Centre; Oxygen; Oxygen saturation; Oxygen saturation, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; pH, standard deviation; Registration number of species; Salinity; Sample code/label; Single species; Species; Temperate; Temperature; Temperature, water; Treatment; Type; Uniform resource locator/link to reference; Zooplankton
    Materialart: Dataset
    Format: text/tab-separated-values, 6723 data points
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 3
    Publikationsdatum: 2024-06-06
    Beschreibung: The effect of different nitrogen sources (N-deplete, NO3-, NH4+, Urea) on the growth response and toxin content of Alexandrium pseudogonyaulax, isolated in 2020 from the Danish Limfjord, were investigated by means of a bottle incubation experiment. The experiment was started on the 18th October 2021 and finished on the 13th December 2021. This dataset contains all data collected within the experiment, including cell counts, cell sizes, goniodomin A toxin contents, particular organic carbon.
    Schlagwort(e): A. pseudogonyaulax isolated from Limfjord; Alexandrium pseudogonyaulax; Alexandrium pseudogonyaulax, cell size; Alexandrium pseudogonyaulax, strain; Alexandrium sp.; bottom-up; Carbon, organic, particulate; Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS; Comment; Date/time end, experiment; Date/time start, experiment; Goniodomin A; growth; Intraspecific variability; Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000; Laboratory experiment; MULT; Multiple investigations; Nitrogen; Nitrogen, organic, particulate; photophysiology; phycotoxins; Replicate; T-AP-N; Thyborøn, Limfjord, Denmark; Time, incubation; Treatment: nitrogen; Type of study; Visual identification (VID) of Lugol preserved samples
    Materialart: Dataset
    Format: text/tab-separated-values, 2097 data points
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 4
    Publikationsdatum: 2024-06-06
    Beschreibung: The effect of different nitrogen sources (Experiment 1: N-deplete, NO3-, NH4+, Urea) and different light intensities (Experiment 2: 20, 100 and 200 μmol photons m-2 s-1) on the growth response and toxin content of Alexandrium pseudogonyaulax, isolated in 2020 from the Danish Limfjord, were investigated by means of bottle incubation experiments. In the 2nd experiment, the pigment content and photophysiological response of A. pseudogonyaulax was additionally assessed. The 1st experiment was started on the 18th October 2021 and finished on the 13th December 2021, while the 2nd experiment was started on the 16th March 2022 and finished on the 14th April 2022. This dataset contains all data collected within the two experiments, including cell counts, cell sizes, goniodomin A toxin contents, particular organic carbon and nitrogen, pigment contents (only exp. 2) and photophysiological parameters (only exp. 2).
    Schlagwort(e): Alexandrium sp.; bottom-up; growth; Laboratory experiment; Light; Nitrogen; photophysiology; phycotoxins
    Materialart: Dataset
    Format: application/zip, 3 datasets
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2024-06-06
    Beschreibung: The effect of different light intensities (20, 100 and 200 μmol photons m-2 s-1) on the growth response, toxin/pigment/nutrient content and photophysiology of Alexandrium pseudogonyaulax, isolated in 2020 from the Danish Limfjord, were investigated by means of a bottle incubation experiment. The experiment was started on the 16th March 2022 and finished on the 14th April 2022. This dataset contains all data collected within the two experiments, including cell counts, cell sizes, goniodomin A toxin contents, particular organic carbon and nitrogen, pigment contents and photophysiological parameters. Chlorophyll a fluorescence measurements were performed using a Fast Repetition Rate fluorometer (FRRf) coupled to a FastAct Laboratory system (FastOcean PTX, both from Chelsea Technologies Group) in the exponential growth phase. The parameters were determined after 10 min of dark acclimation.
    Schlagwort(e): A. pseudogonyaulax isolated from Limfjord; Absorption cross section of PSII photochemistry; Alexandrium pseudogonyaulax; Alexandrium pseudogonyaulax, cell size; Alexandrium pseudogonyaulax, strain; Alexandrium sp.; beta-Carotene per cell; bottom-up; Carbon, organic, particulate, per cell; Centrifuged off, solubilized in methanol, spin-filtered; measured with ultra-high-performance liquid chromatography (UHPLC); coupled with triple quadrupole mass spectrometer (TQMS), Waters, Xevo TQ-XS; Chlorophyll a per cell; Chlorophyll c1+c2 per cell; Comment; Connectivity between photosystems PSI and PSII; Date/time end, experiment; Date/time start, experiment; Diadinoxanthin per cell; Diatoxanthin per cell; Dinoxanthin per cell; Fast repetition rate fluorometer (FRRf), Chelsea Technologies Group; coupled with FastAct laboratory system, Chelsea Technologies Group, FastOcean PTX; Fluorescence, maximum; Fluorescence, variable; Functional photosystem II reaction centers; Goniodomin A; Goniodomin A per cell; growth; High performance liquid chromatography (HPLC) separation module, Waters, Alliance 2695; coupled with 2996 photodiode array detector, Waters; Intraspecific variability; Kept in dark at -20°C on precombusted Gf/f filter; dried for 24 h at 60 °C, then added conc. HCl and dried for 24 h at 60 °C; measured with Elemental analyser, HEKAtech, Euro EA 3000; Laboratory experiment; Light sensor (ULM-500, Walz GmbH, Effeltrich, Germany); Maximal electron transport rate, relative; MULT; Multiple investigations; Nitrogen; Nitrogen, organic, particulate, per cell; Peridinin per cell; photophysiology; phycotoxins; Probability of an RCII being closed during the first flashlet of an single turnover saturation phase; Quantum yield efficiency of photosystem II; Radiation, photosynthetically active; Replicate; T-AP-N; Thyborøn, Limfjord, Denmark; Time, incubation; Time constant for the initial part of an single turnover relaxation phase; Treatment: light intensity; Type of study; Violaxanthin per cell; Visual identification (VID) of Lugol preserved samples; Zeaxanthin per cell
    Materialart: Dataset
    Format: text/tab-separated-values, 3398 data points
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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