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  • Analytical Chemistry and Spectroscopy  (9)
  • Pisum  (5)
  • 1H-13C COSY NMR
  • 1
    Electronic Resource
    Electronic Resource
    The initiation of legumin synthesis in immature embryos of Pisum sativum L. grown in vivo and in vitro (1980)
    Springer
    Planta 149 (1980), S. 454-460 
    Details
    ISSN: 1432-2048
    Keywords: Embryo culture ; Enzyme-linked immunosorbent assay (ELISA) ; Legumin ; Pisum ; Protein (storage) ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A highly sensitive immunoassay has been used for the detection of a major storage protein, legumin, in embryos of Pisum sativum L.; with this technique nanogram quantities could be measured. In the two varieties tested, legumin could be detected in embryos in vivo, when they had attained a fresh weight of 2·10-3 g and 3·10-3 g, respectively. Contrary to earlier claims, embryos cultured in vitro were shown to be capable of initiating legumin synthesis. This capacity to initiate legumin synthesis was confirmed by two-dimensional isoelectric focusing-electrophoresis and fluorography; embryos harvested before initiation of legumin synthesis and cultured in radioactive medium were shown to have synthesized legumin subunits. The amounts of legumin and total protein synthesized per unit fresh weight were consistently greater in vitro than in equivalent embryos grown in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385747
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  • 2
    Electronic Resource
    Electronic Resource
    Pollen irradiation and the transfer of maternal genes in Pisum sativum (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 245-248 
    Details
    ISSN: 1432-2242
    Keywords: Pollen ; Irradiation ; Gene transfer ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pollen of Pisum sativum was exposed to doses of 900 to 6,000 r of X-rays prior to pollinating a multiply marked genotype. The first generation progeny closely resembled that produced with unirradiated pollen. In the second generation, five loci were monitored, and the results showed that irradiation enhanced the proportion of maternal information transmitted to the progeny; the practical implications of the data, as well as the mechanism underlying the effect are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317046
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  • 3
    Electronic Resource
    Electronic Resource
    Organization and mapping of legumin genes in Pisum (1986)
    Springer
    Molecular genetics and genomics 202 (1986), S. 280-285 
    Details
    ISSN: 1617-4623
    Keywords: Legumin genes ; Genetic loci ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The organization of three classes of legumin gene has been studied by exploiting restriction fragment length polymorphisms. Hybridization of cDNA probes, representing different sub-families of legumin gene, to DNA from F2 and F6 plants from selected crosses showed that there was a tight linkage of individual genes within each of two sub-families. One of these sub-families has been mapped to a locus on chromosome 7 close to r, while the other maps to a locus near a on chromosome 1. The results from one of the crosses analyzed indicated that a third class of legumin gene is also linked to a.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331651
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  • 4
    Electronic Resource
    Electronic Resource
    Vicilin genes ofPisum (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 164-169 
    Details
    ISSN: 1617-4623
    Keywords: Vicilin ; Genetic loci ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe four genomic clones of pea 7S storage protein gene, one of which corresponds to convicilin, and the others to vicilin. Hybridization studies exploiting these clones, and previously identified cDNA clones, have enabled us to define six different loci. Three of these loci have been mapped to positions on chromosome 7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02428047
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  • 5
    Electronic Resource
    Electronic Resource
    The cytology of cotyledon cells and the induction of giant polytene chromosomes inPisum sativum (1979)
    Springer
    Protoplasma 101 (1979), S. 73-80 
    Details
    ISSN: 1615-6102
    Keywords: Cotyledon culture ; Pisum ; Polytene chromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cotyledon cells ofPisum sativum have high DNA contents. By appropriate culture techniques, some of these cells can be triggered into division. Two types of dividing nuclei were seen. Firstly those that were polyploid with metaphases containing chromosome numbers ranging in value from 4 x to 32 x. Included among these were unexpected numbers equivalent to 12 x and 14 x. Secondly there were cells containing giant polytene chromosomes and these progressed from prophase to a metaphase where the polytene chromosomes separated into constituent single chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01293436
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  • 6
    Electronic Resource
    Electronic Resource
    The measurement of clonidine in human plasma and urine by combined gas chromatography mass spectrometry with ammonia chemical ionization (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 8 (1981), S. 500-502 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The dimethyl derivatives of clonidine and [2H4]clonidine give good chemical ionization mass spectra when ammonia is used as reagent gas. A gas chromatographic mass spectrometric selected ion monitoring assay for the estimation of clonidine in plasma and urine using ammonia chemical ionization is described.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200081007
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  • 7
    Electronic Resource
    Electronic Resource
    Letter to the editors (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 7 (1980), S. 183-183 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200070410
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  • 8
    Electronic Resource
    Electronic Resource
    An assay for 2-amino-3,8-dimethylimidazo[4,5-ƒ]-quinoxaline and 2-amino-3,4,8-trimethylimidazo-[4,5-ƒ]quinoxaline in fried beef using capillary column gas chromatography electron capture negative Ion chemical ionization mass spectrometry (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 16 (1988), S. 221-224 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A gas chromatographic/mass spectormetric assay has been developed for the simultaneous measurement of 2-amino-3,8-dimethylimidazo[4,5-ƒ]quinoxaline (MeIQx) and 2-amino-3,4,8-trimethylimidazo[4,5-ƒ]quinoxaline (DiMeIQx) in fried beef. The method employs capillary column gas chromatography, electron capture negative ion chemical ionization mass spectrometry and a stable isotope labelled analogue of MeIQx as common internal standard. Two patties of lean minced beef which had been cooked separately were analysed and found to contain both compounds (patty 1:2.4 ng MeIQx g-1 meat, 1.2 ng DiMeIQx g-1 meat; patty 2: 1.3 ng MeIQx g-1 meat, 0.5 ng DiMeIQx g-1 meat). Neither compound was present in the meat prior to cooking.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200160140
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  • 9
    Electronic Resource
    Electronic Resource
    Synthetic pyrethroid insecticides analysis by gas chromatography/mass spectrometry operated in negative-ion chemical ionization mode in soil, moss and fish tissue (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1411-1417 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: A gas chromatographic/mass spectrometric method was developed for the simultaneous determination of five synthetic pyrethroid insecticides in soil, moss and fish tissue. These pyrethroids were extracted with hexane-dichloromethane by ultrasonication and cleaned up on Florisil (soil and moss) and mixed acid/base alumina (fish) columns prior to determination by gas chromatography/negative-ion chemical ionization mass spectrometry in selected ion monitoring mode. All the pyrethroids were analyzed simultaneously in a single run on a DB5-MS 15 m capillary column. Recoveries of the pyrethroids from the three matrices at fortification levels of 10, 50 and 100 μg/kg ranged from 80 to 117%. Four determinations were made at each concentration level for each matrix. The practical determination limit of the method was in the range 0.5 to 5 μg/kg depending on the compound. This method was also applied to samples obtained from a contaminated ecosystem.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091418
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  • 10
    Electronic Resource
    Electronic Resource
    Bistrifluoromethylaryl derivatives for drug analysis by gas chromatography electron capture negative ion chemical ionization mass spectrometry. Application to the measurement of (N-dicyclopropylmethyl)amino-2-oxazoline in plasma (1985)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 12 (1985), S. 230-237 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A gas chromatographic mass spectrometric assay for (N-dicyclopropylmethyl)amino-2-oxazoline in plasma with a detection limit of 0.1 ng ml-1 was required. Various fluoroaryl derivatives of this compound (code name S3341) were synthesized and their positive ion chemical ionization and electron capture negative ion chemical ionization mass spectra recorded. While fluorobenzyl derivatives of S3341 could be made by heating with the requisite benzyl bromide and diisopropylethylamine in acetonitrile, initial efforts to synthesize corresponding fluorobenzoyl derivatives using a benzoyl chloride in dry ethyl acetate at 60 °C were unsuccessful. Mass spectral data indicated that only a fragment of the oxazoline ring was retained in the reaction product and that an N-(2-chloroethyl)benzamide was formed. However, when diisopropylethylamine was included in the reaction mixture, a benzoyl derivative of the complete molecule was obtained. The mechanisms of these reactions are discussed. The negative ion mass spectrum of the 3,5-bistrifluoromethylbenzoyl derivative of S3341 has a base peak at m/z 420 (the molecular ion) and, when this ion is specifically monitored, an amount of derivative equivalent to 1 pg of S3341 can be detected. This allowed the development of an assay for S3341 in plasma with a precision of 9% (SD) at 0.2 ng ml-1 and a lower limit for quantitative determination of 0.1 ng ml-1.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200120509
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