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  • Khouzestan Province  (3)
  • 16S rRNA  (2)
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  • 1
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    Monitoring of fresh water fish farms in north of khouzestan province for mortality emphasis on silver carp (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25647 | 18721 | 2018-10-08 08:31:58 | 25647 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Carp fishes culture is one of the most important subsector fisheries industry in Khouzestan province. Every year in summer high mortality occurred in carp fishes farms specially in silver carp of north Khouzestan province. Inspection of silver carp for identification microorganisms agents (Bacteria, Fungi, Parasite and virus: SVC,KHV), determine total plate count, examination physic chemical factors of farms water, histopathology, identification planktons, the main objectives of this study, which was held in 2012. 180 silver carp from 5 farms (10 ponds) with high mortality in years ago were collected in north Khouzestan province and then differents parts of fish body including: intestine, gills, body surface, eyes and liver were examined. Physico chemical parameters and plankton sampled monthly. Physico chemical parameters of water were measured all over culture period for 960 times as follows: Do(4.45-8.5 ppm), BOD5(1.1-9.25 ppm), temp(19-33c), pH(6.82-8.6), total hardness(348-10053), turbidity(5-56 FTU), Amonia(0.001- 0.06 ppm) and co2( 0.88-13.2). From a total 180 fish examined, 136 (75%) showed unknown algae infestation and 126 (70%) showed parasitic (Dactylogyrus, Gyrodactylus and metacercaria Diplostomum). The results of biotic parameters in farms showed that cyanophycae (Cylindropermopsis, Merismopedia and Nitzeshia) were the most group of phytoplanktons. Histopathological studies have shown necrosis in tobules kidney, degeneration hepatocyte and hyperplasia in gill cells and also results demonstrated the mean of total plate count (0-376 CFU/ml103). 60 samples with sighn severe hemorrhagic on surface body were collected for virology studies by PCR procedure (IQ2000 kit) and have shown which 3 sample suspected to KHV but SVC was not identified.
    Keywords: Aquaculture ; Iran ; Khouzestan Province ; Silver Carp ; Water physic chemical factor ; Microorganisms ; Histopathology ; Fresh water ; Mortality ; Fisheries ; Bacteria ; Fungi ; Parasite ; Virus ; Planktons ; Dactylogyrus ; Gyrodactylus ; Cyanophycae ; Cylindropermopsis ; Nitzeshia ; PCR
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 110
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  • 2
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    Epidemiological study on some environmental and management parameters affecting on WSD occurrence in Fenneropenaeus indicus and Penaeus vannamei (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25652 | 18721 | 2018-10-08 08:43:54 | 25652 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: For the first time white spot disease (WSD) was reported in shrimp farms of khoozestan province, in southwest of IRAN in 2002. Then in 2005 the neighbor province, boushehr, was contaminated. In 2008 WSD outbreak reported in sistan-bloochestan province in southeast of Iran. In 2015 all of southern shrimp farms of country except Hormozgan, the middle southern province, which has remained free of WSD, are being contaminated. White Spot disease suspended shrimp culture in thousands hectares of shrimp farms. Considering that white spot disease has not been observed in Hormozgan province yet, the question is; to what extent environmental and management factors participated in preventing WSD outbreak or cause WSD outbreak. In this study (20102012), the effects of environmental factors and management, stressors that decrease immune system function of shrimp are discussed. In addition, the role of pathogen as the main factor of outbreak is discussed. The goal of this study is to define environmental parameters and management practices associates with outbreak of white spot disease in affected provinces and discover reasons of being Hormozgan province free of this disease. In this study the role of the local environmental factors and management practice stressors in susceptibility to WSD was determine. Both the effects of environmental factors in water of ponds including total ammonia, nitrogen, dissolved oxygen, pH, salinity, transparency, and temperature and management issues related to biosecurity are studied. There were overlaps on physical and chemical parameter values obtained in clear areas with contaminated areas .Results of the data analysis suggest that lack of association with WSD incidence was 7 times greater than WSD incidence despite of disease outbreak in sistan-bloochestan province, so other sources of white spot disease virus incidence was suspected in affected areas. Histopathological examinations and polymerase chain reaction (PCR) tests during project performance did not reveal white spot disease virus evidences in post larvae examined from khoozestan province stocked in farms but disease outbreak was happened in that farms , so we suspected to management practice include feed , pond preparation and carrier of disease. Recorded values of temperature and salinity in some months during inspection in Hormozgan province specified stressful condition that may lead to WSD outbreak, however the disease did not appear. Therefore the hypothesis that the water physical and chemical conditions are reasons to prevent disease outbreak in Hormozgan province is being rejected. The policy of Hormozgan’s fishery authorities, to replaced Fenneropenaeus indicus with specific pathogen free Litopenaeus vannamei, that is more resistant to some of diseases, before incidence of WSD in farms and to before being endemic in the Hormozgan province, made an advantage compare to affected southern provinces that introduced Litopenaeus vannamei after WSD prevalence to their farms. However it does not guarantee to maintain current trend of being Hormozgan province farms free of white spot disease. Therefore establishing the principals of biosecurity are strongly emphasized. Strategies taken by the proficient authorities in preparation of SPF shrimp broodstock can be the most important factor in preventing WSD. Regarding biosecurity principals purchased feed must be free of shrimp head powder. Construction the new shrimp farms should be as far as it could be away from contaminated areas.
    Keywords: Aquaculture ; Ecology ; Health ; Iran ; Khouzestan Province ; Boushehr province ; Sistan and Balochestan province ; Hormozgan Province ; Epidemiological study ; WSD ; Fenneropenaeus indicus ; Penaeus vannamei ; Shrimp ; White Spot Disease ; Temperature ; Salinity ; PCR ; Polymerase Chain Reaction ; Ammonia ; Nitrogen ; Dissolved oxygen ; pH ; Transparency
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 468
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  • 3
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    Monitoring infectious agents in L.vannamei farms in choebdeh-Abadan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25262 | 18721 | 2018-09-07 07:28:07 | 25262 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The introduction of L. vannamei to Iran from Hawaii was initiated when high mortality occurred in shrimp farms of Abadan in 2002 than in Bushehr during summer of 2004. Inspection of L. vannamei for infectious agents (Bacteria , virus , fungi and parasite ) , determine total plate count and total vibrio in Bahmanshir Rivers, shrimps and farms water , examination physicochemical factors of farms water , Identification of carriers of wssv in wild shrimp and crabs , the main objectives of this study , which was held in 2007 . 540 shrimp samples (pl12 pl15) and subadults were collected from different shrimp farms in khouzestan province and then check for identity bacteria, fungi and parasite. Also 280 samples of L. vannamei , wild shrimps ( Metapenaeus af inis , Exopalaemon styliferus ) and crabs (Grapsus sp .and Sesarma sp. ) were Collected from Bahmanshir river for virology studies by PCR procedure (Iq2000 kit WSSV , IHHNV , TSV ) . 120 samples of L. vannamei for histopathology had been collected randomly and preserved in Davidson’s fixation and then transferred to 75% ethyl alcohol for storage. (Hepatopancreas gills). Physicochemical parameters of water in culture ponds comprising of pH DO, BOD, NO_3, NO_2, salinity. Total. Hardness and NH3 were measured all over culture period for 340 times. Finally it has been detected 10 genus and species of bacteria consisting of V . alginolyticus, V. parahaemolyticus, V. proteolyticus , plesiomonas shigelloides ; 6 genus and species of fungi specially Aspergilus niger , Asp.fumigatus and Asp.flavus and two genus of parasites vorticella sp. And Zoothamnium sp. were isolated. Crab (Sesarma sp.) were tested by using Iq2000 diagnostic kit for WSSV that positive for WSSV. There was a positive result (three viruses TSV, IHHNV and WSSV) for postlarvae and subadults of L.vannamei by using Iq2000 kit and IFRO kit (Internal kit). Histopathological studies have shown inclusion bodies of TSV, WSSV, IHHNV, MBV and HPV in various tissues. Results demonstrated the mean of vibrio count (0. 01×103 - 1.96 ×103) and tolal plate count (0 .21 ×103 - 14.25 ×10 3). physicochemical parameters of water were measured as follows : salinity (12.3 22.5 ppt ).BOD5 ( 1.98 10.21 ppm ) , DO (3.17 11.25 ppm ) , NH3(0.02 3.45 ppm ) and temperature (20 31. 5 c) . Keywords: L.vannamei, bacteria, virus, fungi, parasite Histo pathology - physicochemical parameters of water- Abadan khouzestan province.
    Keywords: Aquaculture ; Iran ; Khouzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; L.Vannamei ; Bacteria ; Virus ; Fungi ; Parasite ; Histopathology ; Physicochemical parameters of water ; Mortality ; Shrimp ; Metapenaeus affinis ; Exopalaemon styliferus ; Grapsus sp. ; Sesarma sp. ; Aspergilus niger ; Asp. Fumigatus ; Asp. Flavus ; Temperature
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 78
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  • 4
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    Fish gene bank of the Persian Gulf and Oman Sea (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25779 | 18721 | 2018-10-13 08:34:12 | 25779 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti، Netuma thalassina, Netuma bilineata Carcharhinus leucas، Choerodon robustus، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively. The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank. This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Keywords: Biology ; Iran ; Persian Gulf ; Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 66
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  • 5
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    Fish gene bank of the Persian Gulf and Oman Sea (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
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