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  • Fish  (3)
  • 16S rRNA  (2)
  • 1
    Publication Date: 2021-05-19
    Description: Carp culture is the most important fisheries subsector. In Iran, warm water fish farming is based on Chinese carps, namely common carp (Cyprinus carpio), silver carp (Hypophtalmichthy molitrix), grass carp (Ctenophrynogodon idella) and bighead (Aristichthys nobilis) carp. These carps are easy to breed in hatcheries in large numbers at little cost, and are distributed to farmers to grow out in ponds or open waters. The characteristics of the carp farming industry in the four main fish farming provinces, Gilan, Mazandaran, Golestan and Khouzestan are different. In 1970, shilat (the fisheries organization of Iran) established a carp culture research station in Gilan province, while the first commercial facility for carp culture was established in 1969, supported by Romanian experts. The number of 5 hatchery in two province Khouzestan and Fars have been established. Production of carp fingerlings increased to 114 million pieces by 2003, of which only 15% is produced by shilat. Some 20 private hatcheries produced more than 85% of the carp seed. Stock deterioration in hatchery populations due to poor broodstock management and in breeding depression has bee observed in recent years in Iran. Retarded growth, reduction in reproductive performance, morphological deformities, increased incidence of diseases and mortalities of hatchery- produced seeds have been reported. Brood stock management as a substantial component of fish farming highly depended on hatchery managers, technicians and fish farmers who are maintaining, selecting, reproducing the locally available brood fish. This project was done in 2002-2003, At first statistical number of hatcheries and warm water farms were collected. Questionaries was filled to know what factors is measured in reproduction season by managers. It was observed that only 10 percent of personel were B.Sc and technician expert. At first broodstock supply was in mazandaran probince and also hatchery managers procurement and collected broodstock from cultured farms and other hatcheries. To decreas the stress MS222 was used by 50% of managers. Brood fish by bodyshape, colour and length were chosen or ommited. In fifty percent hatcheries sex ratio 2 : 1 was done. Also in 80% warm water fishes farms silver carp deficiency and deformities was observed. Ne in hatcheries of south and west south of Iran between 10-200 was observed. Recommendations: for chossing broodstock it should be borne have fast growth, goodbody conformation, disease resistance high fecundity. Also brood stock sufficient numbers contributing to an introduction. We also hope to be able to promote the more general message of good gentic management of aquaculture brood fish hatcheries of Iran
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Warm water ; Fish ; Common carp ; Grass carp ; Silver carp ; Bighead ; Hatchery ; Brood stock ; Management ; Ne ; Evaluation ; Gentic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 62pp.
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25779 | 18721 | 2018-10-13 08:34:12 | 25779 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti، Netuma thalassina, Netuma bilineata Carcharhinus leucas، Choerodon robustus، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page. The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively. The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank. This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Keywords: Biology ; Iran ; Persian Gulf ; Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 66
    Location Call Number Expected Availability
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  • 3
    Publication Date: 2021-05-19
    Description: Genetic studies and gene banks preparation can identify guidelines for improving diversity and population structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In this study, sampling was performed from the important studied species habitats areas for the species such as: Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ، Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus lepturus. Total DNA extraction was performed using phenol - chloroform method which is the most common method for DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this study. After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to calculate genetic distance according to the Kimura 2-parameter model of sequence evolution. Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2, indicating good quality DNA samples. Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes 16SrRNA, making identification and registration of specified computer storage and regulate the structure and management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in compare to the samples recorded in the World gene Bank can considered as a major achievements of this research.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Gene Bank ; mtDNA ; Fish ; Marine ; 16S rRNA ; Dasyatis bennetti ; Netuma thalassina ; Netuma bilineata ; Carcharhinus leucas ; Choerodon robustus ; Pseudorhombus pentophthalmus ; Pseudorhombus arsius ; Thunnus tonggol ; Euthynnus affinis ; Trichiurus lepturus ; Species
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
    Location Call Number Expected Availability
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