Publication Date:
2021-05-19
Description:
Genetic studies and gene banks preparation can identify guidelines for improving diversity and population
structure and estimation, poaching and also the amount of cross breeding and provide genetic classification. In
this study, sampling was performed from the important studied species habitats areas for the species such as:
Dasyatis bennetti ، Netuma thalassina, Netuma bilineata Carcharhinus leucas ،Choerodon robustus ،
Pseudorhombus pentophthalmus ،Pseudorhombus arsius Thunnus tonggol ،Euthynnus affinis and Trichiurus
lepturus.
Total DNA extraction was performed using phenol - chloroform method which is the most common method for
DNA extraction in order to achieve high quality of DNA was performed in the preparation of gene bank in this
study.
After relevant studies on this gene primers were designed and in use. After editing the sequences, nucleotide
BLAST (Basic Local Alignment Search Tool) was performed using NCBI blast main page.The sequences
obtained from each sample were aligned and corrected from any ambiguities and assembled using Bio edit
program .Trees were generated using maximum parsimony (MP), a character-based algorithm and neighbor
joining (NJ) a distance-based algorithm for phenetic analysis. The distance matrix option of MEGA4 was used to
calculate genetic distance according to the Kimura 2-parameter model of sequence evolution.
Based on the results obtained, the optical density of 260 to 280 nm in the samples was recorded between 1/8 - 2,
indicating good quality DNA samples.
Optimized PCR reaction to 16SrRNA gene amplification using the gradient between 48 - 60° C showed that the
most suitable criteria for binding primers, 54 to 58 Celsius degrees respectively.The project objectives including
the identification of the genetic structure of the species, and draw the phylogenetic trees using two genes
16SrRNA, making identification and registration of specified computer storage and regulate the structure and
management of mentioned species by focus on genetic resources 10 species of majour commercial and noncommercial
fishes in the Persia Gulf and Oman Sea through the creation of an integrated network of aquatic
genetic resources in the region to try to identify genetic resources and aquatic gene bank.This type of analysis
could be considered as an important tool to be used in broodstock selection in breeding programs. Also identify
the species of fish named as Pseudorhombus pentophthalmus with a new haplotype and genetic differences 7% in
compare to the samples recorded in the World gene Bank can considered as a major achievements of this
research.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Gene Bank
;
mtDNA
;
Fish
;
Marine
;
16S rRNA
;
Dasyatis bennetti
;
Netuma thalassina
;
Netuma bilineata
;
Carcharhinus leucas
;
Choerodon robustus
;
Pseudorhombus pentophthalmus
;
Pseudorhombus arsius
;
Thunnus tonggol
;
Euthynnus affinis
;
Trichiurus lepturus
;
Species
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
66pp.
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