Publication Date:
1997-11-05
Description:
A biosensor has been developed based on induced wavelength shifts in the Fabry-Perot fringes in the visible-light reflection spectrum of appropriately derivatized thin films of porous silicon semiconductors. Binding of molecules induced changes in the refractive index of the porous silicon. The validity and sensitivity of the system are demonstrated for small organic molecules (biotin and digoxigenin), 16-nucleotide DNA oligomers, and proteins (streptavidin and antibodies) at pico- and femtomolar analyte concentrations. The sensor is also highly effective for detecting single and multilayered molecular assemblies.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lin, V S -- Motesharei, K -- Dancil, K P -- Sailor, M J -- Ghadiri, M R -- 2SO6 GM47165-06/GM/NIGMS NIH HHS/ -- GM18309/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1997 Oct 31;278(5339):840-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9346478" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Biosensing Techniques
;
Biotinylation
;
DNA, Complementary/analysis
;
Digoxigenin
;
*Interferometry
;
Mice
;
Sensitivity and Specificity
;
*Silicon
;
Streptavidin
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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